Penicillin streptomycin solution 100x

Manufactured by Euroclone

Sourced in Italy

Penicillin–streptomycin solution 100X is a commonly used antibiotic solution used in cell culture applications. It contains a combination of the antibiotics penicillin and streptomycin at a concentration 100 times the standard strength.

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Lab products found in correlation

L glutamine 100x 200 mm, by Euroclone (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Euroclone (2 mentions) Silac dmem, by Thermo Fisher Scientific (1 mentions) L arginine arg0, by Merck Group (1 mentions) L lysine lys0, by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Thymidine, by Merck Group (1 mentions) Rpmi 1640 medium without l glutamine with phenol red, by Euroclone (1 mentions) Hepes, by Merck Group (1 mentions) Mycoalert mycoplasma testing kit, by Lonza (1 mentions) L glutamine 100x, by Euroclone (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Tryptone, by Merck Group (1 mentions) Yeast extract, by Merck Group (1 mentions) Lympholyte h, by Euroclone (1 mentions) Sharpmass 6 mw marker, by Euroclone (1 mentions) Protease inhibitor cocktail 100x, by Cell Signaling Technology (1 mentions) Ivacaftor vx 770, by Merck Group (1 mentions) Amersham protran premium 0.45 μm nitrocellulose, by GE Healthcare (1 mentions)

Spelling variants of this product

Streptomycin (432 citations) Penicillin (428 citations) Penicillin/streptomycin (380 citations) Penicillin/streptomycin solution (24 citations) Penicillin/streptomycin 100X (6 citations)

6 protocols using penicillin streptomycin solution 100x

Culturing Human Lung and Cancer Cells

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The human lung fibroblast cell line MRC-5 was supply by (LGC Standard-ATCC CCL171). MRC-5 cells were maintained in Eagle’s Minimum Essential Medium (EMEM) ATCC 30-2003, supplemented with 15% FBS (Euroclone), 1% penicillin-streptomycin solution 100X (Euroclone) and 1% non-essential-amino acid (Euroclone).
For the human cancer cell line HeLa (LGC Standard-ATCC CCL-2), cells were cultured in RPMI-1640 medium (Euroclone), with 10% FBS, 1% penicillin–streptomycin solution 100X (Euroclone). Cells were incubated at 37 °C in 5% CO₂.

Benedusi M., Tamburini E., Sicurella M., Summa D., Ferrara F., Marconi P., Cervellati F., Costa S, & Valacchi G. (2022). The Lesson Learned from the COVID-19 Pandemic: Can an Active Chemical Be Effective, Safe, Harmless-for-Humans and Low-Cost at a Time? Evidence on Aerosolized Hypochlorous Acid. International Journal of Environmental Research and Public Health, 19(20), 13163.

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SILAC Labeling of HeLa and MA104 Cells

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Human epithelial adenocarcinoma HeLa cells (ATCC® CCL-2™) and African green monkey kidney epithelial cells (MA104) were propagated in Dulbecco's modified Eagle's medium (DMEM) (Gibco-BRL, Gaithersburg, MD) supplemented with heat-inactivated 10% fetal bovine serum (FBS) (Gibco/BRL) and 1% penicillin-streptomycin solution 100X (EuroClone), at 37 °C in an atmosphere of 5% of CO₂. HeLa cells were SILAC-labelled using SILAC DMEM (#89985, ThermoScientific) deficient in lysine and arginine, supplemented with 10% dialyzed fetal bovine serum (Invitrogen). To this medium, the appropriate amino acids were added as follows: unlabelled l-lysine (Lys0) and l-arginine (Arg0) (both from Sigma) were used to obtain light-labelled cells, and ¹³C₆¹⁵N₄l-arginine (Arg10, CNLM-539, Cambridge Isotope Laboratory) and ¹³C₆¹⁵N₂l-lysine (Lys8, CNLM-291, Cambridge Isotope Laboratory) were used to obtain heavy-labelled cells. The final amino acid concentration corresponded to the standard DMEM composition (i.e.84 mg/l arginine and 146 mg/l lysine). Cells were cultured for at least seven replications to achieve complete labelling. Full protein labelling was verified by geLC-MS analysis (data not shown) [11 (link)].

Civra A., Colzani M., Cagno V., Francese R., Leoni V., Aldini G., Lembo D, & Poli G. (2019). Modulation of cell proteome by 25-hydroxycholesterol and 27-hydroxycholesterol: A link between cholesterol metabolism and antiviral defense. Free Radical Biology & Medicine, 149, 30-36.

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EBV-Immortalized PBMC Cell Culture

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Epstein–Barr Virus (EBV) immortalized PBMCs were provided by Galliera Genetic Bank, from lymphocytes of the two patients, their parents, and four healthy controls, including two children (cell lines 281 and 283) and two adults (cell lines 519 and 550). The immortalized cells were maintained in RPMI 1640 medium without L-Glutamine with phenol red (Euroclone, Milan, Italy) supplemented with 10% fetal bovine serum (FBS South America origin EU Approved, Euroclone, Milan, Italy), 1% L-Glutamine 100X 200 mM (Euroclone, Milan, Italy), and 1% penicillin–streptomycin solution 100X (Euroclone, Milan, Italy) at 37 °C in a 5% CO₂ atmosphere. Each cell line was synchronized with thymidine treatment, which requires an inoculum of 500,000 cells/mL to be cultured for 24 h in a complete medium enriched with 1% thymidine 200 mM (Merck Life Science, Milan, Italy), capable of blocking cells in the G1 phase, and for the following 24 h in complete medium without thymidine, to restart growth starting from the same stage of the cell cycle, at 37 °C in a 5% CO₂ atmosphere. At the end of the treatment, the cells were washed with HEPES 50 mM (Merck Life Science, Milan, Italy), to prevent cryo-induced pH changes, and pellets of 5 × 10⁶ cells were stored at −80 °C.

Tritto V., Capitanio D., Gelfi C, & Riva P. (2023). Changes of RAS Pathway Phosphorylation in Lymphoblastoid Cell Lines from Noonan Syndrome Patients Carrying Hypomorphic Variants in Two NS Genes. International Journal of Molecular Sciences, 24(4), 4035.

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Culturing K-562 Chronic Myeloid Leukemia Cells

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The American Type Culture Collection (ATCC) in Manassas, Virginia, USA, provided the K-562 human chronic myeloid leukemia cell line, which was then frozen in liquid nitrogen. After achieving 70–80% confluency, cells were passaged twice weekly, and modest transit numbers (≤ 20) were used in all tests. To make sure cultures were free of contamination, Lonza MycoAlert™ mycoplasma testing kits were employed in accordance with the manufacturer's recommendations. In addition to 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Biowest, South America Origin), 1% penicillin–streptomycin solution 100X (EuroClone, Italy), and 1% L-glutamine 100X (EuroClone, Italy), cells were sub-cultured in RPMI-1640 medium 1X (EuroClone, Italy). In an incubator with 5% CO₂ that was humidified, cells were kept at 37 °C (NU-5810E, NUAIRE, Plymouth, MN 55447, USA).

Al-Ali L., Al-Ani R.J., Saleh M.M., Hammad A.M., Abuarqoub D.A., Abu-Irmaileh B., Naser A.Y., Najdawi M.M., Abbas M.M, & Alyoussef Alkrad J. (2023). Biological evaluation of combinations of tyrosine kinase inhibitors with Inecalcitol as novel treatments for human chronic myeloid leukemia. Saudi Pharmaceutical Journal : SPJ, 32(2), 101931.

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CFTR Inhibitor and Potentiator Assay

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RPMI 1640, fetal bovine serum (FBS), penicillin–streptomycin solution 100X, L-glutamine 100X 200 mM, Lympholyte^®-H, and prestained protein SHARPMASS VI MW marker were purchased from Euroclone SpA (Milan, Italy); anti-MMP9 antibody, horseradish peroxidase (HRP)-linked anti-rabbit secondary antibody, protease inhibitor cocktail (100X), and phosphatease inhibitor cocktail (100X) were obtained from Cell Signaling Technology (Danvers, MA, USA); dibutyryl-cAMP, isopropyl β-D-1-thiogalactopyranoside (IPTG), yeast extract, tryptone, PGex6P1, 6,7-Dihydro-7,9-dimethyl-6-(5-methyl-2-furanyl)-11-phenylpyrimido (4′,5′,3,4) pyrrolo (1,2-a) quinoxaline –8,10 (5H,9)-dione (PPQ-102), a reversible and voltage-independent CFTR inhibitor, and the potentiator Ivacaftor (VX770) were purchased from Sigma-Aldrich (Milan, Italy); GSH-sepharose™, ECL Select™ Western Blotting Detection Reagent, and Amersham ™ Protran^® Premium 0.45-μm nitrocellulose were obtained from GE Healthcare (Chicago, IL, USA); Monocytes Isolation Kit II was purchased from Miltenyi Biotec Srl (Bologna, Italy); pEYFP-C1 plasmid was obtained from Clontech Laboratories (Mountain View, CA, USA); QuickChange site-directed mutagenesis kit was from Stratagene (San Diego, CA, USA); BamHI and EcoRI restriction enzymes by Fermentas were purchased from Life Technologies Italia (Monza, Italy).

Pedrazzi M., Vercellone S., Barberis E., Capraro M., De Tullio R., Cresta F., Casciaro R., Castellani C., Patrone M., Marengo E., Lecca P., Melotti P., Sorio C., Manfredi M, & Averna M. (2021). Identification of Potential Leukocyte Biomarkers Related to Drug Recovery of CFTR: Clinical Applications in Cystic Fibrosis. International Journal of Molecular Sciences, 22(8), 3928.

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PBMC Isolation and Functional Assays

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Ficoll-Paque PLUS (GE Healthcare, Bio-Sciences AB, Uppsala, Sweden), Percoll, red blood cell lysis buffer, sterile-filtered PBS and HBSS, endotoxin-free water (Sigma, St.
Louis, USA) were used for PBMC isolation. Cells were resuspended in complete medium, comprising RPMI 1640 Medium with 25 Mm HEPES and L-Glutamine (Sigma, St. Louis, USA), supplemented with 1% of Non-essential Amino Acid Solution 100X and 1% Penicillin Streptomycin Solution 100X (Euroclone, Milano, Italy), and 10% FBS (Sigma, St. Louis, USA).
Apo-ONE® Homogeneous Caspase-3/7 Assay (Promega, Milano, Italy) and Cell Proliferation Kit I (MTT) (Roche, Mannheim, Germany) were used for apoptosis and viability assays, respectively. Chemotaxis and phagocytosis were measured by using Zymosan A from Saccharomyces cerevisiae and fluorescein-labelled Escherichia coli bioparticles K-12 strain (Invitrogen, Oregon, USA), respectively.

Ávila G., De Leonardis D., Grilli G., Lecchi C, & Ceciliani F. (2021). Anti-inflammatory activity of citrus pectin on chicken monocytes' immune response. Veterinary immunology and immunopathology, 237.

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