Portable incubator

Manufactured by Minitube

Sourced in Germany, United States

The Portable Incubator is a compact, self-contained unit designed for maintaining a controlled environment. It provides stable temperature and humidity settings to support the growth and development of various samples or specimens.

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Lab products found in correlation

Gentamycin sulfate, by Thermo Fisher Scientific (2 mentions) Gentamicin reagent solution, by Thermo Fisher Scientific (2 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Gentamycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)

6 protocols using portable incubator

Cloned Embryo Transfer in Pigs

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After culturing under similar conditions for 4–6 h (at 14–16 h post-activation), siRNA1-injected and non-injected cloned embryos at the 1–2 cell stage were
loaded into a transparent transfer tube and kept in a portable incubator (Minitube, WI, USA.) during transportation to the farm where the recipient sows were
housed. Landrace sows in parity 2–5, from a same farm, with a similar genetic background, and which showed a natural standing estrus within 40–42 h prior to
embryo transfer were used as embryo recipients. The sows were anesthetized with an anesthetic (Quanmianbao) consisting of ketamine (25 mg/kg body weight) and
xylazine (1.1 mg/kg body weight) for induction and 3% isoflurane for maintenance. The ovaries and oviducts were exposed by making ~7-cm incision along the
midline of the sow’s abdomen between the last two pairs of teats. Follicles in the ovaries were examined to determine the recipient’s ovulation status by
criteria similar to that previously reported [14 (link)]. The cloned embryos with 0.1 ml culture medium were delivered directly
into the recipient’s oviduct (at 2/3 length of the oviduct) using a 1-ml syringe attached to a transparent transfer tube. The transfer tube was examined
subsequently under a microscope to ensure the transfer of all the embryos.

ZENG F., HUANG Z., YUAN Y., SHI J., CAI G., LIU D., WU Z, & LI Z. (2016). Effects of RNAi-mediated knockdown of Xist on the developmental efficiency of cloned male porcine embryos. The Journal of Reproduction and Development, 62(6), 591-597.

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Cloned Porcine Embryo Transfer Protocol

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Embryos at the 2-cell stage were selected and loaded into transfer tubes 22–26 h after activation. The selected embryos were transported to a pig farm with a portable incubator (Minitube, Delavan, WI, USA). Within 40–46 h prior to embryo transfer, estrous-synchronized Yorkshire sows were used as recipients. Anesthesia was induced with ketamine (25 mg/kg body weight) and xylazine (1.1 mg/kg body weight), respectively, and maintained with 3% isoflurane. The ovaries and oviducts of sows were surgically exposed. A syringe was used to deliver the cloned embryos to the oviducts of sows. Ultrasound examination was performed to monitor the pregnancy status of the recipient sows at 1 mo after the embryo transfer. The recipients gave birth to cloned piglets through natural birth. If natural farrowing did not occur until gestation day 116, the recipient sows were injected with a prostaglandin analog (cloprostenol; 200 g/recipient) to induce farrowing, and the farrowing conditions of the sows were recorded.

Dong Y., Wu X., Peng X., Yang L., Tan B., Zhao H., Zheng E., Hong L., Cai G., Wu Z, & Li Z. (2022). Knockdown of YY1 Inhibits XIST Expression and Enhances Cloned Pig Embryo Development. International Journal of Molecular Sciences, 23(23), 14572.

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Oocyte Maturation Protocol for In Vitro Fertilization

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Cumulus-oocyte complex quality was classified based on the number of layers of compact cumulus cells and the presence of homogenous cytoplasm from I to IV (I-highest to IV-poorest) [16] (link). Once classified, COCs were washed and transferred to a 35-mm Petri dish containing 3 ml of maturation medium, consisting of TCM 199 with Earl's salts and 25 mM HEPES, 10% v/v FBS, 50 mM cysteamine, 5 µg/ml FSH (NIH-FSH-P1; Folltropin-V; Bioniche Animal Health, Belleville, Ontario, Canada) and 0.1% v/v gentamycin sulfate (Gibco, Thermo Fisher Scientific, gentamicin reagent solution, 50 mg/ml). They were then transferred to 1.8 ml Eppendorf tubes filled with pre-equilibrated maturation medium and transported to the IVF laboratory (8 h from the farm) in a portable incubator (Minitube, Germany) set at 37 °C.

Berdugo J., Tarazona A., Echevererry J.J., Konrad J.L., Crudeli G., & Herrera A.L. (2020). Differences in Parameters of an Embryo In Vitro Production Program between Cattle (Bos Indicus) and Buffaloes (Bubalus bubalis). Journal of Buffalo Science, 9, 29-37.

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Oocyte Maturation Protocol for In Vitro Fertilization

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COVID-19 Semen Sampling Protocol

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All the participants were clearly instructed on collecting their semen samples into a sterile container by masturbation. Following 3-4 days of sexual abstinence, participants in the CON group reported to the lab on the respective sampling days to provide semen samples at the site. In the COVID-19 group, the patients who could not attend the laboratory due to their health condition and whose distance from the laboratory was close (n = 9; 10.7%), samples were collected at home and delivered to the laboratory through a portable incubator (Minitüb GmbH, Tiefenbach -Germany) within 30 min of collection by a researcher wearing suitable personal protective equipment. The remaining patients (n = 75; 89.3%) were provided semen samples on-site. The duration of sexual abstinence before taking the semen sample was not standardized for the COVID-19 group. Semen samples were taken at baseline (24 h after hospital discharge) and follow-ups at 10, 20, 30, 40, 50, and 60 days after baseline. Using an identical protocol, SARS-CoV-2 detection in semen samples was performed (Huang et al. 2020) (link). The time between the confirmatory diagnosis of COVID-19 and first semen collection was 13.2 ± 4.9 days.

Hajizadeh Maleki B, & Tartibian B. (2021). COVID-19 and male reproductive function: a prospective, longitudinal cohort study. Reproduction (Cambridge, England), 161(3).

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In vitro maturation of buffalo oocytes

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Unless otherwise stated, all chemicals were purchased from Sigma Argentina. Recovered cumulus-oocyte complexes (COCs) were washed and maintained in TCM 199 supplemented with Hank's salts, 10% v/v FBS (Gibco, Thermo Fisher Scientific, Argentina) and 0.1% v/v gentamicin (Gibco, Thermo Fisher Scientific, gentamicin reagent solution, 50 mg/ml) for classification according to quality. Cumulus-oocyte complex quality was classified according to IETS guidelines (quality I-highest to quality IV-poorest) and previous reports for buffalo (Di Francesco et al., 2011) based on the number of layers of compact cumulus investments and presence of homogenous cytoplasm. Once classified, the COCs were washed and transferred to a 35-mm Petri dish containing 3 ml of maturation medium consisting of TCM 199 with Earl's salts and 25 mM Hepes, 10% v/v FBS, 50 mM cysteamine, 5 μg/ml FSH (NIH-FSH-P1; Folltropin-V; Bioniche Animal Health, Belleville, Ontario, Canada) and 0.1% v/v gentamycin sulfate (Gibco, Thermo Fisher Scientific, gentamicin reagent solution, 50 mg/ml). They were then transferred to 1.8 ml Eppendorf tubes filled to the top with pre-equilibrated maturation medium and allowed to mature in transit to the IVF laboratory (8 h from farm) in a portable incubator (Minitube, Germany) set at 37 °C.

Konrad J., Clérico G., Garrido M.J., Taminelli G., Yuponi M., Yuponi R., Crudeli G, & Sansinena M. (2017). Ovum pick-up interval in buffalo (Bubalus bubalis) managed under wetland conditions in Argentina: Effect on follicular population, oocyte recovery, and in vitro embryo development. Animal reproduction science, 183.

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