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38 protocols using sof lex

1

Finishing and Polishing Techniques for Dental Restorations

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Except for the control group, three different finishing and polishing systems were used.
• No polishing procedure was applied to the control group.
• In the second group, aluminium oxide-bonded discs (AOBD) (Enhance ® , Dentsply/DeTrey) and pastes were used for polishing.
• In the third group, the specimens were polished with a series of polishing discs (PD) (Sof-Lex™, 3M/ESPE, St. Paul, MN, USA) with a slow-speed handpiece (K10, Kavo, Leutkirch, Germany), applying each disk (coarse, medium, fine, and superfine) for 15 s.
• In the fourth group, a liquid polishing material (LPM) (BisCover™, Bisco, Schaumburg, IL, USA) was used according to the manufacturer's recommendations. All prepared specimens were stored in distilled water at 37°C for 24 h for rehydration and completion of the polymerization.
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2

Enamel Slab Preparation and Saliva Coating

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Bovine incisors were obtained, disinfected with 5% NaOCl 5%, and stored in 0.9% NaCl until use, not longer than 30 days. Enamel slabs (4 mm × 7 mm × 1 mm) were prepared using a diamond saw machine (LECO VC50 Diamond Saw, St. Joseph, MI, USA) and polished with sequential polishing disks (Soflex, 3M-ESPE, St. Paul, MN, USA). Initial surface hardness (SH)i was assessed by three linear indentations of a Knoop microindenter with a microhardness tester (402 MVD, Wolpert Wilson Instruments, Norwood, USA), at 50 g for 5 s. To control tissue variability, only slabs with microhardness of 364.19 ± 36.4 kg/mm2 (n = 18) were included. Samples were sterilized in an autoclave at 121°C for 15 min. Enamel slabs were covered for 30 min with ultra-filtered saliva obtained with 0.22 µm filters, from a healthy volunteer fasting for 12 h. To preserve the protein content and ensure the formation of the acquired pellicle on enamel to allow bacterial adhesion, saliva was mix with absorption buffer 0.1M containing phenylmethylsulfonyl fluoride 1:100 (v/v) and a protease inhibitor cocktail.[16 (link)] Slabs were suspended in the wells of a 24-well culture plate, with a metal holder made with orthodontic wire.
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3

Comparative Evaluation of Dental Restorative Materials

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A2 shade of Equia Forte (Fuji, GC) and Cention N (Ivoclar) were used in this study. Following the manufacturer's instruction, fifty samples were prepared for each of these materials and placed inside a metal ring. The metal ring was placed between two glass slabs for the removal of excess material [6 ]. All samples measured 10 mm in diameter and 4 mm in thickness. After the initial set, all samples were finished and polished. Polishing was done by using coarse, medium, fine, and superfine Soflex discs in sequence (Soflex system 3M, US). All Equia Forte samples received a layer of Equia Forte Coat and light cured using Coltolux (Coltene/Whaledent, USA) at 1400mW per-cm2 for 40 s at a distance of 2mm perpendicular to the sample surface using a light shield attached to the tip of handpiece. All samples were incubated at 37 degrees centigrade for 24 h. Each sample was placed on a black ground. The base-line L, a, and b values were recorded using a digital spectrophotometer (Vita EasyShade4.0, VITA, USA). As per the manufacturer's instructions, the spectrophotometer was manually calibrated before each set of measurements.
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4

Biodentine Restoration with Open Sandwich Technique

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The cavities were filled with Biodentine according to the manufacturer's instructions (Table 1). For the experimental group D0 which was divided into 8 subgroups (n = 5, 10 cavities), Biodentine was directly applied on the cavity walls. After 15 minutes, Biodentine was cut to leave a height of material of 2 mm to achieve a restoration of type “open sandwich” with the composite resin. The specimens were then restored using the adhesive system/composite resin according to the manufacturer's instructions (Table 1).
For experimental group D7 which was also divided into 8 subgroups (n = 5, 10 cavities), Biodentine was directly applied on the cavity walls, but after 15 minutes, samples were stored in an artificial Ringer solution. After 7 days, Biodentine cement was then cut to leave a height of material of 2 mm to achieve a restoration of type “open sandwich” with the composite resin. The specimens were then restored using the adhesive system/composite resin according to the manufacturer's instructions (Table 1).
Polymerization was achieved using a halogen light-curing unit (Elipar Highlight, 3M ESPE, Monrovia, CA, USA). The irradiance tested using a curing radiometer was 750 mW/cm2 and was consistent during the entire procedure. The restorations were polished with disks (Soflex, 3M ESPE) and the root apices were sealed using Ceram X Mono composite resin.
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5

Thermocycling Reattached Teeth

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Reattached teeth were finished and polished with the flexible polishing disk (Sof-Lex, 3 M ESPE, USA) and were then stored in artificial saliva for 48 h in an incubator adjusted at 37 °C, then specimens were subjected to thermo-cycling between 5 °C and 55 °C (±5 °C) for 500 cycles with 30 s dwell time [7 ].
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6

Finishing and Polishing of Silorane-based Composite

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The main factors evaluated in this in vitro study were finishing/polishing systems at four levels—control (light-cured in contact with polyester strip), aluminum oxide discs (Sof-Lex, 3M ESPE), diamond-impregnated silicone tips (Astropol, Ivoclar Vivadent), and aluminum oxide-impregnated silicone tips (Enhance, Dentsply)—and the time to perform finishing/polishing at two levels (immediately and after 7 days). The specimens were made of silorane-based composite (Filtek P90, 3M ESPE) following a randomized complete block design. The dependent variables were mean surface roughness (Ra, μm) (n = 20), Vickers microhardness (n = 10), and microleakage at the enamel and dentin margins, evaluated by dye penetration scores (n = 10). The surfaces of the specimens were analyzed using scanning electron microscopy (SEM). Table 1 shows the composition, batch, and manufacturer of the studied materials.
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7

Finishing and Polishing of Dental Restorations

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After removal from the acrylic molds, all the samples were finished using a Sof-Lex finishing and polishing system (Batch No. NC11342, 3M ESPE, MN, USA). This system is composed of two disposable wheels made of a thermoplastic elastomer impregnated with aluminum oxide particles. The beige spiral wheel is recommended for finishing, smoothing and removing the scratches produced during the restoration stages, while the white wheel is recommended for final polishing. During the finishing and polishing stage, each spiral wheel was used only once for each sample, and the procedure was performed for 1 min per sample (30 s for each wheel). The wheels were activated by a contra-angle handpiece at a speed of 20,000 revolutions per minute, according to the recommendations offered by the manufacturer.
After that, forty samples from each group were subjected to submersion in hydrochloric acid. Ten samples from groups A, B and C were maintained as they resulted after finishing and polishing procedure (subgroup 1) and twenty samples were exposed to a toothbrushing simulation process (subgroups 4i and 4ii).
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8

Composite Curing and Polishing Protocols

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In Group I, Filtek P60 composite was inserted into the mold in two increments of thickness 1.5 mm and light cured (Blue phase C8 LED, Ivoclar Vivadent, Schaan Liechtenstein, USA). In Group II, Filtek Bulk-Fill composite was placed into the mold as a single increment (thickness 3 mm) and light cured. The dimensions of the discs were 10 mm in diameter and 3 mm in thickness. A calibrated radiometer (Blue phase, Ivoclar Vivadent, Schaan Liechtenstein, USA) was employed to verify the efficacy of the light-curing unit. After complete curing, each specimen was polished with a multistep polishing system, Sof-Lex (3M ESPE, St. Paul, MN, USA) in a sequential manner. The polishing procedure was standardized by applying light pressure for 15 s.
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9

In-Situ Composite Resin Cylinder Formation

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Transparent plastic tube was then fixed manually on the prepared surface of each tooth. A microhybrid composite resin (SwisTec, COLTENE, Switzerland) was applied in five 1 mm layers, and each layer was photopolymerized for 40 s, reaching 5 mm in total height, during which the light was moved around the tube to assure curing of the entire composite resin cylinder. The plastic tube was then removed, and excess composite resin was removed using a polishing disc (Sof-Lex™; 3M Espe, St. Paul, MN, USA). The samples were then stored in distilled water at 37°C for 48 h.
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10

Composite Specimen Preparation and Polishing

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A total of 40 cylindrical composite specimens were made (6 mm diameter, height of 2 mm for conventional and 4 mm for bulk-fill composites), 8 samples for each composite (n = 8). Specimens were made in metal molds, open on both sides, on the bottom side flattened with glass plates, and on the top side covered with Mylar foils to obtain the smooth surface needed for the proper measurement of the MH [23 (link)]. Composite specimens were irradiated only from the top side (that was marked), according to the described curing protocols, and were stored in a dry and dark place for 24 h to complete the post-cure reaction [24 (link)]. The samples were subsequently polished on the top and bottom with a four-step coarse to superfine grain disc system (20 s per step) (Sof-Lex, 3M ESPE, St. Paul, MN, USA) at a speed of 15,000 rpm. Final polishing was performed with the Sof-Lex diamond polishing system, which consists of pre-polishing and diamond-impregnated polishing spirals that achieve a highly polished surface. Surface residues were removed by washing and drying the samples.
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