PHH-derived organoids were seeded on a 10 mg/mL HYDROX-coated 96-well plate. After 14 days of the HYDROX culture, Org-HYDROX were cultured with organoid expansion medium containing different concentrations of acetaminophen (Wako), troglitazone (Wako) and amiodaron (FUJIFILM Wako Pure Chemical) for 7 days. As a control group, PHHs (lot OHO; Celsis) cultured with hepatocyte culture medium (HCM; Lonza) for 48 h after plating on a 96-well plate were exposed to different concentrations of acetaminophen (Wako), troglitazone (Wako) and amiodaron (FUJIFILM Wako Pure Chemical) for 7 days. Cell viability was evaluated using a Cell Counting Kit-8 (Dojindo Laboratories) based on a WST-8 assay according to the manufacturer's instructions. The absorbance at 450 nm was determined by a multiplate reader (Bio-Rad). Cell viability was calculated as a percentage of the control (DMSO-treated group) viability, which was taken as 100%.
Tong Y., Ueyama-Toba Y., Yokota J., Matsui H., Kanai M, & Mizuguchi H. (2024). Efficient hepatocyte differentiation of primary human hepatocyte-derived organoids using three dimensional nanofibers (HYDROX) and their possible application in hepatotoxicity research. Scientific Reports, 14, 10846.
+ Open protocol