For the Cu system, Cu foils with a thickness of 171 μm (Online Metals) were used as the cathode and anode electrodes and were embedded into the side channels. The Cu electrode had a surface area of 3.42 × 10−3 cm2. The electrode edges were polished by 6000 grit sandpaper (Micro-Surface Finishing Products). Electrolytes containing 0.02, 0.1, and 0.2 M CuSO4 were prepared using CuSO4 · 5H2O (Avantor Performance Materials), dissolved in deionized (DI) water. The electrochemical deposition for Cu was conducted for 60 s, under the applied voltage of 1.5, 2.0, 2.5, and 3.0 V, respectively. The Cu electrochemical deposition at constant current density was performed for 200 s. For the streamline tracking, the electrolyte solution was seeded with 1-μm negative carboxylate microspheres (Polysciences) and monitored under constant current density for 110 s. A nonionic surfactant (0.1 weight %), Tween 20, was added to the solution to avoid particle aggregation. The final particle concentration of the applied electrolyte solution was 6.83 × 107 particles/ml.
Cuso4 5h2o
Manufactured by Avantor
Sourced in United States
CuSO4·5H2O is a chemical compound that consists of copper sulfate and five water molecules. It is a blue, crystalline solid that is widely used in various industries and applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Avantor (3 mentions)
H2so4, by Avantor (3 mentions)
Sodium chloride (nacl), by Avantor (2 mentions)
Sodium hydroxide (naoh), by Avantor (2 mentions)
Hydrogen peroxide, by Avantor (2 mentions)
Thiourea, by Avantor (2 mentions)
Potassium ferricyanide, by Avantor (2 mentions)
Feso4, by Avantor (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Dinitrophenyl hydrazine dnph, by Thermo Fisher Scientific (2 mentions)
No 1 filter paper, by Cytiva (1 mentions)
Whatman, by Cytiva (1 mentions)
Agno3, by Avantor (1 mentions)
Na2co3, by Avantor (1 mentions)
Hexadecyltrimethylammonium bromide, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Formaldehyde, by Avantor (1 mentions)
Urea, by Avantor (1 mentions)
Tetramethylammonium hydroxide pentahydrate, by Merck Group (1 mentions)
Dodecyltrimethylammonium chloride, by Merck Group (1 mentions)
11 protocols using cuso4 5h2o
1
Electrochemical Copper Deposition and Characterization
2
Biosorption of Zn(II) and Cu(II) by Fucus vesiculosus
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The biosorption of Zn(II) and Cu(II) ions by Fucus vesiculosus was carried out in a stirred tank reactor (Biotron LiFlus LiFlus GX) at room temperature for 2 hours (3 grams of the biomass were mixed with 3 L of metal ions solution). The content of the biomass in the solution was 1 g of dry mass (d.m.) L -1 according to previous studies (Michalak and Chojnacka, 2010) . The stock solution of Zn(II) and Cu(II) ions (300 mg L -1 ) was prepared by dissolving appropriate amounts of ZnSO4•7H2O and CuSO4•5H2O (Avantor Performance Materials Poland S.A.) in distilled water. pH of initial solutions was adjusted to 5 with 0.1 M HCl or/and NaOH (with the use of pH meter Mettler Toledo equipped with an electrode InLab413 with compensation of temperature, SevenMulti, Switzerland). The solution was then filtered using Whatman No.1 filter paper and the concentration of metal ions in the solution before and after biosorption was determined by ICP-OES (Inductively Coupled Plasma-Optical Emission Spectrometry). The biosorption capacity (calculated as a difference of metal ions concentration in the solution before and after biosorption process) of Fucus vesiculosus towards Cu(II) ions was 45.0 mg g -1 of d.m. and Zn(II) ions -10.3 mg g -1 . The obtained enriched biomass was air-dried and used later in germination tests as a fertilizing material.
3
Production of SAP Fiber from Spruce Pulp
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A spruce sulfite pulp with DP 590 from Domsjö fabriker AB, Domsjö/Sweden, N-methylmorpholine-N-oxide of BASF/Leverkusen/Germany as a 50 wt.% aqueous solution, and polyacrylate from Evonik/Essen/Germany were used to produce the SAP fiber. SAP is partly crosslinked with NaOH, containing 3.43 mmol COO−/g (Titration) and 19.9% Na (ICP-OES). Glucose, AgNO3, NaCl, CuSO4·5H2O, and Na2CO3 were purchased from VWR International GmbH/Darmstadt/Germany as p.a. products.
4
Cellulose Dissolution in LiOH/Urea Solvent
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The chelating agents, ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA), were supplied by Sigma-Aldrich (Stockholm, Sweden). The chelating surfactant 2-dodecyldiethylenetriaminepentaacetic acid (C12-DTPA) was delivered by Syntagon AB (Stockholm, Sweden) [16 (link)]. The chemicals hexadecyltrimethylammonium bromide (CTAB), dodecyltrimethylammonium chloride (DoTAC), sodium dodecyl sulfate (SDS), dimethyldodecylamine-N-oxide (DDAO), trimethylamine N-oxide dehydrate and tetramethylammonium hydroxide pentahydrate were of analytical grade, obtained from Sigma-Aldrich (Stockholm, Sweden), and used without further purification. CuSO4·5H2O, NaOH, LiOH·H2O, urea, and formaldehyde (CH2O, 36 wt % solution) were supplied by VWR International (Umeå, Sweden). Cellulose in the form of dissolving pulp with viscosity of 450 mL g−1 was supplied by Domsjö Fabriker (Örnsköldsvik, Sweden). The cellulose was ground and dissolved directly in aqueous 4.6 wt % LiOH/15 wt % urea solution precooled to –12 °C to prepare a transparent 1 wt % cellulose solution [17 (link)]. The water used for the preparation of samples was of Milli-Q grade.
5
Clickable Glycoconjugate Synthesis Protocol
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Bacto-tryptone and yeast extract were obtained from Biokar Diagnostics (FR). LB medium was purchased from Sigma-Aldrich (FR). Ampicillin was purchased from Eurobio (FR). Glycerol and isopropyl β-D-thiogalactopyranoside (IPTG) were purchased from Euromedex (FR).
Glycidyl propargyl ether was obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, FR).
Glacial acetic acid, Trizma® and Hexafluoroisopropanol (HFIP) were obtained from Sigma-Aldrich (FR). Deionized water (18 MΩ-cm) was obtained by using a Millipore Milli-Q Biocel A10 purification unit. Cuprisorb was purchased from Seachem. Ethanol (96.0%, EtOH), mEthanol (98.5%, MeOH) and acetonitrile (99.9%, ACN) were obtained from VWR international. NaCl (99%) was purchased from Alfa Aesar (FR). Azide monosaccharides (β-Dgalactopyranosyl azide, Gal-N 3 ; and β-D-glucopyranosyl azide (Glu-N 3 ) were obtained from Carbosynth (UK). Ammonium Acetate and Ammonium pyrrolidinedithiocarbamate, APDC, were purchased from Fisher Scientific (FR). RCA 120 and RCA 120 -Fluorescein were purchased from Eurobio (FR). Human serum from human male AB plasma, USA origin, sterile-filtered, was used as received from Sigma-Aldrich (FR). N,N,N′,N′′,N′′-pentamethyldiethylenetriamine (PMDETA) was purchased from Sigma-Aldrich (FR). CuSO 4 •5H 2 O was obtained from VWR (FR). Sodium ascorbate was obtained from Fisher Scientific (FR).
Glycidyl propargyl ether was obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, FR).
Glacial acetic acid, Trizma® and Hexafluoroisopropanol (HFIP) were obtained from Sigma-Aldrich (FR). Deionized water (18 MΩ-cm) was obtained by using a Millipore Milli-Q Biocel A10 purification unit. Cuprisorb was purchased from Seachem. Ethanol (96.0%, EtOH), mEthanol (98.5%, MeOH) and acetonitrile (99.9%, ACN) were obtained from VWR international. NaCl (99%) was purchased from Alfa Aesar (FR). Azide monosaccharides (β-Dgalactopyranosyl azide, Gal-N 3 ; and β-D-glucopyranosyl azide (Glu-N 3 ) were obtained from Carbosynth (UK). Ammonium Acetate and Ammonium pyrrolidinedithiocarbamate, APDC, were purchased from Fisher Scientific (FR). RCA 120 and RCA 120 -Fluorescein were purchased from Eurobio (FR). Human serum from human male AB plasma, USA origin, sterile-filtered, was used as received from Sigma-Aldrich (FR). N,N,N′,N′′,N′′-pentamethyldiethylenetriamine (PMDETA) was purchased from Sigma-Aldrich (FR). CuSO 4 •5H 2 O was obtained from VWR (FR). Sodium ascorbate was obtained from Fisher Scientific (FR).
6
Neurospora crassa Growth and Maintenance
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The experimental fungus used in this study was Neurospora crassa (FGSC: 2489, Fungal Genetics Stock Centre (FGSC), Kansas, USA). It was routinely maintained on malt extract agar (MEA, Lab M limited, Bury, Lancashire, UK) in 90-mm diameter Petri dishes and grown at 25 °C in the dark. A urea-modified AP1 medium was used as the liquid media consisting of 2% (w/v) d -glucose (Merck, Readington Township, NJ, USA), 40 mM urea (Sigma-Aldrich, St. Louis, MO, USA), 4 mM K2HPO4∙3H2O (Sigma-Aldrich, USA), 0.8 mM MgSO4∙7H2O (Sigma-Aldrich, USA), 0.2 mM CaCl2∙6H2O (Sigma-Aldrich, USA), 1.7 mM NaCl (Sigma-Aldrich, USA), 9 × 10−3 mM FeCl3∙6H2O (Sigma-Aldrich, USA) and trace metals 0.014 mM ZnSO4∙7H2O (VWR, Radnor, PA, USA), 0.018 mM MnSO4∙4H2O (Sigma-Aldrich, USA) and 1.6 × 10−3 mM CuSO4∙5H2O (VWR, USA) (Li et al. 2014 (link)). After 3 days growth in the full AP1 medium, fungal biomass was collected and washed twice in sterile MilliQ water after centrifugation (× 4000g, 30 min), and continued to be incubated in a sterile phosphate-free AP1 medium for 12 days. The initial pH of AP1 medium was adjusted to pH 5.5 using 1 M HCl after autoclaving. All experiments were conducted at least in triplicate.
7
Analytical Procedure for Chitin Analysis
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Petroleum ether (bp. 40 -60 °C, technical), H2SO4 (95 %, technical), K2SO4, CuSO4.5H2O, NaOH, and H3BO3 were all supplied by VWR Chemicals. Tin pressed capsules (8 mm 𝗑 5 mm, standard clean) and all reagents required for the Dumas method were purchased from OEA Laboratories Ltd. Helium was supplied by Nippon Gases. The analytical standard of acetanilide was provided by Thermo Fisher. Chitin (practical grade), HCl (37.0 %), 9-fluorenylmethyl chloroformate (FMOC-Cl), D-glucosamine hydrochloride (analytical standard), and amino acid standards were purchased from Sigma Aldrich. Syringe filters (Chromafil PET 0.20 µm) and Soxhlet thimbles were supplied by Macherey-Nagel. ULC-MS grade acetonitrile and formic acid were bought from Biosolve. The derivatisation reagent for amino acid analyses (6aminoquinolyl-N-hydroxysuccinimidyl carbamate, AQC) was supplied by Waters Co.
Ultrapure water was obtained through the Arium Pro® UV from Sartorius. All reagents were of analytical grade unless specified otherwise.
Ultrapure water was obtained through the Arium Pro® UV from Sartorius. All reagents were of analytical grade unless specified otherwise.
8
Colorimetric Reagent Synthesis and Characterization
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CR dye (C32H22N6Na2O6S2) and
urea were procured from Techno Pharmchem, Haryana, India. CuSO4·5H2O was procured from Chadwell Heath, Essex,
England, and BDH Chemicals Ltd. Sigma-Aldrich Co., USA, supplied sodium
hydroxide, and Al2(NO3)3·9H2O was obtained from Panreac Quimica SAU, Spain. Scharlab S.L.,
Spain, supplied sodium dodecyl sulfonate (SDS). MnCl2·4H2O, K2Cr2O7,, and
ammonia solution were supplied by BDH Chemicals Ltd., Poole, England.
urea were procured from Techno Pharmchem, Haryana, India. CuSO4·5H2O was procured from Chadwell Heath, Essex,
England, and BDH Chemicals Ltd. Sigma-Aldrich Co., USA, supplied sodium
hydroxide, and Al2(NO3)3·9H2O was obtained from Panreac Quimica SAU, Spain. Scharlab S.L.,
Spain, supplied sodium dodecyl sulfonate (SDS). MnCl2·4H2O, K2Cr2O7,, and
ammonia solution were supplied by BDH Chemicals Ltd., Poole, England.
9
Antioxidant and Anti-inflammatory Assays
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Trichloroacetic acid (TCA), gallic acid, De Man, Rogosa and Sharpe (MRS) broth, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and Folin–Ciocalteu's reagent were procured from Sigma‐Aldrich, Inc. Dinitrophenyl hydrazine (DNPH) was from ACROS Organics; hydrogen peroxide, methanol, and FeCl3 were purchased from BDH Chemicals Ltd.; and thiourea, CuSO4.5H2O, H2SO4, sodium carbonate, AlCl3, potassium acetate, Tris–HCl buffer, FeSO4, potassium ferricyanide, and ferric chloride were of analytical grade while the water was glass‐distilled. Lipopolysaccharide (LPS), sodium nitrite (NaNO2), and Griess reagent (0.1% N‐(1‐naphthyl)ethylenediamine dihydrochloride, 1% sulfanilamide in 5% phosphoric acid) were all prepared in‐house from reagents purchased from Sigma‐Aldrich Korea. Viscozyme, cellulase, amylase, and protease were supplied by Erom Company Limited.
10
Antioxidant and Reducing Potential Assays
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Chemicals and reagents used such as thiobarbituric acid (TBA), 1,10-phenanthroline, deoxyribose, gallic acid, and Folin-Ciocalteau's reagent were procured from Sigma-Aldrich, Inc. (St Louis, MO), trichloroacetic acid (TCA) was sourced from Sigma-Aldrich, Chemie GmbH (Steinheim, Germany), dinitrophenylhydrazine (DNPH) was sourced from ACROS Organics (New Jersey, USA), and hydrogen peroxide, methanol, acetic acid, thiourea, CuSO4·5H2O, H2SO4, HCl, sodium carbonate, AlCl3, potassium acetate, Tris-HCl buffer, sodium dodecyl sulphate, FeSO4, potassium ferricyanide, and ferric chloride were sourced from BDH Chemicals Ltd. (Poole, England), while the water was glass distilled.
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