Agilent 7890b gc system

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 7890B GC system is a gas chromatography instrument designed for the separation, identification, and quantification of chemical compounds in complex samples. It features a robust and reliable design, providing precise and reproducible results.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Agilent 7890B (233 citations) 7890B GC system (121 citations) Agilent 7890B GC (88 citations) GC 7890B (81 citations) 7890B system (12 citations) Agilent 7890B system (7 citations) GC systems (3 citations) 7890B GC-Agilent 7010 Triple Quadrapole Mass Spectrometer system (2 citations) Agilent 7890B GC-MS system (2 citations) Agilent 7890B-5977B GC-MS system (2 citations)

67 protocols using agilent 7890b gc system

Quantitative GC-MS Analysis of Cannabinoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The BAL fluids were analyzed using an Agilent 7890B GC System with an Agilent Sampler 80 and interfaced Agilent 7000C triple-quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA). Chromatographic separation was achieved using a HP-5MS UI (30 mm × 0.25 µm x 0.25 mm) capillary column with a 1.2 ml/min flow of helium carrier gas. GC inlet was operated at 250°C in split mode with a 20:1 split ratio. The initial oven temperature was 150°C and was ramped at 40°C/min to 250°C with a 2 min hold, followed by a second ramp of 5°C/min until reaching a maximum temperature of 300°C. Analytes eluted at 7.693 min (CBN-D³), 8.05 min (SQA), and 9.89 min (SQE) with a total run time of 14.5 min. The mass spectrometer source and transfer line were maintained at 280°C and quadrupoles temperatures were 150°C. The MS was operated in positive electron ionization (EI) mode and the resulting ions analyzed by single-ion monitoring (SIM) scan mode. Electron multiplier voltage gain factor was 10 for all ions monitored (SQA: 113 m/z (quantitative), 183, 127, 422 m/z (confirmation); SQE: 137 (quantitative), 410, 411 m/z (confirmation); CBN-D³ 298 m/z (quantitative), 313, 312 m/z (confirmation). Data acquisition and analysis were conducted using Agilent MassHunter Workstation Software (Agilent Technologies, Santa Clara, CA).

Cowan E.A., Tran H., Watson C.H., Blount B.C, & Valentín-Blasini L. (2022). The Quantitation of Squalene and Squalane in Bronchoalveolar Lavage Fluid Using Gas Chromatography Mass Spectrometry. Frontiers in Chemistry, 10, 874373.

+ Open protocol

+ Expand

GC-MS Analysis of Amino Acids and Fatty Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

GC/MS analysis was performed on an Agilent 7890B GC system equipped with a DB-5MS capillary column (30 m, 0.25 mm i.d., 0.25 µm-phase thickness; Agilent J&W Scientific), connected to an Agilent 5977A Mass Spectrometer operating under ionization by electron impact (EI) at 70 eV. Helium flow was maintained at 1 mL/min. The source temperature was maintained at 230°C, the MS quad temperature at 150°C, the interface temperature at 280°C, and the inlet temperature at 250°C. For GC/MS analysis of amino acids, 1 µL was injected at 1:40 split ratio. The column was started at 80°C for 2 min, increased to 280°C at 7°C/min, and held for 20 min. For GC/MS analysis of fatty acid methyl esters (FAME) and sugar derivatives, 1 µL was injected splitless. The column was started at 80°C for 2 min, increased to 280°C at 10°C/min, and held for 12 min.

Long C.P, & Antoniewicz M.R. (2014). Quantifying Biomass Composition by Gas Chromatography/Mass Spectrometry. Analytical chemistry, 86(19), 9423-9427.

+ Open protocol

+ Expand

Quantifying Glucose and Fructose in Mouse Plasma and Liver

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood was collected from mice with ad libitum feeding in three dietary groups (regular chow diet, fructose-high diet, and fructose-restrict diet, Table S5). Liver tissues were frozen using a freeze clamp. Glucose and fructose levels in the plasma were measured by a modified GC-MS method (Antoniewicz et al., 2011 (link); Long and Antoniewicz, 2014 (link)). Briefly, internal standard of U-¹³C glucose or U-¹³C fructose were added for quantification. Protein in the plasma was precipitated with methanol. Samples were air-dried. Hydroxylamine/pyridine solution was added, and samples were then incubated for 60 min at 90°C, followed by adding propionic anhydride to react at 60°C for 30 min. Samples were then evaporated to dryness under airflow and re-dissolved in ethyl acetate for GC-MS analysis on the Agilent 7890B GC system. Glucose and fructose measurements used m/z 370 and m/z 401 respectively.
Liver insulin was extracted from snap frozen liver samples using Tissue Extraction Reagent I (FNN0071, ThermoFisher, USA). Insulin in both plasma and liver tissue was measured by UltraSensitive Mouse Insulin ELISA Kit (90080, Crystal Chem. Inc. USA), described previously (Kim et al., 2016 (link)).

Bu P., Chen K.Y., Xiang K., Johnson C., Crown S.B., Rakhilin N., Ai Y., Wang L., Xi R., Astapova I., Han Y., Li J., Barth B.B., Lu M., Gao Z., Mines R., Zhang L., Herman M., Hsu D., Zhang G.F, & Shen X. (2018). Aldolase B Mediated Fructose Metabolism Drives Metabolic Reprogramming of Colon Cancer Liver Metastasis. Cell metabolism, 27(6), 1249-1262.e4.

+ Open protocol

+ Expand

GC-MS Analysis of Derivatized Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

1.5 µL of each derivatized sample was injected in (1:5) split mode into an Agilent 7890 B GC system (Agilent Technologies, Santa Clara, CA) coupled to a Pegasus HT TOF-MS (LECO Corporation, St. Joseph, MI). Separation was achieved on an Rtx-5 w/Integra-Guard capillary column (30 m × 0.25 mm ID, 0.25 µm film thickness; Restek Corporation, Bellefonte, PA) with helium as the carrier gas, at a constant flow rate of 1.0 mL/minutes. The temperatures of injection, transfer interface and ion source were 150 °C, 270 °C and 320 °C, respectively. GC temperature programming was set to 0.2 minutes of isothermal heating at 70 °C, followed by 6 °C /minutes oven temperature, ramping to 300 °C, a 4.0 minute isothermal heating of 270 °C, 20 °C/minute to 320 °C and a 2.0 minute isothermal heating of 320 °C. Electron impact ionization (70 eV) at full scan mode (40–600 m/z) was used, with an acquisition rate of 20 spectra per second in the TOF/MS setting.
Peak picking and alignments were performed using ChromaTof 4.7.2 (LECO Corporation). Mass spectra were compared to literature spectra available in the NIST database as well as the Fiehn library of compounds. A β-hydroxybutyric acid pure chemical standard was purchased (catalog #166898, Sigma-Aldrich, St. Louis, MO) for validation.

Hinzman C.P., Baulch J.E., Mehta K.Y., Girgis M., Bansal S., Gill K., Li Y., Limoli C.L, & Cheema A.K. (2019). Plasma-derived extracellular vesicles yield predictive markers of cranial irradiation exposure in mice. Scientific Reports, 9, 9460.

+ Open protocol

+ Expand

GC-MS Analysis of Supernatant

Check if the same lab product or an alternative is used in the 5 most similar protocols

Eighty μL of the supernatant were transferred to an auto-sampler vial for gas chromatography–mass spectrometry (GC–MS) analysis. GC separation was conducted by Agilent 7890B GC System (Agilent Technologies, CA, United States). Mass spectrometry was performed with an EI source with selected ion monitoring using an Agilent 5977A mass spectrometer (Agilent Technologies, CA, United States). Ion source capillary temperature was 230°C.

Lyu X., Wang S., Zhong J., Cai L., Zheng Y., Zhou Y., Zhou Y., Chen Q, & Li Q. (2023). Gut microbiome interacts with pregnancy hormone metabolites in gestational diabetes mellitus. Frontiers in Microbiology, 14, 1175065.

+ Open protocol

+ Expand

Fecal SCFA Quantification by GC-FID

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fecal concentrations of SCFAs were measured by means of gas chromatography coupled with a flame-ionization detector (GC-FID) as described by Zhang et al. [27 (link)]. Briefly, to extract SCFAs, 0.1 g fecal sample was vortexed in 1 mL distilled water and centrifuged. The supernatant of the centrifuged sample (150 μL) was transferred to a screw cap vial with 150 μL of GC buffer solution, which contains (NH₄)₂SO₄, NaH₂PO₄, and 2-ethylbutric acid. GC-FID analysis was performed by Agilent 7890B GC system equipped with a 7697A headspace sampler and FID (Agilent Technologies, Wilmington, DE, USA). An HP-innowax capillary column (30 m x 0.32 mm i.d. x 0.50 μL film thickness; Agilent Technologies) was used with constant flow of nitrogen as the carrier gas. Data acquisition and operation processing were conducted using ChemStation software (Agilent Technologies). Each SCFA was identified by its corresponding retention time and by spiking with standards in the same conditions. The amount of each SCFA was evaluated according to the peak area with the standard curve for quantitative analysis.

Lee C., Kim S., Kim B., Holzapfel W.H, & Hyun C.K. (2023). Disturbance of lipid metabolism in germ-free mice transplanted with gut microbiota of DSS-induced colitis mice. PLOS ONE, 18(2), e0280850.

+ Open protocol

+ Expand

GC-MS Analysis of Amino Acids and Glucose Labeling

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

GC-MS analysis was performed on an Agilent 7890B GC system equipped with a DB-5MS capillary column (30 m, 0.25 mm i.d., 0.25 μm-phase thickness; Agilent J&W Scientific), connected to an Agilent 5977A Mass Spectrometer operating under ionization by electron impact (EI) at 70 eV. Helium flow was maintained at 1 mL/min. The source temperature was maintained at 230°C, the MS quad temperature at 150°C, the interface temperature at 280°C, and the inlet temperature at 250°C. GC-MS analysis of tert-butyldimethylsilyl (TBDMS) derivatized proteinogenic amino acids was performed as described in (Long and Antoniewicz, 2014b (link)). Labeling of glucose was determined using the aldonitrile propionate derivatization method described in (Antoniewicz et al., 2011 (link)).

Crown S.B., Long C.P, & Antoniewicz M.R. (2016). Optimal tracers for parallel labeling experiments and 13C metabolic flux analysis: A new precision and synergy scoring system. Metabolic engineering, 38, 10-18.

+ Open protocol

+ Expand

GC-MS Analysis of Unique C. cinereum Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

In HPTLC analysis, an unambiguous band unique to C. cinereum was subjected to GC–MS experiments to identify the chemical. The analysis was performed on a GC/MS instrument (triple quadrupole GC/MS (GC-QQQ), Agilent Technologies, Agilent 7890B GC system and Agilent 7000C GC/MS Triple Quad) equipped with an HP-5 ms (length 30 m × inner diameter (ID) 0.25 mm, film thickness 0.25 µm) porous-layer open-tubular (PLOT) column. The split ratio was 1:10. The temperature of the injection port was 280 °C. The flow rate of the helium carrier gas was maintained at a constant 1.0 mL/min. The column temperature was held at 40 °C for 2 min to concentrate the hydrocarbon at the head of the column; subsequently, the column temperature was increased to 150 °C, ramped at 25 °C/min to 300 °C, and held at 300 °C for 15 min. The MS analyses were performed in full scan mode in electrospray ionization (EI) mode with a scan range of m/z 33–600. The ion source was maintained at 70 °C, and an ionization energy of 230 eV was used for each measurement. Compounds were identified by query mass spectrum matching with the NIST2011 reference library (National Institute of Standards and Technology, Gaithersburg, MD, USA).

Thongkhao K., Pongkittiphan V., Phadungcharoen T., Tungphatthong C., Urumarudappa S.K., Pengsuparp T., Sutanthavibul N., Wiwatcharakornkul W., Kengtong S, & Sukrong S. (2020). Differentiation of Cyanthillium cinereum, a smoking cessation herb, from its adulterant Emilia sonchifolia using macroscopic and microscopic examination, HPTLC profiles and DNA barcodes. Scientific Reports, 10, 14753.

+ Open protocol

+ Expand

GC/MS Analysis of AZ-SFE Compound

Check if the same lab product or an alternative is used in the 5 most similar protocols

GC/MS analysis of AZ-SFE was carried out on Agilent 7890B GC system coupled with 5977A mass selective detector (Agilent Technologies Inc., Santa Clara, CA, USA) in electronic ionization mode (ionization energy: 70 eV). The GC column was Agilent HP-5ms (30 m × 0.25 mm, 0.25 μm). AZ-SFE was dissolved in chloroform for analysis. The heating temperature was as follows: Hold at 50 °C for 5 min, rise to 170 °C at the rate of 10 °C/min, and hold for 5 min; then rise to 230 °C at the rate of 3 °C/min and hold for 3 min; finally rise to 280 °C at the rate of 5 °C/min and hold for 5 min. Inlet temperature and transmission line temperature were both 250 °C. Helium was used as carrier gas at a flow rate of 1 mL/min. The injection volume was 1 μL with a split ratio of 10:1. Ion source temperature was 230 °C and quadrupole the temperature was at 150 °C. The scan scale was 30–600 amu.

Liu J., Yu L., Mo N., Lan H., Zhang Y., Liu X, & Wu Q. (2019). Supercritical Fluid Extract of Angelica sinensis and Zingiber officinale Roscoe Ameliorates TNBS-Induced Colitis in Rats. International Journal of Molecular Sciences, 20(15), 3816.

+ Open protocol

+ Expand

GC-MS Analysis of Serum Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

The prepared serum samples were analyzed by an Agilent 7890B GC system (Agilent Technologies, Santa Clara, CA, USA) and a Leco TOF Pegasus BT mass spectrometry (LECO, St. Joseph, MI, USA). A DB-5MS capillary column (30 m length, 0.25 mm i.d, 0.25 µm film thickness; J &W Scientific, Folsom, CA, USA) was used for a helium gas flow of 1.5 mL/min. Then, 1 µL of the sample was injected into the split mode (1:10) for analysis using the GC-MS analysis protocol. After 2 min of operation in an oven set to 75 °C, the temperature was increased to 300 °C, at a rate of 15 °C/min, and maintained for 3 min. In order to collect the electron ionization (EI) mode, mass data were collected using an EI method with an ionization energy of 70 eV and a mass scan range of 50–600 m/z at an acquisition rate of 20 spectra/s. The ion source temperature and injector were set at 230 °C and 250 °C, respectively.

Kim W.S., Kim J, & Lee H.G. (2022). Identification of Potential Biomarkers and Metabolic Pathways of Different Levels of Heat Stress in Beef Calves. International Journal of Molecular Sciences, 23(17), 10155.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!