Sab2103167

Manufactured by R&D Systems

SAB2103167 is a laboratory equipment product offered by R&D Systems. It serves a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation or interpretation.

Automatically generated - may contain errors

Lab products found in correlation

Sab1412231, by R&D Systems (2 mentions) Ab4441, by Abcam (1 mentions) Ab12188, by Abcam (1 mentions) Pp h6939 00, by Abcam (1 mentions)

2 protocols using sab2103167

ChIP and PLA for Epigenetic Regulation

Cited 1 time

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ChIP was performed as previously described [22 (link)]. The antibodies used for ChIP were anti-H3K9Ac (Abcam, #ab4441), anti-KAT2B (Abcam, #ab12188), anti-HNF4A (R&D Systems, PP-H6939-00), anti-IgG (CST, #3900) and anti-HIC1 (Sigma, #SAB1412231). The primers are listed in Supplementary Table S1.
The PLA was performed as previously described [21 (link)]. The primary antibodies used were anti-HIC1 (Sigma, #SAB1412231 or #SAB2103167), anti-HNF4A (R&D Systems, PP-H6939-00) and anti-KAT2B (Abcam, #ab12188).

Zhang X., Du L., Qiao Y., Zhang X., Zheng W., Wu Q., Chen Y., Zhu G., Liu Y., Bian Z., Guo S., Yang Y., Ma L., Yu Y., Pan Q., Sun F, & Wang J. (2019). Ferroptosis is governed by differential regulation of transcription in liver cancer. Redox Biology, 24, 101211.

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Quantitative RT-PCR and Co-Immunoprecipitation

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Quantitative RT-PCR was performed as previously described [21 (link)]. The primers are listed in Supplementary Table S1.
The antibodies used for co-IP were anti-HIC1 (Sigma, #SAB1412231, and #SAB2103167) and anti-HNF4A (R&D Systems, PP-H6939-00), and the conventional co-IP protocols are available elsewhere.

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