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Elexsys e500 epr spectrometer

Manufactured by Oxford Instruments
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The ELEXSYS E500 EPR spectrometer is a laboratory instrument designed for electron paramagnetic resonance (EPR) spectroscopy. It is capable of detecting and analyzing unpaired electrons in a sample. The instrument operates at X-band microwave frequencies and can be used to study a wide range of materials, including solids, liquids, and gases.

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Lab products found in correlation

Cf935 cryostat, by Oxford Instruments (2 mentions) En 4118x md4, by Bruker (2 mentions) Esr900, by Oxford Instruments (2 mentions) Elexsys e580 spectrometer, by Bruker (2 mentions) Esr900 helium flow cryostat, by Oxford Instruments (1 mentions) Esr 910, by Oxford Instruments (1 mentions) Er 035 gauss meter, by Bruker (1 mentions)

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Elexsys E500 spectrometer (7 citations)

4 protocols using elexsys e500 epr spectrometer

1

Cryogenic CW-EPR and ENDOR Spectroscopy

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Continuous wave X-band (~9.4 GHz) EPR spectra were obtained using
a Bruker ELEXSYS E500 EPR spectrometer operating at cryogenic temperatures via
an Oxford Instruments ESR900 liquid helium cryostat interfaced with an ITC503
temperature controller from the same manufacturer. Samples were 250 μl in
Wilmad 4 mm o.d. quartz tubes. Sample temperatures were as stated, microwave
power was 10 μW, modulation amplitude was 1.5 G. Pulsed electron nuclear
double resonance (ENDOR) spectra were obtained at 65 K using a Bruker ELEXSYS
E580 spectrometer equipped with an EN 4118X-MD4 dielectric resonator.
Temperature control was effected using an Oxford Instruments CF935 cryostat and
ITC503 temperature controller. The Davies pulsed ENDOR sequence was employed at
intermediate Q using soft microwave pulses and FID detection (π/2 = 200
ns) with a 9 ms radiofrequency π pulse.
White M.D., Payne K.A., Fisher K., Marshall S.A., Parker D., Rattray N.J., Trivedi D.K., Goodacre R., Rigby S.E., Scrutton N.S., Hay S, & Leys D. (2015). UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis. Nature, 522(7557), 502-506.
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2

Cryogenic CW-EPR and ENDOR Spectroscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols
Continuous wave X-band (~9.4 GHz) EPR spectra were obtained using
a Bruker ELEXSYS E500 EPR spectrometer operating at cryogenic temperatures via
an Oxford Instruments ESR900 liquid helium cryostat interfaced with an ITC503
temperature controller from the same manufacturer. Samples were 250 μl in
Wilmad 4 mm o.d. quartz tubes. Sample temperatures were as stated, microwave
power was 10 μW, modulation amplitude was 1.5 G. Pulsed electron nuclear
double resonance (ENDOR) spectra were obtained at 65 K using a Bruker ELEXSYS
E580 spectrometer equipped with an EN 4118X-MD4 dielectric resonator.
Temperature control was effected using an Oxford Instruments CF935 cryostat and
ITC503 temperature controller. The Davies pulsed ENDOR sequence was employed at
intermediate Q using soft microwave pulses and FID detection (π/2 = 200
ns) with a 9 ms radiofrequency π pulse.
White M.D., Payne K.A., Fisher K., Marshall S.A., Parker D., Rattray N.J., Trivedi D.K., Goodacre R., Rigby S.E., Scrutton N.S., Hay S, & Leys D. (2015). UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis. Nature, 522(7557), 502-506.
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3

EPR Spectroscopy of Cryogenic Samples

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EPR spectra were obtained using a Bruker ELEXSYS E500 EPR spectrometer equipped with a Super High Q (ER 4118-SHQ) resonator coupled to an Oxford Instruments ESR900 helium flow cryostat for temperature control. Spectra were acquired at 20 K using 10 μW microwave power, 100 KHz modulation frequency, and 1 G modulation amplitude.
Gahloth D., Fisher K., Payne K.A., Cliff M., Levy C, & Leys D. (2022). Structural and biochemical characterization of the prenylated flavin mononucleotide-dependent indole-3-carboxylic acid decarboxylase. The Journal of Biological Chemistry, 298(4), 101771.
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4

EPR Spectroscopy of Cryo-Cooled Samples

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EPR spectra were recorded at ACERT on a Bruker ElexSys E500 EPR spectrometer at 9.4 GHz, using an ESR910 liquid-helium cryostat (Oxford Instruments) maintained at 12 K. The spectrometer settings were as follows: modulation frequency, 100 kHz; modulation amplitude, 6 Gauss; microwave power, 0.02 mW. The field sweeps were calibrated with a BRUKER ER 035 Gauss meter, and the microwave frequency was monitored with a frequency counter. Data acquisition and manipulation were performed with Xepr software.
Calvey G.D., Katz A.M., Zielinski K.A., Dzikovski B, & Pollack L. (2020). Characterizing Enzyme Reactions in Microcrystals for Effective Mix-and-Inject Experiments using X-ray Free-Electron Lasers. Analytical chemistry, 92(20), 13864-13870.
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