All CD14+ monocytes and THP-1 cells were cultured in RPMI 1640 medium (Gibco, New York, USA) supplemented with 10% fetal bovine serum (FBS, Capricorn Scientific, USA). Human umbilical vein endothelial cells (HUVECs) were cultured with DMEM basic (1×) (Gibco, New York, USA) that contained 10% fetal bovine serum. The cultures were incubated at 37 °C in a humidified atmosphere, which contained 5% CO2.
Dmem basic 1
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
DMEM basic (1×) is a basal medium commonly used for the culture of various cell types. It provides essential nutrients and ingredients to support cell growth and maintenance in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Capricorn (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Sartorius (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000 transfection kit, by Thermo Fisher Scientific (1 mentions)
L tryptophan, by Merck Group (1 mentions)
Panc 1 atcc crl 1469, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Atlanta Biologicals (1 mentions)
K sfm, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg hrp antibody, by Solarbio (1 mentions)
Sqstm1 p62, by Abcam (1 mentions)
6 protocols using dmem basic 1
1
Cell Culture Conditions for Monocytes
2
HeLa Cell Culture and Transfection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa cells were cultured in DMEM basic (1×) (Gibco, China, Cat. C11995500BT) supplemented with 10% fetal bovine serum (Biological Industries, Israel, Cat.04-001-1A) and 1% ampicillin–streptomycin (Hyclone, South Logan, Utah). Transfection was performed with the Lipofectamine™ 3000 Transfection Kit (Invitrogen, American, Cat. L3000-001) following the manufacturer’s instruction.
3
Panc-1 PDAC Cell Line Stimulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human PDAC cell line (Panc‐1 ATCC® CRL‐1469™) was purchased from the ATCC. Panc‐1 cells were plated in Dulbecco's Modified Eagle Medium (DMEM) basic (1×) (Gibco). DMEM was supplemented by 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (ATLANTA Biologicals). Next, cells were collected and washed twice with phosphate‐buffered saline (PBS). Then, 1 × 106 cells/well were cultured in a 48‐well plastic plate (Corning Costar). Thereafter, cell culture medium was supplemented by 1 µg/mL l ‐Tryptophan (Sigma‐Aldrich) and incubated for 72 hours. A total of 20 ng/mL of human purified recombinant indoleamine 2,3 dioxygenase1 (hIDO1) (Prospec) was added every 6 hours to stimulate the Kynp in the tumor cells. This culture system was duplicated and repeated three times.
4
Culturing Human Oral Epithelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Raw cells were obtained from the Shanghai Institute of Cell Biology (Shanghai, China) and cultured with DMEM basic (1×) (Gibco, Thermo Fisher Scientific) at 37 °C in 5% CO2 and humidified air incubators.
Leuk-1 cells, an immortalized human oral mucosal epithelial cell line, were a generous gift from Professor Li Mao in the Department of Oncology and Diagnostic Sciences, University of Maryland Dental School, Baltimore, MD, USA. Leuk-1 cells were cultured and passaged in a defined keratinocyte serum-free medium (K-SFM) (Gibco, Invitrogen, Carlsbad, CA, USA).
Leuk-1 cells, an immortalized human oral mucosal epithelial cell line, were a generous gift from Professor Li Mao in the Department of Oncology and Diagnostic Sciences, University of Maryland Dental School, Baltimore, MD, USA. Leuk-1 cells were cultured and passaged in a defined keratinocyte serum-free medium (K-SFM) (Gibco, Invitrogen, Carlsbad, CA, USA).
5
Culturing BHK-21 Cells for SVA Studies
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BHK-21 cells (maintained in our laboratory) were cultured at 37°C 5% CO2 in DMEM basic (1×) (Gibco) supplemented with 10% fetal bovine serum (Biological Industries, Israel). The SVA strain (SVA-CH-SDGT-2017) and an anti-SVA-VP2 monoclonal antibody were prepared in our laboratory. The following antibodies were used: Ago2 (Abcam, ab186733), LC3B (Sigma-Aldrich, L7543), SQSTM1/p62 (Abcam, ab56416), goat Anti-rabbit IgG/HRP antibody (Solarbio, SE134).
6
Culturing Monocytes and HUVECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All CD14+ monocytes were cultured in RPMI 1640 medium (Gibco, USA) supplemented with 10% fetal bovine serum (Capricorn Scientific, USA). Human umbilical vein endothelial cells (HUVECs) were cultured with Dulbecco’s Modified Eagle Medium (DMEM) basic (1×) (Gibco, USA) that contained 10% fetal bovine serum. The cultures were incubated at 37°C in a humidified atmosphere containing 5% CO2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!