Abk pentra 400

Four milliliters of venous blood was collected into EDTA-treated Vacutainer tubes (Becton Dickinson; Franklin lakes, NJ USA). Samples for complete blood counts were analyzed using a Coulter counter at the Uganda Cancer Institute laboratory. HIV sero-status was assessed at the MNU side laboratory using Determine HIV-1/2 (Abbot Laboratories USA) rapid tests and HIV-1/2 Stat-Pak Dipstick Assay kit for children aged ≥18 mo. For those <18 mo of age, their HIV status was confirmed using an HIV DNA/polymerase chain reaction (PCR) test done at Baylor HIV clinic. Plasma was obtained by centrifuging at 1300–2200 g at ambient temperature for 10 min, and stored at −80°C prior to shipment on dry ice to the University of Copenhagen, Denmark. Plasma C-reactive protein (CRP) was analyzed by the high-sensitivity kit on an ABK Pentra 400 (Horiba; Montpellier, France). Detection of plasma citrulline has been described in detail elsewhere (40 (link)). Briefly, the LC-MS method (41 ) was used. Samples were processed with Sirocco protein precipitation plate (Waters) and separated on an ACQUITY UPLC HSS T3 Column (Waters) using an ultra-performance LC in tandem with triple quadrupole detector MS. l-Citrulline-4,4,5,5-d4 (Sigma-Aldrich) was used as an internal standard. Quantification of citrulline was carried out using QuanLynx (Waters).