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Irtracer 100 spectrophotometer

Manufactured by Shimadzu
Sourced in Germany, Japan

The IRTracer-100 is a Fourier Transform Infrared (FTIR) spectrophotometer manufactured by Shimadzu. It is designed to analyze the infrared absorption spectra of various samples, providing information about their molecular composition and structure.

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13 protocols using irtracer 100 spectrophotometer

1

FTIR Analysis of hIAPP Aggregation

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The FTIR spectra of hIAPP were obtained using a Shimadzu IRTracer-100 spectrophotometer. For this 5 µL of 400 µM hIAPP8–20 (from 0 h to 48 h of incubation time) were placed on a sample holder, air dried and their spectra were acquired between 600 and 4,000 cm−1 at 20 ºC with resolution of 4 cm-1. A blank spectrum was acquired and subtracted from the sample spectra. Peak fitting and secondary structure analysis were performed using PeakFit v4.12 (SeaSolve Software Inc.).
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2

FTIR Analysis of ACP/CHT/TC Composite

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IR active groups of ACP/CHT/TC composite from samples deposited onto IR transparent (Si (100)) was monitored by Fourier transform infrared (FTIR) spectroscopy. Investigations were carried out with a Shimadzu IRTracer-100 spectrophotometer (Shimadzu Europa GmbH, Duisburg, Germany), operating in absorbance mode (for original composite powder and MAPLE coating) and transmittance (for degraded coating) within the (400–4000) cm−1 range, with a 4 cm−1 resolution. Each IR spectrum was obtained as an average of 50 individual scans.
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3

FT-IR Analysis of Purified DPW

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The FT-IR spectrum of the purified DPW was determined using an IRTracer-100 spectrophotometer (Shimadzu (Shanghai) Global Laboratory Consumables Co., Ltd.). In brief, 2 mg of purified DPW fraction was accurately weighed and ground with ~200 mg of dried KBr. The fine powder mixture was then pressed into a pellet and scanned (400–4000 cm−1). The potassium bromide slice without the DPW sample was used as a control.
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4

Characterization of Magnetic Nanoparticles by FT-IR

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Fourier transform infrared (FT-IR) spectroscopy was used to identify the organic components from the composition of the magnetic nanoparticles. Dried powders were ground with potassium bromide (KBr) and further examined in reflection mode using a Shimadzu IRTracer-100 Spectrophotometer (Shimadzu Europa GmbH, Duisburg, Germany). The spectra were recorded in the (5000–500) cm−1 wavelength range at a resolution of 4 cm−1 and averaged over 50 individual scans.
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5

FTIR Analysis of hIAPP Aggregation

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The FTIR spectra of hIAPP were obtained using a Shimadzu IRTracer-100 spectrophotometer. For this, 5 μL of 100 µM NACore w/o C60, C60(OH)24 and C60(OH)40 (100 µM) from 0 to 48 h of incubation time were placed on a sample holder, air dried, and their spectra were acquired between 600 and 4,000 cm−1 at 20 °C with resolution of 4 cm−1. A blank spectrum was acquired and subtracted from the sample spectra. Peak fitting and secondary structure analysis were performed using PeakFit v4.12 (SeaSolve Software Inc.).
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6

Synthesis and Characterization of H/Cu/RGO Nanocomposite

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The synthesis H/Cu/RGO nanocomposite was determined using ultraviolet-visible (UV-vis) spectroscopy (UV-2600, SHIMADZU) in the range of 220–800 nm. X-ray diffraction (XRD, Philips, X’pert, Cu Ka) was used to analyze the structure of the H/Cu/RGO nanocomposite in the range of 5°–80° (2θ). The size and the shape of the nanocomposite were evaluated by using high-resolution transmission electron microscopy (HRTEM, JEM-2100F). Fourier transform infrared (FTIR) spectra were obtained using an attenuated total reflectance (ATR) IRTracer-100 spectrophotometer (Shimadzu, Malaysia). The spectra were set within a range of 400–4,000 cm−1.
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7

ATR-FTIR Spectral Analysis in MIR Region

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ATR-FTIR spectra in the MIR region (400–4000 cm−1) were obtained using an IRTracer-100 spectrophotometer with a QATR that holds a diamond ATR system (Shimadzu, Kyoto, Japan). The resolution was 4 cm−1, while 100 scans over the selected wavenumber range were averaged for each sample. All infrared spectra were collected using LabSolution IR software (version 1.86 SP2, Shimadzu, Kyoto, Japan).
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8

Comprehensive Characterization of BaCO3 Particles

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The morphology and size of the obtained BaCO 3 particles were characterized using a scanning electron microscope (SEM), Quanta 200 (FEI, Netherlands), and a transmission electron microscope (TEM), Zeiss Libra 200F (Carl Zeiss, Germany). The crystalline phase of particles was revealed using powder X-Ray diffraction (PXRD) analysis equipment (Shimadzu 7000maxima X-ray diffractometer). The Fourier transform infrared (FTIR) spectroscopy peaks were obtained using a Shimadzu IRTracer-100 spectrophotometer. The hydrodynamic diameters (D h ) of the BaCO 3 particles were measured using a Zetasizer Nano ZS90 analyzer (Malvern, USA). Energy dispersive X-ray analysis (EDX) was performed using an X-MAX energydispersive X-ray detector (Carl Zeiss, Germany). The details are presented in the ESI. †
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9

Characterization of Magnetic Nanocomposites

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The synthesized Fe3O4@-CD NPs and PLA/ Fe3O4@-CD nanofibrous nanocomposite were characterized using a variety of techniques,
including scanning electron microscopy (SEM, model: Mira 3-TESCAN), X-ray spectroscopy (EDS, model SAMx), X-ray diffraction, and Fourier-transform infrared spectroscopy (FTIR).
SEM was used to assess the size and surface morphology of Fe3O4@-CD nanoparticles and nanofibers.
Image J software (National Institutes of Health version 1.48v) was used to quantitatively assess the high-resolution SEM pictures of the samples.
Crystallite sizes of Fe3O4@β-CD nanoparticles were recorded using a powder X-ray diffractometer (PW3064, Philips, Cu Ka radiation (λ 1.54 Å) with a
scanning range (2ɵ) from 4° ~ 90°.
The elemental analysis was assessed by EDS at an accelerating voltage of 100 kV. Shimadzu’s IRTracer-100 spectrophotometer was used to perform FTIR at a wavenumber of 4000-400 cm1.
The magnetic property of Fe3O4@β-CD NPs and PLA/Fe3O4@β-CD/F. angulata extract nanofibrous nano-composite was
studied by VSM technique using LBKFB device from “Magnetic Kavir Kashan” with a maximum magnetic field of 2.0 T at 298 K.
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10

Characterization of rGO-MnO2 Nanocomposites

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XRD analysis (XRD-Shimadzu 6000 powder diffractometer, Cu-K alpha radiation, 40 kV and 30 mA) was performed for the nanocomposite characterization of rGO decorated with manganese dioxide. The morphology of the nanocomposites was analyzed by scanning electron microscopy (SEM Zeiss EVO MA|10) at 20 kV and a working distance of 8 mm. The images were captured with a secondary electron detector. The dispersed nanocomposite was characterized by transmission electron microscopy (TEM Hitachi, model HT7700 at 120 kV). For qualitative composition analysis, the energy-dispersive spectroscopy (EDS) method (Penta Precision, Oxford Instrument X-act) was used. Moreover, the structural analysis was performed by RAMAN spectroscopy, provided by the WITEC alpha 300 RA, equipped with 532 nm and 785 nm lasers, and Fourier transform analysis, using the IRTracer 100 spectrophotometer (Shimadzu).
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