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Alexa fluor 594 goat anti rabbit igg h l antibody

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Alexa Fluor® 594 goat anti-rabbit IgG (H+L) antibody is a secondary antibody conjugated with the red-fluorescent Alexa Fluor® 594 dye. It is designed to detect and visualize rabbit primary antibodies in various immunodetection applications.

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Lab products found in correlation

Unc0638, by Merck Group (2 mentions) Alexa fluor 488 goat anti mouse igg h l antibody, by Thermo Fisher Scientific (2 mentions) Histone h3, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Axiovert 200 base microscope, by Zeiss (1 mentions) Pparγ, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Senescence β galactosidase staining kit, by Beyotime (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) Chloral hydrate, by Merck Group (1 mentions) Fam labeled taqman probe, by Thermo Fisher Scientific (1 mentions) Anti parvalbumin antibody, by Abcam (1 mentions) D amphetamine sulfate, by Merck Group (1 mentions) Bix 01294, by Merck Group (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions) Green fluorescent protein antibody, by Santa Cruz Biotechnology (1 mentions)

4 protocols using alexa fluor 594 goat anti rabbit igg h l antibody

1

Immunofluorescent CD31 Staining Protocol

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For immunofluorescent staining of CD31, CD31 rat anti-mouse mAb (Life Technologies, Inc.), and Alexa Fluor® 594 goat anti-rabbit IgG (H+L) antibody (Life Technologies, Inc.) were used as primary and secondary antibodies, respectively. Briefly, sections were blocked with 10% goat bovine serum albumin for 30 min, followed by overnight incubation at 4 °C with the primary antibody and 45 min at 4 °C with the secondary antibody. After each step slides were washed with PBS. Fluorescent images were taken with a Zeiss Axiovert 200 base microscope.
Chen H., Liu D., Li Y., Xu X., Xu J., Yadav N.N., Zhou S., van Zijl P.C, & Liu G. (2019). CEST MRI monitoring of tumor response to vascular disrupting therapy using high molecular weight dextrans. Magnetic resonance in medicine, 82(4), 1471-1479.
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2

Senescence and DNA Damage Pathway Analysis

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UNC0638, CPT were both purchased from Sigma (St. Louis, MO, USA). Senescence β-Galactosidase Staining Kit, RIPA were purchased from Beyotime (Nan-tong, China). The following antibodies were used as primary antibodies: G9a (Cell Signaling, Danvers, MA, USA), ATM (Cell Signaling), p-Chk1 (Ser 317), p-Chk2 (Thr 68), γH2AX (Santa Cruz Biotechnology, Santa Cruz, CA, USA), Histone H3 (Cell Signaling), Histone H3 Dimethyl (K9) antibody (Epitomics, CA, USA), EpCAM (Epitomics), PPARγ (Cell signaling), Wnt6 (Epitomics), Wnt10a (Epitomics), β-actin (Cell Signaling), GAPDH (Cell Signaling), GFP (Santa Cruz Biotechnology), Alexa Fluor 488 Goat Anti-Mouse IgG (H+L) Antibody (Life Technologies, Oregon, USA), Alexa Fluor 594 Goat Anti-Rabbit IgG (H+L) Antibody (Life Technologies).
Zhang J., He P., Xi Y., Geng M., Chen Y, & Ding J. (2015). Down-regulation of G9a triggers DNA damage response and inhibits colorectal cancer cells proliferation. Oncotarget, 6(5), 2917-2927.
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3

Lentiviral Particle Production and Immunohistochemistry

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High titer lentiviral particles were obtained from Thermo Scientific. Pentobarbital sodium, chloral Hydrate, and D-Amphetamine sulfate were all purchased from Sigma. FAM-labeled TaqMan probes, the secondary AlexaFluor® 594 goat anti-rabbit IgG (H+L) antibody as well as the ProLong gold antifade mountant were obtained from Life Technologies. The anti-parvalbumin antibody was purchased from ABCAM. All other chemicals and reagents were of either analytical or laboratory grade and purchased from various suppliers.
Boley A.M., Perez S.M, & Lodge D.J. (2014). A fundamental role for hippocampal parvalbumin in the dopamine hyperfunction associated with schizophrenia. Schizophrenia research, 157(0), 238-243.
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4

Evaluation of Epigenetic Modulators

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UNC0638 (#U4885) and BIX-01294 (#B9311) were both purchased from Sigma (St. Louis, MO, USA). The iron chelator DFO was from Novarits (Switzerland). FAC was from JK Chemical (#F5879, Shanghai, China). Kits of the membrane and cytosol protein extraction kit (#P0033), the ROS assay (#S0033) and the cell lysis buffer radio-immunoprecipitation assay (#P0013B) were purchased from Beyotime (Nantong, China). Monoclonal antibodies specific for G9a (1:1,000; #3306), YY1 (1:1,000; #2185), HDAC1 (1:1,000; #34589), HDAC2 (1:1,000; #57156), Histone H3 (1:2,000; #4499), K9 dimethylated Histone H3 (1:2,000; #4658), β-actin (1:5,000; #8457) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:5,000; #5174) were purchased from Cell Signaling Inc. (Danvers, MA, USA). The other antibodies were as follows: HEPH antibody (1:500; #sc-365365, Sigma), green fluorescent protein (GFP) antibody (1:1,000; #sc-69779, Santa Cruz Biotechnology), Alexa Fluor 488 goat anti-mouse IgG (H + L) antibody (1:200; #A-11001, Life Technologies, OR, USA) and Alexa Fluor 594 goat anti-rabbit IgG (H + L) antibody (1:200; # A-11012, Life Technologies).
Wang Y.F., Zhang J., Su Y., Shen Y.Y., Jiang D.X., Hou Y.Y., Geng M.Y., Ding J, & Chen Y. (2017). G9a regulates breast cancer growth by modulating iron homeostasis through the repression of ferroxidase hephaestin. Nature Communications, 8, 274.
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