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Disodium tartrate

Manufactured by Merck Group
Sourced in Germany

Disodium tartrate is a chemical compound used as a reagent in various laboratory applications. It is a salt of tartaric acid, with the molecular formula Na2C4H4O6. Disodium tartrate is a white, crystalline powder that is soluble in water and commonly used in analytical procedures, buffer solutions, and as a complexing agent in titrations.

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2 protocols using disodium tartrate

1

Synthesis of Luminescent Coordination Polymers

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Silver
nitrate (AgNO3), calcium chloride dihydrate (CaCl2·2H2O), sodium nitrate (NaNO3), potassium
chloride
(KCl), manganese chloride (MnCl2), copper II chloride dihydrate
(CuCl2·2H2O), magnesium chloride anhydrous
(MgCl2), aluminum chloride (AlCl3), iron III
chloride (FeCl3), dysprosium III chloride hexahydrate (DyCl3·6H2O), sodium citrate tribasic tetrahydrate
(HOC(COONa) (CH2COONa)2·4H2O),
hydrogen peroxide (H2O2), terbium III chloride
hexahydrate (TbCl3·6H2O), ytterbium III
chloride hexahydrate (YbCl3·6H2O), holmium
III chloride hexahydrate (HoCl3 ·6H2O),
lead chloride (PbCl2), sodium carbonate anhydrous (Na2CO3), sodium phosphate (Na3PO4), sodium sulfate (Na2SO4), sodium fluoride
(NaF), sodium acetate (CH3COONa), disodium tartrate, disodium
succinate, disodium malate, CTAB, polyethylene glycol 1500, and dodecanoic
acid were purchased from Sigma-Aldrich Company (South Korea). Fumaric
acid, p-toluene sulfonic acid (PTSA), and 3-(4-hydroxyphenyl)propanoic
acid were purchased from Merck (Germany). All solvents and chemicals
were used as received without purification. DI water filtered to 18
MΩ·cm was used in all experiments.
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2

Quantification of Multinucleated Osteoclasts

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0.3 mg/mL Fast Red Violet LB and 0.1 mg/mL naphthol AS-MX phosphate disodium salt (both Sigma-Aldrich, Munich, Germany) were dissolved in 100 mM sodium acetate buffer pH 6.1 containing 50 mM disodium tartrate (Sigma-Aldrich, Munich, Germany). Cells were fixed with 4% formaldehyde and incubated for 10 min at 37 °C with the staining solution. After washing with PBS, cell nuclei were stained for 10 min using Mayer’s Haemalum solution (AppliChem, Darmstadt, Germany) followed by rinsing in tap water. Stained samples were imaged using a Biorevo BZ-9000 (Keyence, Neu-Isenburg, Germany). For counting of multinucleated TRAP positive cells, three wells (48 well size) per culture condition and PBMC donor were analysed with each 4 images (20× magnification) for each sample. The cells were counted manually using the cell counter plugin of Fiji.
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