Rabbit lrrk2 mjff2

Manufactured by Abcam

Sourced in United Kingdom

Rabbit LRRK2 MJFF2 is a recombinant protein that represents a specific fragment of the Leucine-rich repeat kinase 2 (LRRK2) enzyme. It is produced in a rabbit expression system.

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Lab products found in correlation

Rabbit lrrk2 phospho s935, by Abcam (2 mentions) Rabbit lrrk2 phospho t2483, by Abcam (2 mentions) Rabbit lrrk2 phospho t1491, by Abcam (2 mentions) Mouse c myc 9e10, by Roche (2 mentions) Rabbit phospho pak4 5 6, by Merck Group (2 mentions) Mouse limk1, by Abcam (2 mentions) Rabbit lrrk2 udd3, by Abcam (2 mentions) Mouse c myc, by Roche (2 mentions)

Close spelling variants of this product

MJFF2 LRRK2

2 protocols using rabbit lrrk2 mjff2

Immunoblotting Analysis of LRRK2 Signaling

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For immunoblotting analysis the following antibodies were used: rabbit LRRK2 MJFF2 (Epitomics Cat# 3514-1, RRID:AB_10643781, 1:100), rabbit LRRK2 phospho-S935 (Epitomics Cat# 5099-1, RRID:AB_ 11132319, 1:100), rabbit LRRK2 phospho-T2483 (Abcam Cat#156577, 1:2000), rabbit LRRK2 phospho-T1491 (Abcam Cat#140106, 1:2000), mouse Flag M2 (Sigma, Cat# F1804, 1:10000), mouse c-Myc 9E10 (Roche, Cat# 11667149001, RRID:AB_ 390912, 1:5000), rabbit PAK6 (Prestige® Sigma, Cat# HPA031124, RRID:AB_10601044, 1:2000), mouse β-tubulin (Sigma, Cat# T8328, RRID:AB_1844090, 1:5000), rabbit phospho-PAK4-5-6 (Sigma, Cat# SAB4504052, 1:2000), rabbit phospho-LIMK1 (Cell Signaling, Cat#3841, 1:1000), mouse LIMK1 (Abcam, Cat#117623, 1:1000).
For immunoprecipitation, the following antibodies were used: rabbit LRRK2 UDD3 (Epitomics, Cat# 5097-1, 1 μg/mg total proteins), mouse c-Myc (9E10, Roche, Cat# 11667149001, RRID:AB_390912, 0.8 μg/mg total proteins), mouse Flag M2 (Sigma, Cat# F1804, 1 μg/mg total proteins).

Civiero L., Cirnaru M.D., Beilina A., Rodella U., Russo I., Belluzzi E., Lobbestael E., Reyniers L., Hondhamuni G., Lewis P.A., Van den Haute C., Baekelandt V., Bandopadhyay R., Bubacco L., Piccoli G., Cookson M.R., Taymans J, & Greggio E. (2015). Leucine‐rich repeat kinase 2 interacts with p21‐activated kinase 6 to control neurite complexity in mammalian brain. Journal of Neurochemistry, 135(6), 1242-1256.

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Immunoblotting Analysis of LRRK2 Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunoblotting analysis the following antibodies were used: rabbit LRRK2 MJFF2 (Cat# 3514‐1, RRID:AB_10643781, 1 : 100; Epitomics), rabbit LRRK2 phospho‐S935 (Cat# 5099‐1, RRID:AB_ 11132319, 1 : 100; Epitomics, Cambridge, UK), rabbit LRRK2 phospho‐T2483 (Cat#156577, 1 : 2000; Abcam, Cambridge, UK), rabbit LRRK2 phospho‐T1491 (Cat#140106, 1 : 2000; Abcam,Cambridge, UK), mouse Flag M2 (Cat# F1804, 1 : 10000; Sigma), mouse c‐Myc 9E10 (Cat# 11667149001, RRID:AB_ 390912, 1 : 5000; Roche Molecular Biochemicals, Indianapolis, IN, USA), rabbit PAK6 (Cat# HPA031124, RRID:AB_10601044, 1 : 2000, Prestige^®; Sigma), mouse β‐tubulin (Cat# T8328, RRID:AB_1844090, 1 : 5000; Sigma), rabbit phospho‐PAK4‐5‐6 (Cat# SAB4504052, 1 : 2000; Sigma), rabbit phospho‐LIM domain kinase 1 (LIMK1) (Cat#3841, 1 : 1000; Cell Signaling Technology, Beverly, MA, USA), mouse LIMK1 (Cat#117623, 1 : 1000; Abcam).
For immunoprecipitation, the following antibodies were used: rabbit LRRK2 UDD3 (Cat# 5097‐1, 1 μg/mg total proteins; Epitomics), mouse c‐Myc (9E10, Cat# 11667149001, RRID:AB_390912, 0.8 μg/mg total proteins; Roche), mouse Flag M2 (Cat# F1804, 1 μg/mg total proteins; Sigma).

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