Trans, trans-FOH, dimethyl sulfoxide (DMSO), 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO), 3-[2-[2-chloro-4-[[3-(2,6-dichlorophenyl)-5-(1-methylethyl)-4 isoxazolyl]methoxy]phenyl]ethenyl]benzoic acid (GW4064), OA, hematoxylin, Oil Red O (ORO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), triamcinolone acetonide, Triton X-100, and fatty acid-free bovine serum albumin (FF-BSA) were purchased from Sigma-Aldrich (St. Louis, MO). 2-[[4-[2-[[(Cyclohexylamino)carbonyl](4-cyclohexylbutyl)amino]ethyl]phenyl]thio]-2-methylpropanoic acid (GW7647) and N-((2S)-2-(((1Z)-1-methyl-3-oxo-3-(4-(trifluoromethyl)phenyl)prop-1-enyl)amino)-3-(4-(2-(5-methyl-2-phenyl-1,3-oxazol-4 (GW6471) were purchased from Bio-Techne (Minneapolis, MN). Williams’ Medium E, Penicillin-Streptomycin solution, and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA), and recombinant human insulin (Novolin R) from Novo Nordisk Pharmaceuticals, Inc. (Princeton, NJ). Tritiated OA ([9, 10-3H(N)]OA, 15–60Ci (0.555–2.22TBq)/mmol) was purchased from PerkinElmer (Waltham, MA). Other materials were obtained from the sources indicated below.
6 4 chlorophenyl imidazo 2 1 b 1 3 thiazole 5 carbaldehyde o 3 4 dichlorobenzyl oxime
Manufactured by Merck Group
Sourced in United States
6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime is a chemical compound. It has the molecular formula C21H13Cl3N2OS.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel matrix, by Corning (4 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Rifampin, by Merck Group (3 mentions)
Biocoat collagen 1 coated multiwell plates, by Corning (2 mentions)
Triton x 100, by Merck Group (1 mentions)
William s e medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
Fatty acid free bovine serum albumin ff bsa, by Merck Group (1 mentions)
Triamcinolone acetonide, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Nifedipine, by Merck Group (1 mentions)
Dehydronifedipine, by LGC (1 mentions)
Dehydronifedipine d6, by LGC (1 mentions)
Diethylpyrocarbonate depc treated water, by Thermo Fisher Scientific (1 mentions)
Williams e medium, by Merck Group (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Glutamax 1 supplement, by Thermo Fisher Scientific (1 mentions)
Superscript 3 first strand synthesis supermix, by Thermo Fisher Scientific (1 mentions)
High viability cryohepatocyte recovery kit, by Corning (1 mentions)
6 protocols using 6 4 chlorophenyl imidazo 2 1 b 1 3 thiazole 5 carbaldehyde o 3 4 dichlorobenzyl oxime
1
Liver Cell Culture Protocol
2
Evaluating Nuclear Receptor Modulators
3
Cryopreserved Hepatocyte Characterization
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Cryopreserved human hepatocytes from three donors (#1: HMC 514, #2: HFC920, #3: HMC1034), the High Viability Cryohepatocyte Recovery Kit, Hepatocyte Culture Media Kit, Matrigel matrix, and collagen I-coated 24-well plates were obtained from Corning Life Sciences (Woburn, MA, USA). GlutaMAX-I supplement was purchased from Gibco (Grand Island, NY, USA). Cryopreserved HepaRG cells, HepaRG Thaw, Plate, and General Purpose Medium Supplement, and HepaRG Induction Medium Supplement were obtained from Biopredic (Rennes, France). Calcitriol, omeprazole, 6-(4-chlorophenyl)imidazo[2,1-b] [1,3]thiazole-5-carbaldehyde O-3,4-dichlorobenzyl) oxime (CITCO), rifampin, bupropion, and William’s Medium E were purchased from Sigma-Aldrich (St. Louis, MO, USA). Superscript III First-Strand Synthesis SuperMix, TRIzol, and diethyl pyrocarbonate (DEPC)-treated water were obtained from Invitrogen (Carlsbad, CA, USA). Quantitative polymerase chain reaction (qPCR) reagents were purchased from Applied Biosystems (Foster City, CA, USA). Dimethyl sulfoxide (DMSO) was obtained from Merck (Merck-Millipore, Darmstadt, Germany). All other chemicals were of reagent grade or better and were used without further purification.
4
Hormone Receptor Assay Protocol
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Reagents were obtained from Life Technologies Corporation (Carlsbad, CA, United States) unless otherwise indicated. Estrone (E1), estradiol (E2), estriol (E2), P4, CRT, rifampicin (RIF), 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde-O-(3,4-dichlorobenzyl)oxime (CITCO), chenodeoxycholic acid (CDCA), piperazine-N,N′-bis(2-ethanesulfonic acid) (PIPES), phenylmethylsulfonyl fluoride (PMSF), sucrose, and dimethyl sulfoxide (DMSO) were obtained from Sigma Aldrich (St. Louis, MO, United States). Placental growth hormone (pGH) was purchased from R&D Systems (Minneapolis, MN, United States). QualGro™ Seeding, QualGro™ Culture, and QualGro™ Induction media were obtained from BioIVT (Durham, NC); Matrigel Matrix and Corning Biocoat™ collagen I coated plates were purchased from Corning (Corning, NY, United States). Enzyme-linked immunosorbent assay (ELISA) kits for E2, E3, P4, and CRT were purchased from Cayman Chemical (Ann Arbor, MI, United States). Ethylenediaminetetraacetic acid (EDTA) and digitonin were obtained from EMD Millipore (Burlington, MA, United States).
5
Hepatocyte Characterization Assay Protocol
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Omeprazole (OP), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO) and phenobarbital (PB) were purchased from Sigma-Aldrich (St. Louis, MO). Matrigel™ (phenol red free) was obtained from Corning (Bedford, MA). Collagen I coated 96-well plates were obtained from Life Technologies (Carlsbad, CA). The hepatocyte thawing medium (UCRM™) was purchased from In Vitro ADMET (Columbia, MD). The hepatocyte plating medium (InVitroGRO™ CP medium), incubation medium (InVitroGRO™ HI medium) and TorpedoTM Antibiotic Mix were from BioIVT (Westbury, NY). All oligonucleotide primer and probe sets were manufactured by Life Technologies (Table 2 ).
6
Hormone Metabolism Assay Protocol
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All reagents were obtained from ThermoFisher Scientific (Waltham, MA) unless otherwise indicated. Dimethyl sulfoxide (DMSO), labetalol, 17β-estradiol (E2), estriol (E3), estetrol (E4), progesterone (P4), cortisol (CRT), rifampin, and 6-(4-Chlorophenyl) Imidazo [2,1-b][1,3] Thiazole-5-Carbaldehyde o-(3,4-dichlorobenzyl) Oxime (CITCO) were purchased from Sigma-Aldrich (St. Louis, MO). Matrigel® matrix and Biocoat™ Collagen I Coated Multiwell Plates were obtained from Corning (Bedford, MA). labetalol-d3 was purchased from Toronto Research Chemicals (Toronto, ON, Canada).
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