Mn and PDI of P(SPMA0.5-r-MMA0.5) were acquired by GPC. The polyelectrolyte was dissolved in DI water (3 mg mL−1), filtered through a sterilized filter (0.22 µm pore size; Ministart PES, Sartorius), then injected into a column (PL aquagel-OH 40, Agilent) for sample resolving at a flow rate of 1 mL min−1. A refractive index detector in the chromatography system (Agilent 1100 Series) was employed to sample the retention time. A standard kit (EasiVial PEG/PEO 2 mL, Agilent) was used to calibrate the relationship between retention time and molecular weight. The molecular structure of P(SPMA0.5-r-MMA0.5) was examined by 1H NMR spectroscopy (DPX 400, Bruker).
Pl aquagel oh 40
Manufactured by Agilent Technologies
Sourced in United States
The PL aquagel-OH 40 is a size exclusion chromatography column designed for the analysis of aqueous samples. It is intended for the separation and analysis of water-soluble polymers, proteins, and other macromolecules.
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3 protocols using pl aquagel oh 40
1
Molecular Characterization of P(SPMA-r-MMA) Polyelectrolyte
2
GPC Analysis of Polymer Molecular Weights
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Polymer molecular weights (Mn, Mw) and dispersity (Đ) were determined by GPC using an Agilent 1260 infinity II GPC system equipped with an Agilent 1260 infinity RI detector, and either a Superpose 6 increase 10/300 GL (GE healthcare) column (bimodal pDMAPMA, pCB with and without butylamine), Superose 6 increase 10/300 GL and HiLoad 16/600 Superdex 200 pg (GE healthcare) columns in series (trimodal pDMAPMA), or PL aquagel-OH 30 and PL aquagel-OH 40 (Agilent) columns in series (monomodal pCB) with PBS running buffer at 30 °C. Columns were calibrated using polyethylene glycol (PEG) standards (Mn of 3000 to 60 000 Da).
GPC chromatograms of bimodal pCB were deconvolved with Microsoft Excel assuming two peaks fit as normal Gaussian distributions using the included generalized reduced gradient (GRG) nonlinear algorithm. The ratio of high to low molecular weight polymers in bimodal distributions were calculated as the relative ratio of the area of these Gaussian distributions.
GPC chromatograms of bimodal pCB were deconvolved with Microsoft Excel assuming two peaks fit as normal Gaussian distributions using the included generalized reduced gradient (GRG) nonlinear algorithm. The ratio of high to low molecular weight polymers in bimodal distributions were calculated as the relative ratio of the area of these Gaussian distributions.
3
Chitosan Peroxide Modification
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Chitosan (15 g) was dissolved in 2% glacial acetic acid solution, and 3% H2O2 was dropwise added, followed by incubation at 60°C for 0, 0.5, 1, and 2 h, respectively. The reaction was terminated with NaOH solution and the pH value of the solution was adjusted to 7. Precipitation was done by adding a large amount of ethanol to wash the samples by suction filtering. The prepared samples were freeze-dried and labeled as CS0, CS1, CS2, and CS3. The molecular weight of the samples was measured using water-soluble gel permeation column PL aquagel-OH 40 (Agilent, Palo Alto, USA). The sample was dissolved in a sodium acetate buffer (pH = 4.8) at a concentration of 0.1%.
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