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Ezh2 and suz12 antibodies

Manufactured by Abcam
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EZH2 and SUZ12 are proteins that are part of the Polycomb Repressive Complex 2 (PRC2), which is involved in regulating gene expression. The EZH2 and SUZ12 antibodies can be used to detect and study these proteins in various experimental techniques.

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Lab products found in correlation

Ez chip kit, by Merck Group (3 mentions) H3 trimethyl lys 27 antibody, by Merck Group (3 mentions) Sybr green mix, by Takara Bio (3 mentions) E2f1 antibody, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

SUZ12 (22 citations) EZH2 and SUZ12 (2 citations)

3 protocols using ezh2 and suz12 antibodies

1

ChIP-qPCR Analysis of EZH2 and SUZ12

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The ChIP assays were performed using the EZ-CHIP KIT according to the manufacturer's instructions (Millipore, Billerica, MA, USA). EZH2 and SUZ12 antibodies were obtained from Abcam (Hercules, CA, USA). H3 trimethyl Lys 27 antibody was purchased from Millipore. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). The ChIP data were calculated as a percentage relative to the input DNA using the equation 2[Input Ct−Target Ct] × 0.1 × 100.
Su J., Zhang E., Han L., Yin D., Liu Z., He X., Zhang Y., Lin F., Lin Q., Mao P., Mao W, & Shen D. (2017). Long noncoding RNA BLACAT1 indicates a poor prognosis of colorectal cancer and affects cell proliferation by epigenetically silencing of p15. Cell Death & Disease, 8(3), e2665-.
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2

Chromatin Immunoprecipitation Protocol

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ChIP assays were performed using EZ-CHIP KIT according to the manufacturer, s instruction (Millipore, USA). EZH2 and SUZ12 antibodies were obtained from Abcam. E2F1 was from Cell Signaling Technology. H3 trimethyl Lys 27 antibody was from Millipore. The ChIP primer sequences were listed in Supplementary Table S1. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). ChIP data was calculated as a percentage relative to the input DNA by the equation 2[Input Ct- Target Ct] × 0.1×100.
Zhang E.B., Kong R., Yin D.D., You L.H., Sun M., Han L., Xu T.P., Xia R., Yang J.S., De W, & Chen J.F. (2014). Long noncoding RNA ANRIL indicates a poor prognosis of gastric cancer and promotes tumor growth by epigenetically silencing of miR-99a/miR-449a. Oncotarget, 5(8), 2276-2292.
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3

Chromatin Immunoprecipitation Assay Protocol

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ChIP assays were performed using the EZ-CHIP KIT according to the manufacturer's instructions (Millipore, USA). EZH2 and SUZ12 antibodies were obtained from Abcam. E2F1 antibody was obtained from Cell Signaling Technology. H3 trimethyl Lys 27 antibody was obtained from Millipore. The ChIP primer sequences are listed in Supplementary Table S1. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). ChIP data were calculated as a percentage relative to the input DNA by the equation 2[Input Ct- Target Ct] × 0.1×100.
Zhang E., Yin D., Han L., He X., Si X., Chen W., Xia R., Xu T., Gu D., De W., Guo R., Xu Z, & Chen J. (2015). E2F1-induced upregulation of long noncoding RNA LINC00668 predicts a poor prognosis of gastric cancer and promotes cell proliferation through epigenetically silencing of CKIs. Oncotarget, 7(17), 23212-23226.
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