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Ab194678

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Ab194678 is a laboratory equipment product. It serves a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Anti h3k27me3, by Merck Group (2 mentions) Pcmv ha jmjd3 plasmid, by Addgene (2 mentions) Anti histone h3, by Cell Signaling Technology (2 mentions) Ab8895, by Abcam (2 mentions) Ab1012, by Abcam (2 mentions) Ab8898, by Abcam (2 mentions) Ab1220, by Abcam (2 mentions) Anti ha, by Santa Cruz Biotechnology (2 mentions)

2 protocols using ab194678

1

Quantifying Epigenetic Modifications in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
pCMV-HA-JMJD3 plasmid was obtained from Addgene (#24167). Lentiviral shRNAs (Extended Data Table 5) were provided by the Vector Core at the University of Michigan or kindly provided by Dr. Arul Chinnaiyan (University of Michigan). Antibodies including monoclonal anti-EZH2 (1:2000, BD Biosciences, 612667), anti-H3K27me3 (1:1000, Millipore, 07-449), H3K9me2 (1:2000, ab1220, Abcam), H3K9me3 (1:500, ab8898, Abcam), H3K4me1 (ab8895, Abcam), H3K4me2 (ab194678, Abcam), H3K4me3 (ab1012, Abcam), anti-Histone H3 (1:2000, Cell Signaling, 9715), anti-HA (1:200, Santa Cruz Biotechnology, sc-805), anti-DNMT1 (1:250, Abcam, ab13537) were used for Western blotting. Anti-human CXCR3 (1C6) blocking antibody was prepared from mouse hybridoma (ATCC, #HB-12330) by Hybridoma Core at the University of Michigan.
Peng D., Kryczek I., Nagarsheth N., Zhao L., Wei S., Wang W., Sun Y., Zhao E., Vatan L., Szeliga W., Kotarski J., Tarkowski R., Dou Y., Cho K., Hensley-Alford S., Munkarah A., Liu R, & Zou W. (2015). Epigenetic silencing of Th1 type chemokines shapes tumor immunity and immunotherapy. Nature, 527(7577), 249-253.
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2

Quantifying Epigenetic Modifications in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
pCMV-HA-JMJD3 plasmid was obtained from Addgene (#24167). Lentiviral shRNAs (Extended Data Table 5) were provided by the Vector Core at the University of Michigan or kindly provided by Dr. Arul Chinnaiyan (University of Michigan). Antibodies including monoclonal anti-EZH2 (1:2000, BD Biosciences, 612667), anti-H3K27me3 (1:1000, Millipore, 07-449), H3K9me2 (1:2000, ab1220, Abcam), H3K9me3 (1:500, ab8898, Abcam), H3K4me1 (ab8895, Abcam), H3K4me2 (ab194678, Abcam), H3K4me3 (ab1012, Abcam), anti-Histone H3 (1:2000, Cell Signaling, 9715), anti-HA (1:200, Santa Cruz Biotechnology, sc-805), anti-DNMT1 (1:250, Abcam, ab13537) were used for Western blotting. Anti-human CXCR3 (1C6) blocking antibody was prepared from mouse hybridoma (ATCC, #HB-12330) by Hybridoma Core at the University of Michigan.
Peng D., Kryczek I., Nagarsheth N., Zhao L., Wei S., Wang W., Sun Y., Zhao E., Vatan L., Szeliga W., Kotarski J., Tarkowski R., Dou Y., Cho K., Hensley-Alford S., Munkarah A., Liu R, & Zou W. (2015). Epigenetic silencing of Th1 type chemokines shapes tumor immunity and immunotherapy. Nature, 527(7577), 249-253.
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