Gelcompar 2 version 5

A total of 24 isolates were selected based on the Rep-PCR clustering for sequencing of the 16S rRNA gene. Partial 16S rRNA gene amplification using forward primer fD1 (5´-AGA GTT TGATCC TGG CTC AG-3´) and reverse primer rD1 (5´-AAG GAG GTG ATC CAG CCG CA-3´) [40] . The PCR was carried with initial denaturation at 95 °C for 5 min; 35 cycles of 95 °C for 2 min; 42 °C for 30 s and 72 °C for 4 min; and a final elongation step at 72 °C for 10 min. The PCR products were confirmed by electrophoresis and visualized under UV light with a Gel Doc system (Bio-Rad, Hercules, CA, USA) Grouping of the rep-PCR fingerprints was performed using GELCOMPAR II version 5.10 software (Applied Maths, Sint-Martens-Latem, Belgium) based on the Dice similarity coefficient and the UPGMA algorithm to obtain a dendogram.