Eos rebel t2i digital camera

Images were taken using DIC optics on an AxioImager M2 microscope (Zeiss) with an 18.0-megapixel EOS Rebel T2i digital camera (Canon) for WMISH animals or an AxioCam MRm rev.3 camera (Zeiss) with Zen Blue software (Zeiss) for antibody-labeled animals or live imaging of mVenus. DiI-labeled animals were imaged using a Zeiss Apotome.2 to produce optical sections. Animals for confocal laser scanning microscopy were imaged using a TCS SP5- X (Leica). DIC images taken at different focal planes were merged with Helicon focus 7 (Helicon). Different channels and z-stacks of fluorescent images were merged using Zen Blue (Zeiss). WHISH images were edited for contrast and brightness using Adobe Photoshop CC (Adobe). Figure panels were assembled with Adobe Illustrator CC (Adobe).

WMISH images were taken using DIC optics on a Zeiss M2 compound microscope coupled with an 18.0 megapixel EOS Rebel T2i digital camera (Canon). All animals labeled by immunohistochemistry were imaged by confocal laser scanning microscopy. Animals labeled with anti-FMRF were imaged using an LSM 710 (Zeiss). Stage 4 – 6 larvae and juveniles labeled with anti-serotonin and anti-acetylated-α-tubulin were imaged using an LSM 510 (Zeiss). Stage 7 – 9 larvae labeled with anti-serotonin and anti-acetylated-α-tubulin were imaged using a TCS SP5-X (Leica). Z-stack projections were generated using ImageJ (NIH). Some WMISH images were joined into a composite image using Helicon Focus 6.2.0 (Helicon Soft). Images (confocal and WMISH) were edited using Photoshop CS4 and figure panels were constructed using Illustrator CS4 (Adobe Systems Inc.).