Breast cell lines BT20, MCF10A, MCF7, MDA-MB231, HS578T, and SKBR3 were obtained from the American Type Culture Collection and were grown as previously described (73 (link)). HMEC line 184A1 was obtained from M. Stampfer (Lawrence Berkeley National Laboratory) and maintained in DFCI-1 medium as previously described (74 (link)). Cells were plated in 15-cm dishes or 96-well plates, grown for 24 hours, starved in serum-free medium for 18 hours, and starved again for an additional hour before treatment. Primary NHDF cells were obtained from Lonza and cultured to confluence in Fibroblast Growth Media-2 (Lonza).
Nhdf cells
Manufactured by Lonza
NHDF cells are primary human dermal fibroblasts derived from normal, healthy human skin. These cells are widely used in cell culture research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 7, by American Type Culture Collection (2 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Skbr3, by American Type Culture Collection (1 mentions)
Hs578t, by American Type Culture Collection (1 mentions)
Bt 20, by American Type Culture Collection (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
Dmem f2, by Thermo Fisher Scientific (1 mentions)
Penicillin g, by Corning (1 mentions)
Synergy h1 plate reader, by Agilent Technologies (1 mentions)
Celltiter glo assay, by Promega (1 mentions)
Amphotericin b, by Corning (1 mentions)
Pfsk1 cells, by American Type Culture Collection (1 mentions)
Fgm 2 medium, by PromoCell (1 mentions)
5 protocols using nhdf cells
1
Diverse Breast Cell Line Culturing
2
Cell Culture Protocols for HCT 116, MCF-7, and NHDF
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HCT 116 and MCF-7 cells were purchased from the American Type Culture Collection. NHDF cells were purchased from Lonza. All cells were cultured under standard conditions at 37 °C in a humidified atmosphere at 5% CO2 in DMEM/F12 medium (PAA) supplemented with 10% FBS (EURx).
3
Generation of Induced Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Direct reprogramming experiments were performed by transducing Normal Human Dermal Fibroblasts (NHDF cells, Lonza) with RTTA, tetO-Ascl1, tetO-Brn2 and tetO-Myt1l (BAM) in a 1.2:1:1.5:2.5 ratio and supplemented with polybrene (c.f. 8 μg/ml) 24 h before siRNA transfection to deplete FACT. Induction of BAM by DOX supplementation (2 μg/ml) was started the next day in neuronal medium ((DMEM/F2 (Invitrogen), apotransferrin (100mg/ml), insulin (5mg/ml), sodium selenite (30 nM), progesterone (20 nM), putrescine (100 nM), penicillin/streptomycin) supplemented with neurotrophic factors) as described before (Vierbuchen et al., 2010 (link)).
4
Evaluating NHDF Cytocompatibility Using Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In vitro cytocompatibility was assessed using a modified protocol[18 (link)] (Figure 1A ). Normal human dermal fibroblast (NHDF) cells (Lonza, Walkersville, MD) were seeded at 1.0 × 104 cells/cm2 in 12-well tissue culture plates in 1.5 mL of Dulbecco’s Modified Eagle’s Medium solution supplemented with 10% fetal bovine serum and 1 × antibiotic-antimycotic solution (100 units/mL Penicillin G, 100 μg/mL streptomycin sulfate, 0.25 μg/mL amphotericin B, Corning Inc., Manassas, VA). Twenty-four hours after cell seeding, hydrated paste samples (0.5 mL) and control sponge samples (8 mm diameter) were added to cell culture inserts (8 μm pore membrane, Corning Inc., Manassas, VA) (n = 5/group at each time point), and placed into wells containing NHDF cells and media. To asses NHDF viability, at 24 and 72 h post-exposure a Cell Titer-Glo® assay (Promega, Madison, WI) was used to measure cell viability using a BioTek Synergy H1 plate reader (Winooski, VT). Tissue culture plastic controls from each time point were used to calibrate luminescence values to a cell viability relative to the tissue culture plastic.
5
Cell Culture Protocols for Multiple Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293 (46 (link)), H1299 (47 (link)), and HeLa SUMO1/2 and HeLa Par cells (39 (link)) were grown as monolayer cultures in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 5% penicillin/streptomycin. PFSK-1 cells (ATCC; CRL-2060) were grown in RPMI medium supplemented with 10% FBS,5% penicillin/streptomycin, and l -glutamine. nHDF cells (Lonza) were cultured in FGM-2 medium (PromoCell) supplemented with the corresponding BulletKit.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!