HIP, WT and AKO rat brains were analyzed by staining with Prussian blue and Luxol fast blue (LFB). For Prussian blue staining, sections were microwaved in solution containing 5% HCl and 5% potassium ferrocyanide (14459-95-1, Acros, Geel, Belgium). Nuclei were stained with neutral red (N7005, Sigma, MO). For Luxol fast blue staining, sections were treated with luxol fast blue (AC212170250, Acros, Geel, Belgium) solution O/N at 56°C, followed by 95% ethanol and dH2O rinse. Slides were differentiated in lithium carbonate solution then 70% ethanol, followed by H&E staining (BP2424-25, E511-100, Fisher, PA). Images were obtained on the Nikon Eclipse 55i upright microscope (Nikon).
N7005
Manufactured by Merck Group
Sourced in Macao
N7005 is a laboratory centrifuge designed for general-purpose applications. It can be used to separate and isolate various biological samples, including cells, proteins, and other biomolecules. The centrifuge operates at adjustable speeds up to 15,000 rpm, providing efficient separation and concentration of sample components. The device is constructed with high-quality materials and features a user-friendly interface for easy operation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using n7005
1
Histochemical Analysis of Rat Brains
2
Cytotoxicity and Cell Viability Assays
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Cell stress was assessed by LDH release using the Roche Cytotoxicity Detection Kit (Roche 11644793001) in cells treated with OxPC (0-160 µg/mL) or PSPC (160 µg/mL) for 24 hours. Culture media was collected, gently centrifuged (150 × RCF for 4 minutes) and combined with the kit reagent for 10 min before absorbance readings were taken at 490 nm. LDH release was expressed as the percentage of LDH activity in the media relative to untreated control cells lysed with 2% triton X-100 (Sigma X-100).
Cell viability was determined by the uptake and retention of neutral red dye. After 24-hour exposure to OxPC or PSPC, a 0.33% stock solution of neutral red (Sigma N7005) in PBS was added 1:20 to the culture media and incubated for 2 hours. Cells were then gently rinsed with PBS and lysed in four times the original culture media volume with a solution of 1% acetic acid and 50% ethanol in H 2 O. Net absorbance was then calculated as A 540 minus A 690 .
Cell viability was determined by the uptake and retention of neutral red dye. After 24-hour exposure to OxPC or PSPC, a 0.33% stock solution of neutral red (Sigma N7005) in PBS was added 1:20 to the culture media and incubated for 2 hours. Cells were then gently rinsed with PBS and lysed in four times the original culture media volume with a solution of 1% acetic acid and 50% ethanol in H 2 O. Net absorbance was then calculated as A 540 minus A 690 .
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