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Iodocetamide

Manufactured by Merck Group
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Iodocetamide is a lab equipment product used as a reagent. It is a chemical compound that serves as an alkylating agent, primarily used in biochemical and molecular biology applications.

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Lab products found in correlation

Dithiothreitol (dtt), by Merck Group (3 mentions) Rgd peptide, by Merck Group (1 mentions) Q vd oph, by MP Biomedicals (1 mentions) Cilengitide, by Merck Group (1 mentions) Fak inhibitor 14 fak 14, by Bio-Techne (1 mentions) Y 27632, by Merck Group (1 mentions) H1152, by Merck Group (1 mentions) Sodium deoxycholate, by Merck Group (1 mentions) Pierce protein assay kit, by Thermo Fisher Scientific (1 mentions) Triethylammonium bicarbonate, by Thermo Fisher Scientific (1 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) Urea, by Merck Group (1 mentions) Ammonium bicarbonate, by Merck Group (1 mentions) Sequencing grade modified trypsin, by Promega (1 mentions) Lc ms grade water, by Merck Group (1 mentions) Sequencing grade trypsin, by Promega (1 mentions) Ltq orbitrap velos mass spectrometer, by Thermo Fisher Scientific (1 mentions) Trifluoroacetic acid, by Merck Group (1 mentions)

4 protocols using iodocetamide

1

Inhibition of Rho Kinase and FAK in Cell Signaling

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The ROCK inhibitors Y-27632 (Rho Kinase inhibitor VI) and H-1152 were obtained from EMD Millipore (Billerica, MA, USA); Q-VD-OPh from MP Biomedicals (Eschwege, Germany); and GT from AppliChem (Darmstadt, Germany). The CNFy toxin (150 ng/ml) from Yersinia pseudotuberculosis and the C2I/C3 fusion toxin (Clostridium botulinum and Clostridium Limosum, respectively) combined with the C2II toxin from Clostridium botulinum (collectively termed C3 toxin) was produced and purified as described33 (link),34 (link). C3 toxin is a binary toxin consisting of C2II (200 ng/ml) and C2I/C3 (100 ng/ml) mixed in cell culture media. The FAK inhibitor 14 (FAK14) was purchased from Tocris (Bristol, UK), and Cilengitide, a cyclic pentapeptide RGD compound (cyclo-[RGDfN(Me)V])46 (link), the RGD peptide, DTT and iodocetamide were from Sigma (Taufkirchen, Germany).
Haun F., Neumann S., Peintner L., Wieland K., Habicht J., Schwan C., Østevold K., Koczorowska M.M., Biniossek M., Kist M., Busch H., Boerries M., Davis R.J., Maurer U., Schilling O., Aktories K, & Borner C. (2018). Identification of a novel anoikis signalling pathway using the fungal virulence factor gliotoxin. Nature Communications, 9, 3524.
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2

Mass Spectrometry Sample Preparation

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Sodium deoxycholate (SDC), urea, sodium dodecyl sulfate (SDS), dithiothreitol (DTT), iodocetamide (IAA), ammonium bicarbonate (ABC), methanol, and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Triethylammonium bicarbonate (TEAB) and the Pierce Protein Assay Kit were purchased from Thermo-Fisher (Waltham, MA, USA). Sequencing grade modified trypsin was purchased from Promega (Madison, WI, USA). LC-MS grade water and LC-MS grade acetonitrile (ACN) were purchased from Merck (Darmstadt, Germany).
Krösser D., Dreyer B., Siebels B., Voß H., Krisp C, & Schlüter H. (2021). Investigation of the Proteomes of the Truffles Tuber albidum pico, T. aestivum, T. indicum, T. magnatum, and T. melanosporum. International Journal of Molecular Sciences, 22(23), 12999.
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3

N-Glycan Release and Peptide Analysis

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After the in-gel release of N-glycans by PNGase F treatment proteins were digested with trypsin according to the method outlined by Shevchenko et al. [57] . The procedure involved reducing the proteins with 10 mM DTT (Sigma-Aldrich), followed by carbamidomethylation with 100 mM iodocetamide (Sigma-Aldrich), and subsequent digestion with sequencing-grade trypsin (Promega). To extract the resulting peptides, acetonitrile was used, and the samples were then dried in a vacuum centrifuge before being dissolved in a solution containing 2% (v/v) acetonitrile and 0.1% (v/v) trifluoroacetic acid (Sigma-Aldrich) for subsequent LC-MS/MS analysis. The analysis was performed using a LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled online to a nano-flow ultra-high-pressure liquid chromatography system (RSLC, Thermo Fisher Scientific).
Reverse-phase chromatography and mass spectrometry was carried out as described previously [58] . For data analysis, the MaxQuant proteomics software suite version 1.2.2.5 [59] was utilized, and peak lists were searched against the SIVmac239/316 Env sequence using the Andromeda search engine version 1.1.0.36 [60] .
Karsten C.B., Buettner F.F.R., Cajic S., Nehlmeier I., Roshani B., Klippert A., Sauermann U., Stolte-Leeb N., Reichl U., Gerardy-Schahn R., Rapp E., Stahl-Hennig C, & Pöhlmann S. (2024). Macrophage- and CD4+ T cell-derived SIV differ in glycosylation, infectivity and neutralization sensitivity. PLoS pathogens, 20(5).
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4

Molecular Biology Reagents Protocol

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Molecular biology-grade reagents were purchased commercially. Poly-L lysine, protease inhibitor cocktail, H2DCFDA, DAPI, Flouramaount, SMCC (Succinimidyl- trans-4-(N-maleimidylmethyl)cyclohexane-1-carboxylate), HIV-TAT1, Iodocetamide, DMF, Cyclodextrin, Cycloheximide (CHX), Etoposide, Methyl β-cyclodextrin (MβCD), anti-His (SAB4301134), trypan blue, and a BCA protein estimation kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ni+2-NTA beads, LipofectamineLTX plus transfection reagent Prestoblue viability assay kit, IL-1 beta Human ELISA Kit (BMS224HS), and Alexa fluors were purchased from Invitrogen (Life Technologies, Carlsbad, California, USA). A dual glow luciferase assay kit was purchased from Promega (Madison, WI, USA). The death receptor ligands CD 95L and TNF-α were obtained from ProSpec (Rehovot, Israel). HA14-1 was obtained from Maybridge (Cornwall, UK). The details of the antibodies used in this study are provided in Supplementary Material Table S1. All other chemicals used were of analytical grade and purchased from Merck (Darmstadt, Germany).
Ranjan K., Waghela B.N., Vaidya F.U, & Pathak C. (2020). Cell-Penetrable Peptide-Conjugated FADD Induces Apoptosis and Regulates Inflammatory Signaling in Cancer Cells. International Journal of Molecular Sciences, 21(18), 6890.
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