Anti chga

Manufactured by Abcam

Anti-ChgA is a laboratory reagent used for the detection and quantification of chromogranin A (ChgA) in various biological samples. ChgA is a widely used biomarker for neuroendocrine tumors and other conditions. This antibody-based product can be utilized in techniques such as ELISA, Western blotting, and immunohistochemistry to aid in the study of ChgA expression and its role in research and clinical applications.

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Lab products found in correlation

Anti hes1, by Abcam (1 mentions) Anti ph3, by Cell Signaling Technology (1 mentions) Anti c caspase3, by Cell Signaling Technology (1 mentions) Anti parp 46d11, by Cell Signaling Technology (1 mentions) Anti ki67 b56, by BD (1 mentions) Anti 8 ohdg, by Abcam (1 mentions) Anti lc3a b d3u4c, by Cell Signaling Technology (1 mentions) Anti dclk1, by Abcam (1 mentions) Anti claudin 1, by Cell Signaling Technology (1 mentions) Anti pcna, by Santa Cruz Biotechnology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti brg1 epncir111a, by Abcam (1 mentions) Anti caspase 3 8g10, by Cell Signaling Technology (1 mentions) Anti zo 1, by Thermo Fisher Scientific (1 mentions) Anti trpa1, by Novus Biologicals (1 mentions) Fluoromount g, by Thermo Fisher Scientific (1 mentions) Anti st2, by Proteintech (1 mentions)

2 protocols using anti chga

Comprehensive Antibody Characterization Protocol

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The primary antibodies used in this study were as follows: anti-BRG1 (EPNCIR111A) Abcam Cat# ab110641 (1:10,000); anti-Caspase3 (8G10), Cell Signaling Technology, Cat# 9665 (1:1000); anti-C-Caspase 3, Cell Signaling Technology, Cat# 9661 (1:1000); anti-PARP (46D11), Cell Signaling Technology, Cat# 9532 (1:1000); anti-Ki67 (B56), BD Biosciences, Cat# 550609 (1:500); anti-EpCAM (G8.8), BD Biosciences, Cat# 552370 (1:500); anti-ZO1, Invitrogen, Cat# 40-2200 (1:500); anti-8-OHd G, Abcam, Cat# ab48508 (1:500); anti-LC3A/B (D3U4C), Cell Signaling Technology, Cat# 12741 (1:1000); anti-ATG16L1 (D6A5), Cell Signaling Technology, Cat# 8089 (1:1000); anti-PCNA, Santa Cruz Biotechnology, Cat# SC-7909 (1:1000); anti-P-H3, Cell Signaling Technology, Cat# 9701 (1:1000); anti-E-Cadherin, Cell Signaling Technology, Cat# 3195T (1:1000); anti-DCLK1, Abcam, Cat# ab31704 (1:500); anti-Claudin1, Cell Signaling Technology, Cat# 4933T (1:1000); anti-ChgA, Abcam, Cat# ab715 (1:1000); anti-Hes1, Abcam, Cat# ab71559 (1:500). Raw data of immunoblotting can be found in the source data file.

Liu M., Sun T., Li N., Peng J., Fu D., Li W., Li L, & Gao W.Q. (2019). BRG1 attenuates colonic inflammation and tumorigenesis through autophagy-dependent oxidative stress sequestration. Nature Communications, 10, 4614.

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Intestinal Goblet and Enteroendocrine Cell Analysis

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Mouse colon was fixed in 10% neutral formalin and stored in 70% ethanol. Intestinal samples were then paraffin-embedded and cut into 10 um longitudinal sections, and the PAS staining was performed by Histoserv Inc. (Germantown, MD). Goblet cells were counted and calculated from 10 crypts in each slide.
For immunofluorescence staining, slides were deparaffinized by xylene and antigen retrieval was conducted for 20 min in a 95°-water bath in 10 mM sodium citrate, pH 6.0 followed by a 15 min incubation at room temperature. Slides were washed, blocked in 5% bovine serum albumin, and stained with the primary antibodies, anti-ChgA (1:100; Abcam), anti-5-HT (1:100; Abcam), anti-ST2 (1:100, Proteintech) and anti-TRPA1 (1:100, Novus) and secondary antibodies conjugated to Alexa fluor 488, 633 or 594 (Thermo Fisher). Slides were mounted in Fluoromount-G (Thermo Fisher), and 3–15 images were taken per slide at 20X or 40X magnification along transections of the intestinal crypts for each biological replicate (Zeiss). For 5-HT and ChgA staining, numbers of positively stained puncta were scored blindly, normalized to total area of intestinal mucosa using ImageJ software (NIH), and then averaged across biological replicates.

Chen Z., Luo J., Li J., Kim G., Stewart A., Urban JF J.r., Huang Y., Chen S., Wu L.G., Chesler A., Trinchieri G., Li W, & Wu C. (2020). Interleukin-33 promotes serotonin release from enterochromaffin cells for intestinal homeostasis. Immunity, 54(1), 151-163.e6.

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