The plasma levels of glucose and 3-hydroxybutyric acid or β-hydroxybutyric acid (BHB) were measured using commercial kits MAK263-1KT and MAK041, respectively, both of which are from Sigma-Aldrich. The plasma concentrations of corticosterone (Cat#EIACORT, Thermo Fisher, Waltham, MA, USA) and insulin (Cat#90080, Crystal Chem, Elk Grove Village, IL, USA) were determined using ELISA kits. The protocols and quantification were conducted according to the manufacturer instructions. All measurements were conducted in duplicates at the least, and the samples for which the volumes were insufficient after non-targeted metabolomic analyses were omitted from further analysis. The quantified results were analyzed by one-way ANOVA, followed by Fisher’s LSD post hoc test.
Mak263 1kt
Manufactured by Merck Group
Sourced in United States
MAK263-1KT is a laboratory equipment product manufactured by Merck Group. It is a reagent kit designed for the quantitative determination of arginine in various sample types. The kit contains the necessary components to perform the assay, such as buffers and reagents. The core function of this product is to enable the analysis and quantification of arginine levels in a sample.
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2 protocols using mak263 1kt
1
Metabolic and Hormonal Analysis Protocol
2
Insulin Resistance Assessment Protocol
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Blood samples were centrifuged (6,000×g/10 min) to get clear supernatants. Insulin and glucose concentrations were determined in plasma samples. Insulin (RAB0817-1KT, Sigma-Aldrich, Darmstadt, GER) and glucose (MAK263-1KT, Sigma-Aldrich, Darmstadt, GER) and triglycerides (Cayman Chemical, nº 10010303, Michigan, USA) were measured using commercial kits. Insulin resistance (IR) was defined as the homeostatic model assessment of insulin resistance (HOMAir) value and its behaviour was studied according to the treatment received. HOMAir was calculated according to the following formula 19 : [insulin (μU/ml)*glycaemia (mg/dL)]/405.
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