Gc 7890b ms 240

The samples were analyzed using the Agilent GC 7890B–MS 240 (Agilent, CA, United States) ion trap Gas Chromatography technology equipped with MS detector (GC-MS). Injections were in a splitless mode for 0.75 min, using a 2 mm I.D. non-deactivated direct liner. The separation was carried out on an HP-5MS capillary column (30 m × 0.250 mm; 0.25-μm film thickness). The analysis was carried out in full scan mode for 60 min. Autosampler injected 1 µL of each sample and the separation was performed using the column in split mode and with the ionization range from 50 to 1000 mV. The carrier gas was helium with a flow rate of 1.1 mL/min. The injector temperature was set at 280 °C and GC oven temperature was programmed at 40 °C for 2 min, then a slope from 50 °C up to 250 °C maintained for 20 min. The analysis was carried out in full scan mode for 60 min.

The samples were solubilized in methanol and analyzed using an Agilent GC 7890BMS 240 ion trap gas chromatography (GC) system, equipped with MS detector and HP-5MS capillary column (30 m × 250 µm, film thickness 0.25 µm). Injector temperature was set at 280 °C and GC oven temperature was started at 40 °C for 2 min, then increased by 5 °C/min to 250 °C and held 20 min at this temperature. Analysis was carried in full scan mode for 60 min. The gas carrier helium was used at a flow rate of 1 mL/min. Samples of 1 µL were injected with the split mode and an ionization range from 50 to 1000 mV. Identification of metabolites was done by comparing their mass spectra with those referenced in the Library (NIST).

The samples were analyzed using the Agilent GC 7890B MS 240 ion trap gas chromatography technology equipped with an MS detector (GC-MS). Injections were in a splitless mode for 0.75 min, using a 2 mm I.D. non-deactivated direct liner. The separation was carried out on an HP-5MS capillary column (30 m × 0.250 mm; 0.25 μm film thickness). The analysis was carried out in full scan mode for 60 min. The autosampler injected 1 µL of each sample and the separation was performed using the column in split mode and an ionization range from 50 to 1000 mV. The carrier gas was helium with a flow rate of 1.1 mL/min. The injector temperature was set at 280 °C and the GC oven temperature was programmed at 40 °C for 2 min, and then a slope at 50 °C up to 250 °C was maintained for 20 min. The analysis was carried out in full scan mode for 60 min.