Cp 154526

Manufactured by Bio-Techne

Sourced in United Kingdom

The CP 154526 is a laboratory equipment product manufactured by Bio-Techne. It serves a core function in laboratory applications, but a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

8 protocols using cp 154526

Preparing CRF-R1 Antagonist Solution

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The CRF-R1 antagonist, CP-154,526, was purchased from Tocris Bioscience and dissolved in artificial cerebrospinal fluid (aCSF) at a concentration of 10 μM. The concentration of CP-154,526 was chosen according to previous work from our group (Sotomayor-Zarate et al., 2015 (link)).

Novoa J., Rivero C.J., Pérez-Cardona E.U., Freire-Arvelo J.A., Zegers J., Yarur H.E., Santiago-Marerro I.G., Agosto-Rivera J.L., González-Pérez J.L., Gysling K, & Segarra A.C. (2021). Social isolation of adolescent male rats increases anxiety and K+-induced dopamine release in the nucleus accumbens: Role of CRF-R1. The European journal of neuroscience, 54(3), 4888-4905.

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Pharmacological Manipulation of CRF2 Signaling

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Sprague-Dawley rats were purchased from Harlan Laboratories. CRF₂ homozygous KO mice (Stock number: 010842; Strain name: B6; 129-crhr2^tm1jsp/J) and WT mice (from the same colony) were bought from Jackson Laboratories. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Committee on the Ethics of Animal Experiments of the University of North Dakota (0702-2). All efforts were made to minimize suffering. CRF was purchased from American Peptide Company (Sunnyvale, CA). The following reagents were products of TOCRIS (Ellisville, MO): K41498, astressin 2B, NBI 27914, CP 154526, MDL 12330A, SQ 22536, forskolin, 3,7-dihydro-1-methyl-3-(2-methylpropyl)-1H-purine-2,6-dione (IBMX), KT 5720, Rp-cAMPS. The other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).

Kurada L., Yang C, & Lei S. (2014). Corticotropin-Releasing Factor Facilitates Epileptiform Activity in the Entorhinal Cortex: Roles of CRF2 Receptors and PKA Pathway. PLoS ONE, 9(2), e88109.

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Establishing Alcohol Drinking Solution for Mice

Cited 2 times

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Alcohol for mouse drinking solution (20% v/v) was prepared using 95% ethyl alcohol (Pharmco Products Inc., Brookfield, CT) in tap water. DNQX, AP-5, CGP55845A, CRF peptide, and Astressin-2B were purchased from Tocris Bioscience (Ellisville, MO). SR-95531 (gabazine, GBZ; 100 μM) was purchased from Sigma-Aldrich. The CRF1 antagonist R121919 was supplied by Neurocrine Biosciences, Inc. (San Diego, CA). Stock solutions were prepared in ultra-pure water or DMSO (DNQX only), stored at 20°C, and diluted to final experimental concentration in aCSF on the day of testing. Doses for electrophysiology experiments were chosen based on their ex vivo effects in CeA neurons from prior reports (10 (link), 25 ). CP-154,526 was purchased from Tocris Bioscience and was selected for use in behavioral pharmacology experiments due to a robust literature describing its effects on alcohol drinking in mice (32 (link)–34 (link)).

Agoglia A., Zhu M., Quadir S., Bluitt M., Douglass E., Hanback T., Tella J., Ying R., Hodge C, & Herman M. (2021). Sex-specific plasticity in CRF regulation of inhibitory control in central amygdala CRF1 neurons after chronic voluntary alcohol drinking. Addiction biology, 27(1), e13067.

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Multimodal Regulation of Neuronal Signaling

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Bisindolylmaleimide (BIS; PKC antagonist), PMA (PKC activator), forskolin (adenyly cyclase activator), and H89 [protein kinase A (PKA) antagonist] were obtained from Sigma-Aldrich Co. CRF and CP 154526 were obtained from Tocris Biosciences. Di-4-ANEPPS stock solution was prepared in alcohol and cremophore-EL solution, which can be stored at 4°C for two months. FBS and ACSF were added on the day of the experiment. CRF stock was dissolved in HBSS and Milli-Q water mixture. forskolin, H89, BIS, antalarmin, and PMA stock solutions were prepared in dimethyl sulfoxide (DMSO). All the stock solutions were made 1000 times more concentrated than working concentrations.

Narla C., Jung P.S., Bautista Cruz F., Everest M., Martinez-Trujillo J, & Poulter M.O. (2019). CRF Mediates Stress-Induced Pathophysiological High-Frequency Oscillations in Traumatic Brain Injury. eNeuro, 6(2), ENEURO.0334-18.2019.

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CRF Receptor Antagonists and Cell Death

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Six-well plate cultures of either the C-20/A4 cell line or AC were treated for 8 h with individual or combination treatments of: the CRF-R1 specific antagonist CP-154526 (1–50 μM; Tocris), the CRF-R2 specific antagonist astressin 2B (1–50 μM; Tocris), the non-selective cation channel blocker Gd³⁺ (100 μM; Tocris), the adenylate cyclase activator forskolin (0.1 μM; Tocris), the PLC activator m3M3FBS (0.1 μM; Tocris), or the PLA₂ inhibitor OBAA (0.1 μM; Tocris). Cells were then assayed for cell death or photographed using an Olympus IX73 phase contrast microscope and camera.

Lawrence K.M., Jones R.C., Jackson T.R., Baylie R.L., Abbott B., Bruhn-Olszewska B., Board T.N., Locke I.C., Richardson S.M, & Townsend P.A. (2017). Chondroprotection by urocortin involves blockade of the mechanosensitive ion channel Piezo1. Scientific Reports, 7, 5147.

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Pharmacological Interventions in Animal Model

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CP154,526 and TCS1102 (Tocris Bioscience, Bristol, UK) were dissolved in 100% dimethylsulfoxide (DMSO; Sigma Aldrich, St. Louis, MO, USA) at doses of 0.6 μg/0.5 μl and 15 μg/0.5 μl, respectively. Control vehicle (VEH)-treated animals received 100% DMSO only.

Flores-Ramirez F.J., Matzeu A., Sánchez-Marin L, & Martin-Fardon R. (2022). Blockade of corticotropin-releasing factor-1 receptors in the infralimbic cortex prevents stress-induced reinstatement of alcohol seeking in male Wistar rats: Evidence of interaction between CRF1 and orexin receptor signaling. Neuropharmacology, 210, 109046.

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Neuroactive Substances Delivery Protocol

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Orexin-A (American Peptide) was diluted in 0.9% sodium chloride (Hospira) and injected in volumes of 0.5 μL. Cocaine (National Institute on Drug Abuse) was dissolved in 0.9% sodium chloride and administered at a dose of 0.25 mg/0.1 mL. Sweetened condensed milk (Nestle) was diluted 2:1 (vol./vol.) in water and delivered in volumes of 0.1 mL. Muscimol and baclofen (Tocris Bioscience) were dissolved in 0.9% sodium chloride at concentrations of 0.6 and 0.06 mM, respectively. Finally, CP154526 (Tocris Bioscience) was dissolved in 100% dimethylsulfoxide (Sigma Aldrich).

Matzeu A, & Martin-Fardon R. (2021). Blockade of corticotropin-releasing factor receptor 1 in the central amygdala prevents cocaine-seeking behaviour induced by orexin-A administered to the posterior paraventricular nucleus of the thalamus in male rats. Journal of Psychiatry & Neuroscience : JPN, 46(4), E459-E471.

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Characterization of Pharmacological Compounds

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2-Bromo-α-ergocryptine methanesulfonate salt (BRM) was purchased from Sigma-Aldrich. Aprepitant was obtained from Selleck. All the other compounds tested were purchased from Tocris Biosciences, including SCH 23390 hydrochloride, rotigotine hydrochloride, sumanirole maleate, B-HT 920, Ro 10-5824 dihydrochloride, YM 202074, cinnabarinic acid, MTP, ICI 118,551 hydrochloride, GS 6201, PSB 1115, SC 19220, CP 154526, L-733060, CP 96345, TAM, DOX, A 412997 dihydrochloride, AMN 082 dihydrochloride, SKF 97541, Rac BHFF, SEW 2871, purmorphamine, (R)-(−)-α-methylhistamine dihydrobromide, methimepip dihydrobromide, VU 0155041 sodium salt, antalarmin hydrochloride, NBI 35965 hydrochloride, BQ 788 sodium salt, BAY36-7620, 3-MATIDA, MPEP hydrochloride, MRS 1754, SC 51322, SC 19220, and JTE 013.

Chen Y., Palczewska G., Masuho I., Gao S., Jin H., Dong Z., Gieser L., Brooks M.J., Kiser P.D., Kern T.S., Martemyanov K.A., Swaroop A, & Palczewski K. (2016). Synergistically acting agonists and antagonists of G protein–coupled receptors prevent photoreceptor cell degeneration. Science signaling, 9(438), ra74.

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