Anti cd144

Manufactured by Thermo Fisher Scientific

Anti-CD144 is a laboratory reagent used for the detection and identification of CD144 (also known as VE-cadherin) in biological samples. CD144 is a cell-cell adhesion molecule that plays a key role in the regulation of vascular endothelial cell function. This reagent can be used in various research and analytical applications, such as flow cytometry and immunohistochemistry, to study the expression and distribution of CD144 in different cell types and tissues.

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Lab products found in correlation

Fv1000mpe confocal microscope, by Olympus (2 mentions) Anti cd31, by Santa Cruz Biotechnology (2 mentions) Anti nrp 1, by Santa Cruz Biotechnology (2 mentions) Fv10 asw 3.0 viewer software, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole dapi, by Agilent Technologies (1 mentions) Uplansapo 60xw, by Olympus (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

2 protocols using anti cd144

Immunofluorescence Staining of Endothelial Cells

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After been fixed with 4% (w/v) paraformaldehyde for 30 min, cells were permeabilized with 0.1% (v/v) Triton X-100 in PBS for 5 min. A total of 10% (v/v) goat serum was added, and the cells were incubated for 30 min to block unspecific antibody binding. Next, the cells were incubated with the following primary antibodies overnight at 4°C: anti-CD31 (Santa Cruz Biotechnology), anti-CD144 (eBioscience), anti–NRP-1 (Santa Cruz Biotechnology), and anti–α-SMA, (Chemicon). Then, the cells were washed with PBS three times and incubated with Alexa Fluor 488– or Alexa Fluor 565–conjugated secondary antibodies (Molecular Probe). The stained cultures were counterstaining with 4′,6-diamidino-2-phenylindole (DAPI) (DAKO). Confocal images were acquired with an Olympus FV1000MPE confocal microscope using an objective lens (UPlanSApo 60XW, NA 1.20, Olympus). Z-stack images were taken with 10-μm intervals. Images were analyzed using FV10-ASW 3.0 Viewer software (Olympus) and processed in ImageJ software.

Gil C.H., Chakraborty D., Vieira C.P., Prasain N., Calzi S.L., Fortmann S.D., Hu P., Banno K., Jamal M., Huang C., Sielski M.S., Lin Y., Huang X., Dupont M.D., Floyd J.L., Prasad R., Longhini A.L., McGill T.J., Chung H.M., Murphy M.P., Kotton D.N., Boulton M.E., Yoder M.C, & Grant M.B. (2022). Specific mesoderm subset derived from human pluripotent stem cells ameliorates microvascular pathology in type 2 diabetic mice. Science Advances, 8(9), eabm5559.

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Immunofluorescent Characterization of ECFCs

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ECFCs were fixed with 4% (w/v) paraformaldehyde for 30 min and permeabilized with 0.1% (v/v) Triton X-100 in PBS for 5 min. After blocking with 10% (v/v) goat serum for 30 min, cells were incubated with primary following antibodies; anti-CD31 (Santa Cruz), anti-CD144 (Ebioscience), anti-NRP-1 (Santa Cruz) and anti-a-SMA, (Chemicon) overnight at 4 °C. Cells were washed with PBS, then incubated with secondary antibodies conjugated with Alexa-488 or Alexa-565 (Molecular Probe) and visualized by confocal microscopy after counterstaining with 2 g/ml DAPI (Sigma-Aldrich). The confocal images were obtained with an Olympus FV1000 mpE confocal microscope using as an Olympus uplanSApo 60xW/1.2NA/eus objective. All the images were taken as Z-stacks with individual 10-μm thick sections at room temperature and images were analyzed using FV10-ASW 3.0 Viewer.

Prasain N., Lee M.R., Vemula S., Meador J.L., Yoshimoto M., Ferkowicz M.J., Fett A., Gupta M., Rapp B.M., Saadatzadeh M.R., Ginsberg M., Elemento O., Lee Y., Voytik-Harbin S.L., Chung H.M., Hong K.S., Reid E., O'Neill C.L., Medina R.J., Stitt A.W., Murphy M.P., Rafii S., Broxmeyer H.E, & Yoder M.C. (2014). Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony–forming cells. Nature biotechnology, 32(11), 1151-1157.

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