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2 protocols using curdione

1

Evaluating Compounds in Hematology

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Normal and abnormal ZTOI were provided by the manufacturer. The standard compounds of β‐elemene, germacrone, curdione and furanodiene were purchased from Shanghai Yuanye Bio‐Technology Co., Ltd. Human Hb and dimethyl sulphoxide (DMSO) were purchased from Sigma‐Aldrich (St. Louis, MO, USA).
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2

Curdione and Bleomycin Induced Fibrosis Study

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Curdione was purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China), and Bleomycin was obtained from MedChemExpress LLC (NJ, USA). Antibodies against β-actin, Gapdh, α-SMA, fibronectin, collagen I, p-Jnk, Jnk, p-p38, p38, p-Erk1/2, Erk1/2, p-Smad2, p-Smad3, Smad2/3 were purchased from Cell Signal Technology Inc. (MA, USA). BCA Protein Assay Kit was supplied by Boster Biological Technology co. Ltd. (Wuhan, China). RT-PCR assay kit was obtained from Takara (Dalian, China). Hydroxyproline assay kit was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Cell Counting Kit-8 was available from DojindoChemical technology co. LTD (Shanghai, China).
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