Cells were transfected with 20 nM siRNA targeting RIOK3 (SI02223396; siRIOK3) or AllStars Negative control (1027281; siCon) using HiPerFect reagent (Qiagen, Manchester, UK). Custom siRNA oligonucleotides containing 3 sequences targeting HIF-1α (1 CAAGCAACTGTCATATATA, 2 TGCCACCACTGATGAATTA, 3 TGACTCAGCTATTCACCAA) or HIF-2α (1 TAACGACCTGAAGATTGAA, 2 CAAGCCACTGAGCGCAAAT, 3 TGAATTCTACCATGCGCTA) were purchased from Eurogentec (Southampton, UK). pGIPZ plasmids targeting RIOK3 (Clone ID V2LHS_16875; shRIOK3) or non-silencing control shRNAmir (RHS4743; shCon) were obtained from Thermo Scientific. Lentivirus packaging and titration was carried out as described.38 (link) Cells were transduced at MOI 1 and pools of transduced cells were selected with 1 μg/mL puromycin (Life Technologies) 48 h post-transduction.
Siriok3
Manufactured by Qiagen
Sourced in United Kingdom
The SiRIOK3 is a laboratory instrument designed for the extraction and purification of nucleic acids, such as DNA and RNA, from a variety of biological samples. The core function of the SiRIOK3 is to automate the process of nucleic acid isolation and purification, enabling efficient and consistent results.
Automatically generated - may contain errors
Lab products found in correlation
Hiperfect reagent, by Qiagen (2 mentions)
Puromycin, by Thermo Fisher Scientific (2 mentions)
Si02223396, by Qiagen (2 mentions)
Custom sirna oligonucleotides, by Eurogentec (2 mentions)
Clone id v2lhs 16875 shriok3, by Thermo Fisher Scientific (2 mentions)
Pgipz plasmids, by Thermo Fisher Scientific (2 mentions)
Rhs4743, by Thermo Fisher Scientific (2 mentions)
2 protocols using siriok3
1
Silencing RIOK3 and HIF Transcription
2
Silencing RIOK3 and HIF Transcription
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were transfected with 20 nM siRNA targeting RIOK3 (SI02223396; siRIOK3) or AllStars Negative control (1027281; siCon) using HiPerFect reagent (Qiagen, Manchester, UK). Custom siRNA oligonucleotides containing 3 sequences targeting HIF-1α (1 CAAGCAACTGTCATATATA, 2 TGCCACCACTGATGAATTA, 3 TGACTCAGCTATTCACCAA) or HIF-2α (1 TAACGACCTGAAGATTGAA, 2 CAAGCCACTGAGCGCAAAT, 3 TGAATTCTACCATGCGCTA) were purchased from Eurogentec (Southampton, UK). pGIPZ plasmids targeting RIOK3 (Clone ID V2LHS_16875; shRIOK3) or non-silencing control shRNAmir (RHS4743; shCon) were obtained from Thermo Scientific. Lentivirus packaging and titration was carried out as described.38 (link) Cells were transduced at MOI 1 and pools of transduced cells were selected with 1 μg/mL puromycin (Life Technologies) 48 h post-transduction.
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