Bipolar stimulation was performed on 8 electrode pairs around the nerve circumference. Biphasic cathodic-first pulses of 10–400 μA (steps of 10 μA) with a pulse duration of 300 μs were generated by an IZ2 stimulator (Tucker-Davis Technologies) and transmitted to the selected cuff electrode pairs through a custom PCB. The EMG signals were acquired using a PZ5 NeuroDigitizer Amplifier (Tucker-Davis Technologies). Signals were amplified (× 1000) and bandpass filtered at 10–250 Hz, with an additional bandstop at 50 Hz. Data processing was performed in Matlab 2019b. Maximal EMG amplitude for each muscle was obtained considering all sessions (sciatic and tibial stimulation), while normalized amplitude and selectivity indexes were computed as previously described above, with EMG threshold in this case set to 0.4, similarly to previous work (Strauss et al. 2020 (link)).
Iz2 stimulator
Manufactured by Tucker-Davis Technologies
The IZ2 stimulator is a versatile instrument designed to deliver electrical stimulation for various research and clinical applications. It features programmable voltage and current stimulation capabilities, enabling precise control over the stimulation parameters. The IZ2 stimulator is a core component in experimental setups where controlled electrical stimulation is required.
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Lab products found in correlation
3 protocols using iz2 stimulator
1
Selective Nerve Stimulation: EMG Insights
2
Theta-Burst Stimulation in Pilocarpine-Induced Epilepsy
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Rats were counterbalanced into treatment groups based on a combination of average weight from post-pilocarpine day 1–3 (PPD1–3; Figure 1B ) and the number of cycles of status epilepticus in the first 2 h after pilocarpine injection (Figure 1C ). Control animals included vehicle controls for pilocarpine with no stimulation (Vehicle; n = 14) and pilocarpine-treated rats with no stimulation (Pilo; n = 13) rats. Experimental pilocarpine groups received either 7.7 Hz theta (Fixed; n = 12) or theta-burst stimulation (Burst; n = 13). On PPD4–16, animals were placed in a Plexiglas box (28 × 28 × 30 cm), and bipolar MSN stimulation was applied (IZ2 Stimulator; Tucker Davis Technologies) for 30 min/day. Based on previous studies, stimulation parameters were as follows: (Fixed) 7.7 Hz at 80 μA with 1 ms pulse-width (24 (link), 29 (link)–33 (link)); (Burst) 50 ms trains of 200 Hz, 5 trains/s, 60 μA, 100 μs pulse-width (34 (link)).
3
Waveform Effects on Neural Stimulation
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Stimulation was delivered via TDT RZ6D system controlling an IZ2 stimulator (Tucker-Davis Technologies, Alachua, FL) at 10 or 100 Hz using charge-balanced biphasic cathodal-or anodal-leading waveforms (Table 1). A monopolar setup was used to better isolate the waveform specific effects at a single electrode location (39) . The asymmetry of the waveforms was defined by the ratio of the leading phase to the return phase(40) (Eqn. 1) and is comparable to waveforms that can be used clinically (4, 23) . Stimulation trials involved a 30s baseline, 30s stimulation, and 20s post-stimulation period. All 8 stimulation waveforms were delivered in random order at 10 or 100 Hz for each session with a minimum of 5 minutes between trials.
All waveforms deliver 2.5nC/phase (0.36mC/cm 2 ) which is within safety recommendations of microelectrodes (41, 42) . Waveforms in figures are represented by the leading polarity with a subscript of the asymmetry index (e.g. C0 represents symmetric cathodal-leading).
All waveforms deliver 2.5nC/phase (0.36mC/cm 2 ) which is within safety recommendations of microelectrodes (41, 42) . Waveforms in figures are represented by the leading polarity with a subscript of the asymmetry index (e.g. C0 represents symmetric cathodal-leading).
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