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2 protocols using mizoribine

1

Comprehensive Pharmacological Reagent Acquisition

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Brefeldin A, SCH772984, 4μ8C, KIRA6, and mizoribine were purchased by Selleckchem. BAY11–7082, GSK2606414, STF-083010 were purchased by Caymanchem). N-Acetyl-L-cysteine and L-Histidine were supplied by Sigma–Aldrich, thapsigargin by Enzo Life Sciences, BAPTA-AM by ThermoFisher Scientific. Bortezomib was obtained directly from the Pharmacy Department of the Leuven University Hospital, Leuven Belgium.
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2

Cell Culture Compound Screening Protocol

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The following compounds were added into the cell culture medium at final concentrations indicated in the figure legends: rapamycin (EMD Millipore #53123-88-9) in DMSO, mizoribine (Selleckchem #S1384 and Sigma #M3047) in water, mycophenolic acid (Sigma #M3536) in methanol, ribavirin (Sigma #R9644) in water, azathioprine (Sigma #A4638) in DMSO, Q-VD-OPh (Sigma #SML0063) in DMSO, staurosporine (Tocris #1285) in DMSO, CX-5461 (Selleckchem #S2684) in DMF, hydroxyurea (Sigma #H8627) in water, AZD6738 (Selleckchem #S7693) in DMSO, guanosine (Sigma #G6752) in DMSO, adenosine (Sigma #A9251) in DMSO.
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