Rabbit anti apaf 1

Western blots were performed as described [38] (link). Mouse monoclonal anti-Caspase 8 (Sigma), mouse monoclonal anti-Caspase 9 (Sigma), rabbit anti-Caspase 3 (Sigma), rat monoclonal anti-Caspase 12 (Sigma), rabbit polyclonal antibody against Bim (amino acids 4–195 of Bim_EL form), rabbit anti-Cytochrome C (Cell Signaling), goat polyconal anti-HP1 (Santa Cruz), rabbit anti-CoxIV (Cell Signaling), rabbit anti-phospho-JNK (Thr183/Tyr185) (81E11) (Cell Signaling), rabbit anti-JNK (Cell Signaling), mouse monoclonal anti-CHOP (L63F7) (Cell Signaling), rabbit anti-Apaf-1 (Cell Signaling), rabbit anti-Bcl2 (Cell Signaling), rabbit polyclonal anti-ATF-6 (Santa Cruz), rabbit anti-Bax (Cell Signaling) were all used at a 1:1000 dilution for western blots.

Human NPC cell lines (CNE-2 and 5-8F) were purchased from cell bank of the Chinese Academy of Sciences (Shanghai, China). RPMI 1,640 medium and fetal bovine serum (FBS) was purchased from Gibco (Logan, UT, USA). Rabbit anti-caspase 3 (#9,662), Rabbit anti-α-tubulin (#21,44S), Rabbit anti-Apaf-1 (#89,69S), Rabbit anti-caspase 9 (#95,08S), Rabbit anti-Acetyl-p53 (Lys382) (#25,25S), and Rabbit anti-SIRT1 (#2,493) were purchased from Cell Signaling Technology (Boston, MA, USA). EGCG (purity > 95%) was purchased from Sigma Aldrich and dissolved in distilled water at 100 μM, and stored at −20°C until dilution before use. The cell counting kit 8 (CCK8) was purchased from DOJINDO (Japan). The terminal deoxynucleotidyl transferase (TDT)-mediated dUTP nick end labeling (TUNEL) assay kit was purchased from the Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China). The BCA protein assay kit was purchased from Thermo Fisher Scientific (Chicago, IL). Super signal chemiluminescent substrate (ECL) was obtained from Thermo Scientific (Waltham, MA, USA).