Live cell imaging medium

Manufactured by Thermo Fisher Scientific

Live Cell Imaging medium is a specialized cell culture medium designed to support the long-term viability and optimal growth of cells during live cell imaging experiments. It provides the necessary nutrients, buffers, and environmental conditions to maintain cells in a healthy state while they are being imaged over extended periods.

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Lab products found in correlation

Bodipy fl pepstatin a, by Thermo Fisher Scientific (1 mentions) Glass bottom culture dishes, by MatTek (1 mentions)

Spelling variants of this product

FluoroBrite DMEM live-cell imaging medium (5 citations) Cell imaging medium (3 citations)

2 protocols using live cell imaging medium

Quantifying Lysosomal Activity in Kidney Cells

Cited 1 time

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The detection of lysosomal activity was performed in live in kidney cells by using Bodipy-FL-Pepstatin A (P12271, Thermo Fischer Scientific) according to the manufacturer’s specifications. The cells were pulsed with 1 μM Bodipy-FL-Pepstatin A in Live Cell Imaging medium (A14291DJ, Thermo Fischer Scientific) for 1 h at 37 °C, fixed, and subsequently analysed by confocal microscopy^{46 (link)}. The numbers of PepA-positive structures per cell were quantified by using the open-source cell image analysis software CellProfiler^TM as described below.

Luciani A., Schumann A., Berquez M., Chen Z., Nieri D., Failli M., Debaix H., Festa B.P., Tokonami N., Raimondi A., Cremonesi A., Carrella D., Forny P., Kölker S., Diomedi Camassei F., Diaz F., Moraes C.T., Di Bernardo D., Baumgartner M.R, & Devuyst O. (2020). Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency. Nature Communications, 11, 970.

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Visualizing SOCS2 Dynamics in GHR Mutants

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HEK293 cells transduced with WT GHR or GHRP495T (GFP-tagged) were seeded on glass-bottom culture dishes (MatTek Corporation, Ashland, MA, USA) and transfected with SOCS2-mCherry 24 h before visualisation on a TIRF microscope (Marianas, SDC EverestTM, Intelligent Imaging Innovations Inc., Denver, CO, USA) fitted with a 100x oil immersion objective (NA=1.46, Carl Zeiss, Jena, Germany) using EMCCD cameras (QuantEM 512sc) and Slidebook software version 5.5 (3i Inc., Denver, CO, USA). Cells were imaged in live-cell imaging medium (Thermo Fisher Scientific) and analysis performed as previously described.^{64 (link)}

Chhabra Y., Wong H.Y., Nikolajsen L.F., Steinocher H., Papadopulos A., Tunny K.A., Meunier F.A., Smith A.G., Kragelund B.B., Brooks A.J, & Waters M.J. (2017). A growth hormone receptor SNP promotes lung cancer by impairment of SOCS2-mediated degradation. Oncogene, 37(4), 489-501.

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