1 2 dipalmitoyl rac glycero 3 phosphocholine

Manufactured by Merck Group

Sourced in Germany

1,2-dipalmitoyl-rac-glycero-3-phosphocholine is a synthetic phospholipid compound. It is commonly used as a research tool and in the development of liposomal drug delivery systems.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using 1 2 dipalmitoyl rac glycero 3 phosphocholine

Lipid Quantification and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Solvents and chemical reagents used were of reagent grade and purchased from Merck (Darmstadt, Germany). The following solvents were employed: ethanol, acetone, ethyl acetate, chloroform, methanol, hexane, and toluene. The following reagents were used: ammonium molybdate, cupric acetate, pyridine, acetic acid, acetic anhydride, acetyl chloride, NaCl, anhydride sodium sulphate, sulphuric acid, ferric trichloride, perchloric acid, nitric acid, and hydroxylamine.
Quantitative standards (1,2-dipalmitoyl-rac-glycero-3-phosphocholine, oleic acid, cholesterol, methyl stearate, nonadecanoic acid) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Qualitative (Qualmix Fish) and quantitative (FAME Mix) FAME standards were obtained from Supelco, Inc. (Bellefonte, PA, USA) and Larodan (Malmo, Sweden), respectively.

Aubourg S.P., Trigo M., González M.J., Lois S, & Medina I. (2022). Comparative Study of Bioactive Lipid Extraction from Squid (Doryteuthis gahi) by-Products by Green Solvents. Foods, 11(15), 2188.

+ Open protocol

+ Expand

Fabrication of Lipid-Magnetic Nanovesicles

Check if the same lab product or an alternative is used in the 5 most similar protocols

25 mg of 1-stearoyl-rac-glycerol (Sigma-Aldrich), 2.5 mg of oleic acid (Sigma-Aldrich), 2.5 mg of 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (Sigma-Aldrich), 4 mg of mPEG-DSPE_5k (Sigma-Aldrich), and 2.5 mg of temozolomide (Sigma-Aldrich) (when TMZ-LMNVs are fabricated), are mixed with 84.5 μl of an ethanol solution of superparamagnetic iron oxide nanoparticles (15 wt%; US Research Nanomaterials Inc.), inside a 6 ml glass vial. Subsequently, the vial is placed inside an ultrasonic bath (Elmasonic S 35w) set at 70 °C in order to melt the lipids and to allow ethanol to evaporate. After ethanol evaporates, 3 ml of a pre-warmed (70 °C) Tween® 80 (Sigma-Aldrich) solution (1.0 wt%) were added to the lipid mixture and immediately sonicated using an ultrasonic homogenizer (Fisherbrand™ Q125 Sonicator) for 15 min (amplitude 30%, 120 W). After the ultrasonic homogenization, the hot mixture is transferred to a high pressure homogenizer (HPH, EmulsiFlex-B15 from Avestin), where the sample is further homogenized by passing it 5 times through the homogenizer at a pressure of 100 000 psi. After the homogenization, the LMNVs are placed for 30 min at 4 °C to allow the lipid-based structures to stabilize. The LMNVs are purified by centrifugation and washing with ultrapure (Mili-Q) water (3 times for 30 min at 4 °C).

Tapeinos C., Marino A., Battaglini M., Migliorin S., Brescia R., Scarpellini A., De Julián Fernández C., Prato M., Drago F, & Ciofani G. (2018). Stimuli-responsive lipid-based magnetic nanovectors increase apoptosis in glioblastoma cells through synergic intracellular hyperthermia and chemotherapy †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8nr05520c. Nanoscale, 11(1), 72-88.

+ Open protocol

+ Expand

Curcumin-Piperine Liposomal Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Curcumin, piperine, glyceryl tripalmitate (tripalmitin, purity ≥ 99%), 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (DPPC, ∼99%), Cholesterol (≥%99) were purchased from Sigma-Aldrich, Germany. Chloroform (≥99.8%) was obtained from Fluka Chemika, Germany. Dimethyl sulfoxide (DMSO) was purchased from Fisher BioReagents, United States. All chemicals were used as received without further purification.
3-(4,5-di-methyl-thiazol-2-yl)-5-(3-carboxy-methoxy- phenyl)-2-(4-sulfo-phenyl)-2H-tetrazolium (MTS)-assay (CellTiter96 AqueousOne Solution) was purchased from Promega, Southampton, United Kingdom. Annexin V-FITC Apoptosis Detection Kit was obtained from BD Pharmingen. Propidium iodide and RNase A were purchased from Sigma-Aldrich, Germany. Non-idet P-40 was obtained from AppliChem, Germany. 4,6-Diamidino-2-phenylindole dihydrochloride (DAPI) was purchased from Roche. pKH26 was obtained from Sigma-Aldrich, Germany.

Bolat Z.B., Islek Z., Demir B.N., Yilmaz E.N., Sahin F, & Ucisik M.H. (2020). Curcumin- and Piperine-Loaded Emulsomes as Combinational Treatment Approach Enhance the Anticancer Activity of Curcumin on HCT116 Colorectal Cancer Model. Frontiers in Bioengineering and Biotechnology, 8, 50.

+ Open protocol

+ Expand

Curcumin-Loaded Lipid Nanocarriers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Curcumin, glyceryl tripalmitate (tripalmitin, purity ≥99%), 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (DPPC, ∼99%), glutaraldehyde solution (50%), human IgG-Reagent Grade, anti-human IgG (γ-chain specific)-gold antibody, Sudan III and FITC were purchased from Sigma–Aldrich GmbH, Germany. Hexadecylamine (HDA, ≥99%), uranyl acetate dehydrate (≥98%) and chloroform (≥99.8%) were obtained from Fluka Chemika, Switzerland and Germany, respectively. Cholesterol (>%98) was purchased from Avanti Polar Lipids, USA. Dimethyl sulfoxide (DMSO) was purchased from Riedel-de Haën (Sigma–Aldrich, Germany) and isopropyl-β-d-thiogalactoside (IPTG) was purchased from Gerbu (Gaiberg, Germany). All chemicals were used as received without further purification.

Ucisik M.H., Küpcü S., Breitwieser A., Gelbmann N., Schuster B, & Sleytr U.B. (2015). S-layer fusion protein as a tool functionalizing emulsomes and CurcuEmulsomes for antibody binding and targeting. Colloids and Surfaces. B, Biointerfaces, 128, 132-139.

+ Open protocol

+ Expand

Lipid-Based Curcumin Delivery System

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glyceryl tripalmitate (tripalmitin, purity ≥99%), 1,2dipalmitoyl-rac-glycero-3-phosphocholine (DPPC, ≥99%), cholesterol (≥%99), hexadecylamine (HDA, ≥99%), curcumin were purchased from Sigma-Aldrich, Germany. Chloroform (≥99.8%) was obtained from Fluka Chemika, Germany. Dimethyl sulfoxide (DMSO) was purchased from Fisher BioReagents, USA. All chemicals were used as received without further purification.

Üçışık M.H. (2021). Replacement of Extrusion by Temperature-Controlled Ultrasonication in Emulsome Production. European Journal of Science and Technology.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!