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Manganese dichloride

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Manganese dichloride is an inorganic compound with the chemical formula MnCl₂. It is a crystalline solid that is soluble in water and organic solvents. Manganese dichloride is commonly used as a laboratory reagent and an intermediate in the production of other manganese compounds.

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Lab products found in correlation

Methyl α d mannopyranoside, by Merck Group (3 mentions) C18 omix tip, by Agilent Technologies (3 mentions) D lactose, by Merck Group (3 mentions) Sequencing grade trypsin, by Promega (2 mentions) Acetyl d18 glucosamine, by Merck Group (2 mentions) Panc 1 pancreatic ductal adenocarcinoma cells, by American Type Culture Collection (2 mentions) Concanavalin a (cona), by Merck Group (2 mentions) Copper sulfate, by Merck Group (1 mentions) Zinc sulfate heptahydrate, by Merck Group (1 mentions) Iron sulfate heptahydrate, by Merck Group (1 mentions) Bathocuproine, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Calcium chloride cacl2, by Thermo Fisher Scientific (1 mentions) Ammonium bicarbonate abc, by Thermo Fisher Scientific (1 mentions) N methylmorpholine, by Merck Group (1 mentions) Optima lc ms grade solvents, by Thermo Fisher Scientific (1 mentions) Amicon ultra 0.5 ml centrifugal filter, by Merck Group (1 mentions) Trifluoroacetic acid tfa, by Merck Group (1 mentions) Acs grade, by Thermo Fisher Scientific (1 mentions) Microcon filters ym 30, by Merck Group (1 mentions)

5 protocols using manganese dichloride

1

Preparation of Metal Sulfate Stock Solutions

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Copper sulfate, iron sulfate heptahydrate (FeSO4), zinc sulfate heptahydrate (ZnSO4), and manganese dichloride (MnCl2) were purchased from Sigma-Aldrich, dissolved in water as 100 mM stock solutions and stored at 4°C. The FeSO4 solution was always prepared fresh prior to use. bathocuproinedisulfonic acid was purchased from Fisher Scientific and stored as a 100 mM stock solution in water at −80°C. Stocks of test compounds were prepared in DMSO and stored at −80°C as follows: Neo (Sigma-Aldrich) 10 mM, DSF (Sigma-Aldrich) 40 mM, bathocuproine (Sigma-Aldrich) 1 mM, and GTSM 10 mM. GTSM was a kind gift from Dr. Stefan Bossmann, who synthesized the compound in his laboratory at Kansas State University following published protocols (Haeili et al., 2014 (link)).
Totten A.H., Crawford C.L., Dalecki A.G., Xiao L., Wolschendorf F, & Atkinson T.P. (2019). Differential Susceptibility of Mycoplasma and Ureaplasma Species to Compound-Enhanced Copper Toxicity. Frontiers in Microbiology, 10, 1720.
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2

Glycoproteomic analysis of PANC-1 cells

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Dithiothreitol (DTT), PNGase F and sequencing grade trypsin were from Promega (Madison, WI). Optimal LC/MS grade acetonitrile (ACN), methanol (MeOH) and water were from Fisher Scientific (Pittsburgh, PA). Concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinuscommunis agglutinin (RCA120), iodoacetamide (IAA), acetyl-D18 glucosamine, D-lactose, methyl α-D-mannopyranoside and manganese dichloride were obtained from Sigma-Aldrich (St. Louis, MO). Tris base, urea (UA), sodium chloride, ammonium bicarbonate (ABC) and calcium chloride (CaCl2) were obtained from Fisher Scientific (Pittsburgh, PA). Trifluoroacetic acid (TFA), triethylammonium bicarbonate (TEAB), N, N-dimethylformamide (DMF), 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium tetrafluoroborate (DMTMM), N-methylmorpholine (NMM) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO). Hydroxylamine solution was purchased from Alfa Aesar (Ward Hill, MA). C18 OMIX tips were obtained from Agilent (Santa Clara, CA). Hydrophilic interaction chromatography material (PolyHYDROXYETHYL A) was obtained from PolyLC (Columbia, MD). Microcon filters YM-30 (30 kDa) was purchased from Merck Millipore (Billerica, MA). PANC-1 pancreatic ductal adenocarcinoma cells were from ATCC (Manassas, VA). Duplex DiLeu tags were custom synthesized in our own lab.45 (link)
Chen Z., Yu Q., Hao L., Liu F., Johnson J., Tian Z., Kao W.J., Xu W, & Li L. (2018). Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD). The Analyst, 143(11), 2508-2519.
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3

Glycoproteomic Analysis of PANC-1 Cells

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Dithiothreitol (DTT), sequencing grade trypsin were purchased from Promega. Concanavalin A (ConA), wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA120), iodoacetamide (IAA), acetyl-D18 glucosamine, D-lactose, methyl α-D-mannopyranoside, and manganese dichloride were obtained from Sigma-Aldrich. Tris base, urea (UA), sodium chloride, ammonium bicarbonate (ABC), calcium chloride (CaCl2), and ACS-grade and Optima LC/MS-grade solvents were obtained from Fisher Scientific. Formic acid (FA), 10% sodium dodecyl sulfate solution (SDS), trifluoroacetic acid (TFA), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich. C18 OMIX tips and Phenylboronic acid (PBA) solid-phase extraction cartridges were obtained from Agilent. Hydrophilic interaction chromatography material (PolyHYDROXYETHYL A) was obtained from PolyLC. Microcon filters YM-30 (30 kDa) and amicon Ultra-0.5 ml centrifugal filters (10 kDa) were purchased from Merck Millipore. PANC-1 pancreatic ductal adenocarcinoma cells were from ATCC.
Chen Z., Yu Q., Yu Q., Johnson J., Shipman R., Zhong X., Huang J., Asthana S., Carlsson C., Okonkwo O, & Li L. (2021). In-depth Site-specific Analysis of N-glycoproteome in Human Cerebrospinal Fluid and Glycosylation Landscape Changes in Alzheimer's Disease. Molecular & Cellular Proteomics : MCP, 20, 100081.
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4

Glycoprotein Enrichment and Analysis

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Fetuin from fetal bovine serum, concanavalin A (ConA), wheat germ agglutinin(WGA), Ricinuscommunis agglutinin (RCA120), iodoacetamide (IAA), N-acetyl-D-glucosamine, D-lactose, methyl α-D-mannopyranoside and manganese dichloride were obtained from Sigma-Aldrich (St. Louis, MO). Tris base, urea, sodium chloride, human serum and calcium chloride were obtained from Fisher Scientific (Pittsburgh, PA). C18 OMIX tips were obtained from Agilent (Santa Clara, CA). Dithiothreitol (DTT) and sequencing grade trypsin were supplied from Promega (Madison, WI). Hydrophilic interaction chromatography material was obtained from PolyLC (Columbia, MD).
Yu Q., Wang B., Chen Z., Urabe G., Glover M.S., Shi X., Guo L.W., Kent K.C, & Li L. (2017). Electron-Transfer/Higher-Energy Collision Dissociation (EThcD)-enabled Intact Glycopeptide/Glycoproteome Characterization. Journal of the American Society for Mass Spectrometry, 28(9), 1751-1764.
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5

Multifunctional Nanoparticle Synthesis and Applications

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Cerium (III) nitrate hexahydrate (Ce (NO3)3·6H2O), glucose (Glu), curcumin, alendronate (AL), Tris (2,2-bipyridine) ruthenium chloride (Ru(ddp)), 2-methylimidazole (HMIM), IR780, IR783, IR808, Ce6, Cy3, copper nitrate trihydrate (Cu(NO3)2·3H2O), manganese dichloride (MnCl2), magnesium chloride (MgCl2), cobalt chloride (CoCl2), iron trichloride (FeCl3) and streptozotocin (STZ) were provided by Sigma–Aldrich (USA). Chlorin e6 (Ce6) was obtained from Frontier (UK). glucose oxidase (GOx) and nitrotetrazolium blue chloride (NBT) were purchased from TCI (Japan). Methyl red, 2′,7′-dichlorofluorescin diacetate (DCFH-DA), hematoxylin-eosin (H&E), Masson's trichrome, Sirius staining solution and dihydroethidium (DHE) were purchased from Beyotime Biotechnology (China). Penicillin–streptomycin solution (100X), fetal bovine serum (FBS), trypsin and Dulbecco's modified Eagle's medium (DMEM) and RPMI-1640 medium (1640) were obtained from Gibco (USA). The Cell Counting Kit-8(CCK-8 kit) was purchased from MCE (USA). Anti-CD31 antibody, anti-α-SMA antibody and goat anti-rabbit IgG secondary antibody were purchased from Abcam (UK). High-purity (Milli-Q) water with a resistivity of 18.2 ​MΩ ​cm was obtained from an inline Millipore RiOs/Origin water purification system.
Yu X., Fu X., Yang J., Chen L., Leng F., Yang Z, & Yu C. (2022). Glucose/ROS cascade-responsive ceria nanozymes for diabetic wound healing. Materials Today Bio, 15, 100308.
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