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8 protocols using phosphoric acid

1

Fabrication of Doped Silicon Wafer

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Phosphate buffer solution (PBS) was made using phosphoric acid (Daejung, Korea), potassium phosphate monobasic and potassium phosphate dibasic (99.9%) from Sigma-Aldrich. Highly doped n-type silicon wafer (arsenic-doped, < 100 > -oriented) with a resistivity as low as 0.005 Ω cm was obtained from STC (Japan). AZ4620 photoresist was purchased from Merck (USA).
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2

Emulsion Preparation with Volatile Organic Compounds

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Emulsions were prepared using canola oil purchased from a local supermarket. Davisco Foods International, Inc. (Eden Prairie, Minnesota, USA) provided β-lactoglobulin with a purity above 95%. Sodium azide and phosphoric acid were obtained from Daejung Chemicals & Metals Co., Ltd. (Siheung, Gyeonggi, Republic of Korea). Four volatile organic compounds (VOCs) of different physicochemical properties (Table 1) were used: ethyl acetate, 1-penten-3-one, trans-2-hexenal and nonanal (Sigma-Aldrich, St. Louis, Missouri, USA). The purity of all VOCs was above 95%.

Physicochemical properties of selected volatile organic compounds (VOCs)

Volatile flavour compoundChemical formulaBoiling point (°C)Log PaVapour pressure (mmHg) 25 °CWater solubility (g/L) 25 °C
ethyl acetateC4H8O2770.77388
1-penten-3-oneC5H8O1031.03120
trans-2-hexenalC6H10O1461.84.65.3
nonanalC9H18O1913.50.50.1

Lide (2005 ) and TGSC (2009 )

aPartition coefficient between octanol and water

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3

Graphite-based Material Synthesis

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Graphite (Sigma-Aldrich, 325 mesh), sulfuric acid (H2SO4, 95%, Daejung), phosphoric acid (H3PO4, 85%, Daejung), potassium permanganate (KMnO4, 99.0%, Daejung), hydrogen peroxide (H2O2, 30%, Daejung), hydrochloric acid (HCl, 35%, Daejung), and potassium hydroxide (KOH, flake, Daejung), were used for the synthesis process. These chemicals were used as received without further purification. Deionized water was used in all experiments.
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4

Fabrication of Nanoporous Alumina Ceramic Supporter

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An aluminium anodization process was performed to fabricate a ceramic supporter with a nanoporous structure. Nanoporous alumina is formed when a voltage is applied after immersing aluminium in an electrolyte solution with an anode and platinum as a cathode. In this study, high purity (99.999%) aluminium was anodized from 0.3 M phosphoric acid (Daejung chem) electrolyte, which can form a large pore diameter of 200~400 nm. The thickness of the ceramic supporter was controlled by deriving the sum of the charges applied through current monitoring during the aluminium anodization process. After the aluminium anodizing process, the unnecessary aluminium was removed using hydrochloric acid etchant. A barrier layer of nanoporous alumina was removed with a 0.1 M phosphoric acid etchant to form through-hole nanoporous alumina.
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5

Synthesis of Graphene-CMC Composite

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Sodium carboxymethyl cellulose (CMC, average M  ~ 250,000) and graphite flakes were purchased from Sigma Aldrich Korea. Sulfuric acid (H2SO4; 98%) and phosphoric acid (H3PO4; 85%) were purchased from DAEJUNG and DUKSAN, respectively. Potassium permanganate (KMnO4; 99.3%) and hydrogen peroxide (H2O2; 30%) were purchased from OCI Company Ltd., Korea. Hydrochloric acid (HCl; 35%–37%) and anhydrous ethyl alcohol (99.5%) were purchased from SAMCHUN and DAEJUNG, respectively. CNTs were supplied by Nanosolution Co., Korea.
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6

Fabrication of Hydrophobic Demolding Reagents

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Aluminum foil (99.99%) was obtained from Alfa Aesar (Karlsruhe, Germany). Perchloric acid (70%), ethyl alcohol anhydrous (99.9%), oxalic acid (99.5%), chromium oxide (Ⅳ) (99%), phosphoric acid (99.5%), and acetone (99.5%) were purchased from Daejung Chemicals (Siheung, Korea). The fluorine hydrophobic demolding reagent, UV-curing polyurethane acrylate resins, and additives were synthesized by referring to the recent report [30 (link)].
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7

HPLC Analysis of A7OG and Baicalin

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The A7OG standard and baicalin were purchased from Biopurify (Chengdu, China) and Alladin-e (Shanghai, China), respectively. The acetonitrile, methanol, and water were HPLC-grade. Extra-pure grade dichloromethane (J.T.Baker) and phosphoric acid (Daejung, Korea) were used in this experiment.
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8

Synthesis and Characterization of Graphene Oxide

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The chemicals used for the present research were all analytical grade and used as obtained. Graphite powder (<20 [46 (link)] synthetic), hydrogen peroxide (H2O2: 30% w/w in H2O), potassium permanganate (KMnO4: ACS reagent grade), ammonia solution (NH4OH: 25% for analysis EMSURE ISO, Reag. Ph Eur.), sulphuric acid (H2SO4: Assay 95%), thermoplastic polyurethane, nickel nitrate hexahydrate (Ni(NO3)2.6H2O: trace metal basis, 99.99%), and sodium nitrate (ACS grade, ≥99.0%) were purchased from SIGMA ALDRICH. Whereas sodium hydroxide (NaOH, 98%), dimethylformamide (Assay 99.9%, SGR grade), ethylene glycol (Assay 99%, FP 116 °C), phosphoric acid (H3PO4: 85% HPLC grade), and hydrochloric acid (HCl: assay 36%) were obtained from DAEJUNG. Deionized water was used for the preparation of all solutions.
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