Human il 18 elisa kit

S100B, NSE, IL-6, IL-10, IL-18, and MMP-9 were measured according to the kit's instructions. The absorbance of each well was measured at 450 nm using a microplate reader. The human IL-18 ELISA kit (D7620; R&D Systems, Minneapolis, MN, USA), human IL-10 ELISA kit (D1000B; R&D Systems, Minneapolis, MN, USA), human MMP9 ELISA kit (D6000; R&D Systems, Minneapolis, MN, USA), human S100B ELISA kit (DY1820-05; R&D Systems, Minneapolis, MN, USA), and human NSE ELISA kit (DENL20; R&D Systems, Minneapolis, MN, USA) were used.

Serum IL-18 levels were measured by Human IL-18 ELISA kit (R&D systems, USA) according to manufacturer’s instructions. The limits of detection of the assay were at 12.5 pg/mL. Active serum IL-1β levels were measured using Quantikine HS Human IL-1β immunoassay (R&D systems, USA) according to manufacturer’s instructions. The sensitivity ranges from 0.023 pg/mL to 0.14 pg/mL. The limits of detection in this assay was at 0.125 pg/mL.

All commercial antibodies and chemicals were purchased from the following sources: anti‐HA (51064‐2‐AP), anti‐NF‐κB p65 (10745‐1‐AP), anti‐Lamin B1 (12987‐1‐AP) and anti‐β‐actin (Proteintech, 60008‐1‐lg) antibodies and human TNFα (HZ‐1014) were manufactured by Proteintech; anti‐Pin1 (ab192036), anti‐NF‐κB p65 (ab19870) and anti‐IL‐18 (ab71495) antibodies were purchased from Abcam; anti‐flag antibody (F1804) was purchased from Sigma; anti‐NF‐κB p65 (Thr254) (11010) was purchased from Signalway Antibody Co.; anti‐NF‐κB p65 (Ser276) (AF3387) antibody was obtained from Affinity Biosciences; and human IL‐18 ELISA kit and recombinant human IL‐18 (rhIL‐18) were purchased from R&D Systems.