At 406

Manufactured by Active Motif

AT-406 is a fluorescence-based assay kit designed to measure the activity of apoptosis-inducing proteins. It provides a quantitative readout of caspase-3/7 activity, which is a key indicator of apoptosis or programmed cell death. The kit includes all necessary reagents and can be used with a variety of cell types and sample types.

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Lab products found in correlation

Gdc 0917, by Active Motif (2 mentions) Azd 5582, by Active Motif (2 mentions) High molecular weight poly 1 c, by InvivoGen (2 mentions) Lcl161, by Novartis (2 mentions) Ifn β, by PBL Assay Science (1 mentions) Tnf α, by Enzo Life Sciences (1 mentions) On targetplus smartpool, by Horizon Discovery (1 mentions) Vp 16, by Merck Group (1 mentions) On targetplus smartpool, by Thermo Fisher Scientific (1 mentions) Tumor necrosis factor (tnf), by Enzo Life Sciences (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Staurosporine, by Merck Group (1 mentions)

2 protocols using at 406

Apoptosis Induction Pathway Analysis

Cited 1 time

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Novartis provided LCL161 (ref. 44 (link)). Tetralogic Pharmaceuticals provided Birinapant48 (link). AT-406, GDC-0917 and AZD-5582 were purchased from Active Biochem. TNF-α was purchased from Enzo. IFN-β was obtained from PBL Assay Science. Broad host range IFN-αB/D was produced in yeast and purified by affinity immunochromatography24 (link). Nontargeting siRNA or siRNA targeting cFLIP were obtained from Dharmacon (ON-TARGETplus SMARTpool). High molecular weight poly(I:C) was obtained from Invivogen.

Beug S.T., Beauregard C.E., Healy C., Sanda T., St-Jean M., Chabot J., Walker D.E., Mohan A., Earl N., Lun X., Senger D.L., Robbins S.M., Staeheli P., Forsyth P.A., Alain T., LaCasse E.C, & Korneluk R.G. (2017). Smac mimetics synergize with immune checkpoint inhibitors to promote tumour immunity against glioblastoma. Nature Communications, 8, 14278.

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Apoptosis Induction Reagent Procurement

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Novartis provided LCL161 (18) . SM-164 was provided by S. Wang (University of Michigan, USA) (54) . Birinapant was obtained from Tetralogic Pharmaceuticals (55) . OICR720 was synthesized by the Ontario Institute for Cancer Research (OICR) (56) . AT-406, GDC-0917, and AZD-5582 were purchased from Active Biochem. TNF- was purchased from Enzo. IFN- was obtained from PBL Assay Science. All siRNAs were obtained from Dharmacon (ON-TARGETplus SMARTpool) or Ambion (Silencer Select for IFR1). High-molecular weight poly(I:C) was obtained from InvivoGen. VP16, staurosporine, and cycloheximide were obtained from Sigma.

Beug S.T., Cheung H.H., Sanda T., St-Jean M., Beauregard C.E., Mamady H., Baird S.D., LaCasse E.C, & Korneluk R.G. (2019). The transcription factor SP3 drives TNF-α expression in response to Smac mimetics. Science signaling, 12(566).

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