The proliferation of the transfected cells in each group was determined by using the MTS solution cell proliferation assay kits (Promega Corporation, WI, USA) according to the manufacturer’s instructions. The MTS assays were performed on the four consecutive time points (0 h, 24 h, 48 h and 72 h). The absorbance at 490 nm was read by SpectraMax M2 microplate reader (Molecular Devices, LLC, CA, USA), and then the cell proliferation curve was established. The experiment was independently executed three times.
Mts solution cell proliferation assay kits
Manufactured by Promega
Sourced in United States
The MTS solution cell proliferation assay kits are laboratory products designed to measure cell viability and proliferation. The kits utilize a tetrazolium compound that is reduced by metabolically active cells, generating a colorimetric signal that can be quantified. This provides a direct measure of the number of viable cells in a sample.
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2 protocols using mts solution cell proliferation assay kits
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Cell Proliferation Assay Utilizing MTS
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MTS-Based Cell Proliferation Assay
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The MTS assays were performed to provide a preliminary assessment of toxicity of the inducing agents. The proliferation of the cells in each group was detected using the MTS solution cell proliferation assay kits (Promega Corporation, WI, USA). The MTS assays were performed at days 1, 3, 5, and 7. The absorbance at 490 nm was read using a SpectraMax M2 microplate reader (Molecular Devices, LLC, CA, USA), and the final absorption (A) values were the values in each group minus the background absorbance values. The cell proliferation curve was then established.
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