Rhodamine 123, l ‐NAME, and sodium dodecyl sulfate (SDS) were purchased from Wako (Tokyo, Japan). ISOGEN was purchased from Nippongene (Tokyo, Japan). DMEM, RIPA buffer, protease inhibitor cocktail, and β‐Actin antibody were purchased from Sigma (MO, USA). Fetal bovine serum (FBS) and house serum (HS) were purchased from Gibco (NY, USA). MTT or 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and l ‐NAME were purchased from Dojindo (Kumamoto, Japan). l ‐citrulline was supplied by Kyowa Hakko Bio Co., Ltd., Tokyo, Japan) while PGC‐1α (3G6) antibody was purchased from Cell Signaling Technology (Hertfordshire, UK). Glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) antibody (6C5) was purchased from Santa Cruz Biotechnology (CA, USA).
L name
Manufactured by Fujifilm
Sourced in Japan
L-NAME is a laboratory reagent used in biochemical research. It is a nitric oxide synthase inhibitor that can be utilized in experiments to study the role of nitric oxide in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Sodium dodecyl sulfate (sds), by Fujifilm (1 mentions)
Rhodamine 123, by Fujifilm (1 mentions)
L name, by Dojindo Laboratories (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody 6c5, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Isogen, by Nippon Gene (1 mentions)
Ly354740, by Santa Cruz Biotechnology (1 mentions)
Cay10441, by Cayman Chemical (1 mentions)
L name, by Cayman Chemical (1 mentions)
Myricetin, by Fujifilm (1 mentions)
Crystal violet, by Nacalai Tesque (1 mentions)
ε viniferin, by Fujifilm (1 mentions)
Anti sirt1, by Santa Cruz Biotechnology (1 mentions)
Anti mouse igg, by Santa Cruz Biotechnology (1 mentions)
Trans resveratrol, by Cayman Chemical (1 mentions)
Anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
6 protocols using l name
1
Analyzing Cellular Responses with Rhodamine 123 and L-NAME
2
Flavoprotein Fluorescence Imaging of Spinal Cord
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For flavoprotein fluorescence imaging, the spinal cord with the intact dura mater was exposed at the L5–L6 level, and 5 μl of 100 mM l ‐NAME (FUJIFILM Wako, Osaka, Japan), 2 mM NOR3 ((±)‐(E)‐4‐ethyl‐2‐[(E)‐hydroxyimino]‐5‐nitro‐3‐hexanamide, FUJIFILM Wako) or saline was intrathecally injected using a hypodermic needle of 30 gauge under direct visual control. When the effects of group II mGluRs on ischaemia‐induced NO production were examined, 5 μl of 10 nM LY354740 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), an agonist of group II mGluRs, was intrathecally injected 30 min before visualization of NO production. For behavioural experiments, 5 μl of 2 mM NOR3 or saline was blindly injected into the intrathecal space at the L5–L6 level of the non‐anesthetized mice. In the latter case, the position of the injection was verified by tail flick responses elicited by insertion of the needle (Hylden & Wilcox, 1980 ). In preliminary behavioural experiments, 5.0 μl of 1 mM NOR3 showed no significant effects.
3
Vasodilation and Calcium Signaling Assays
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Cilostazol (Pletal) was purchased from Otsuka pharmaceuticals (Tokyo, Japan). CAY‐10441 was purchased from Cayman Chemical (Michigan, USA). L‐NAME was purchased from WAKO (Tokyo, Japan). Pyr2 (BTP2; YM‐584893) was synthesized in Dr. Yasuo Mori's laboratory (Kyoto University) (Kiyonaka et al., 2009 (link)) and can be obtained from Tocris (Bristol, UK). Details of other materials and suppliers were provided in the specific sections.
4
Sennoside Effects in Hairless Mice
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Seven-week-old hairless Hos:HR-1 mice were obtained from Japan SLC, Inc. (Shizuoka, Japan) and housed in stainless steel wire mesh-bottomed cages with a constant temperature of 23℃±1℃, relative humidity of 50%±10%, and 12-hour light and dark cycles, as described previously3 (link). Mice were provided laboratory chow and water ad libitum. After an acclimation period of 1 week, the mice were randomly assigned to four groups (n=5/group), including the control, sennoside, sennoside+atropine, and sennoside+N(G)-nitro-L-arginine methyl ester (L-NAME) groups. Mice in the sennoside, sennoside+ atropine, and sennoside+L-NAME groups were given senna (10 mg/kg/day, postorally; Suzu Pharmaceutical Co., Ltd., Osaka, Japan) once a day for 21 days. Further, the sennoside+atropine and sennoside+L-NAME groups were intraperitoneally administered with atropine (1 mg/kg/day, once a day; Wako, Osaka, Japan) and L-NAME (20 mg/kg/day, once a day; Cayman Chemical, Ann Arbor, MI, USA), respectively. All mice were killed after 21 days of treatment with pentobarbital anesthesia. The animal protocol for this study was approved by the Animal Care Regulations Committee of the Suzuka University of Medical Science.
5
Clonogenic Assay for Compound Screening
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HeLa or A-204 cells were plated 200 cells/well in a 6-well plate and then allowed to attach overnight. The next day, compounds were placed in the plates at various concentrations. In the case of multi-compound treatment, 1 mM L-NAME or 6 mM myricetin (Wako) was added into the plate, and then ALESIA was. The HeLa or A-204 cells were incubated for 14 days or 28 days respectively, after which they were fixed and stained with 0.25 % crystal violet (Nacalai Tesque) and 25 % methanol in PBS at RT (20-26 C) for 30 min. The wells were washed with water once and dried at RT. The wells were imaged using the Cell 3 iMager CC-5000 (SCREEN Holdings, Kyoto, Japan). All images were scanned with a resolution of 2400 dpi. The colonies over 400 mm in diameter were defined as survival and the number of them was calculated by using Cell3iMager software version 2.4 (SCREEN Holdings).
6
Trans-resveratrol and Viniferin Bioactivity
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Trans-resveratrol was purchased from Cayman Chemical Co. (Tokyo, Japan) for cell experiments and from Tokyo Chemical Industry Co. (Tokyo, Japan) for the preparation of δ-viniferin, respectively. ε-Viniferin and L-NAME were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, Hanks' balanced salt solution (HBSS), SIRT1 inhibitor III (EX527), and peroxidase (type VII-A), and anti-heme oxygenase-1, anti-catalase, and anti-β-This website utilizes technologies such as cookies to enable essential site functionality, as well as for analytics, personalization, and targeted advertising. To learn more, view the following link: Privacy Policy actin antibodies were from Sigma Chemical Co. (Saint Louis, Missouri, USA). Diaminofluorescein-2 diacetate (DAF-2DA) was obtained from Sekisui Medical Co. (Tokyo, Japan). Anti-SIRT1, anti-mouse IgG, and antirabbit IgG antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, California). Anti-α-tubulin and zinc protoporphyrin-9 (ZnPPIX) were obtained from Calbiochem.
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