Paclitaxel

Manufactured by GoldBio

Paclitaxel is a chemical compound that is widely used in the field of biotechnology and pharmaceutical research. It is a naturally occurring substance derived from the bark of the Pacific yew tree. Paclitaxel is a potent antineoplastic agent, which means it has the ability to inhibit the growth and proliferation of cancer cells. It functions by disrupting the normal cell division process, leading to cell death. Paclitaxel is commonly used in the development and testing of new cancer treatments and therapies.

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Lab products found in correlation

Paclitaxel, by Beckman Coulter (2 mentions) Airfuge, by Beckman Coulter (2 mentions)

4 protocols using paclitaxel

Microtubule Polymerization and Stabilization

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A polymerization mixture was prepared with BRB80 + 32 μM tubulin + 1 mM GTP + 4 mM MgCl₂ + 5% DMSO. The mixture was incubated on ice for 5 min, followed by incubation at 37°C for 30 min. The polymerized microtubules were diluted into prewarmed BRB80 + 10 μM paclitaxel, centrifuged at 110,000 rpm (199,000 × g) in an Airfuge (Beckman-Coulter), and resuspended in BRB80 + 10 μM paclitaxel (99.5+%, GoldBio).

McAlear T.S, & Bechstedt S. (2022). The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules. eLife, 11, e72202.

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Paclitaxel-Induced Neuropathic Pain in Rats

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Rats were given paclitaxel (Cat# P-925–1, Goldbio, Olivette, MO) based on the protocol described by Polomano et al. [63 (link)]. In brief, pharmaceutical-grade paclitaxel (Taxol) was re-suspended at a concentration of 2 mg/ml in 30% 1:1 Cremophor EL: ethanol, 70 % Saline and given to the rats at 2 mg/kg intraperitoneally (i.p.) every other day for a total of 4 injections (days 0, 2, 4, and 6), resulting in a final cumulative dose of 8 mg/kg. No abnormal spontaneous behavioral changes in the rats were noted during or after the treatment. Animals developed mechanical hyperalgesia within 10 days after the first paclitaxel injection.

Bellampalli S.S., Ji Y., Moutal A., Cai S., Kithsiri Wijeratne E.M., Gandini M.A., Yu J., Chefdeville A., Dorame A., Chew L.A., Madura C.L., Luo S., Molnar G., Khanna M., Streicher J.M., Zamponi G.W., Leslie Gunatilaka A.A, & Khanna R. (2019). Betulinic acid, derived from the desert lavender Hyptis emoryi, attenuates paclitaxel-, HIV-, and nerve injury-associated peripheral sensory neuropathy via block of N- and T-type calcium channels. Pain, 160(1), 117-135.

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Paclitaxel-Induced Neuropathy Protocol

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Neuropathy with paclitaxel (Cat# P-925–1, Goldbio, Olivette, MO)
was induced with a final cumulative dose of 8 mg/kg using methods described
earlier [60 (link)].

Cai S., Tuohy P., Ma C., Kitamura N., Gomez K., Zhou Y., Ran D., Bellampalli S.S., Yu J., Luo S., Dorame A., Pham N.Y., Molnar G., Streicher J.M., Patek M., Perez-Miller S., Moutal A., Wang J, & Khanna R. (2020). A modulator of the low-voltage activated T-type calcium channel that reverses HIV glycoprotein 120-, paclitaxel-, and spinal nerve ligation-induced peripheral neuropathies. Pain, 161(11), 2551-2570.

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Microtubule Curvature Analysis with CKAP2

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A polymerization mixture was prepared with BRB80 + 32 μM tubulin + 1 mM GTP + 4 mM MgCl2 + 5% DMSO. The mixture was incubated on ice for 5 min, followed by incubation at 37°C for 30 min. The polymerized microtubules were diluted into prewarmed BRB80 + 10 μM paclitaxel, centrifuged at 110,000 rpm (199,000 × g) in an Airfuge (Beckman-Coulter), and resuspended in BRB80 + 10 μM paclitaxel (99.5+%, GoldBio) Curvature Recognition TAMRA labelled paclitaxel microtubules were introduced into flow channels (Bechstedt et al., 2014) . 5 nM CKAP2-mNG in imaging buffer with 10 μM paclitaxel without GTP was introduced into the channel. Quantification of the microtubule curvature, κ, and CKAP2-mNG signal on microtubules was analyzed using Kappa (Mary and Brouhard, 2019) .

McAlear T.S., & Bechstedt S. (2021). The mitotic spindle protein CKAP2 potently increases formation and stability of microtubules.

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