Uveitis was induced by intracameral injection of 100 ng of E. coli LPS (Sigma #L4391, St. Louis, MO) in 20 μl PBS (Mediatech, Manassas, VA) into the right eye using a 29 gauge needle, while the rabbits were anesthetized with ketamine HCl (25 mg/kg, Ft. Dodge) and xylazine (2 mg/kg, Lloyd) IM. Topical proparacaine (0.5%, Akorn, Lake Forest, IL) was applied to the ocular surface prior to intraocular injections. The ocular surface was prepared for the injection procedure using a dilute solution of 5% Povidone iodine (Aurora Pharmaceutical, Northfield, MN) in 0.9% saline (Phoenix, Burlingame, CA). During anesthesia (preparation and recovery) the cornea was protected from drying by irrigation with Balanced Salt Solution (Akorn) or, following intraocular injection, the application of ocular lubricant (Refresh Tears, Allergan) or Bacitracin-Polymixin B Preservative Free Ophthalmic Ointment (Akorn). The left eye received all the pre- and post- injection treatments, but not the LPS or PBS injection and thus was used as a procedural control for ocular preparation.
Balanced salt solution
Manufactured by Akorn
Balanced Salt Solution is a laboratory product designed to maintain a specific ionic balance. It serves as a basic medium for various cell culture and biological applications. The solution contains a carefully formulated combination of salts, such as sodium, potassium, calcium, and magnesium, to support the physiological environment required for cell growth and maintenance.
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2 protocols using balanced salt solution
1
Induction of Uveitis in Rabbits
2
Induction of Uveitis in Rabbits
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Uveitis was induced by intracameral injection of 100 ng of E. coli LPS (Sigma #L4391, St. Louis, MO) in 20 μl PBS (Mediatech, Manassas, VA) into the right eye using a 29 gauge needle, while the rabbits were anesthetized with ketamine HCl (25 mg/kg, Ft. Dodge) and xylazine (2 mg/kg, Lloyd) IM. Topical proparacaine (0.5%, Akorn, Lake Forest, IL) was applied to the ocular surface prior to intraocular injections. The ocular surface was prepared for the injection procedure using a dilute solution of 5% Povidone iodine (Aurora Pharmaceutical, Northfield, MN) in 0.9% saline (Phoenix, Burlingame, CA). During anesthesia (preparation and recovery) the cornea was protected from drying by irrigation with Balanced Salt Solution (Akorn) or, following intraocular injection, the application of ocular lubricant (Refresh Tears, Allergan) or Bacitracin-Polymixin B Preservative Free Ophthalmic Ointment (Akorn). The left eye received all the pre- and post- injection treatments, but not the LPS or PBS injection and thus was used as a procedural control for ocular preparation.
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