The chemicals used for the preparation of DESs included Betaine (99%), DL-Menthol (≥ 95%), Nile Red (≥ 98%) purchased from Sigma, Lactic acid (85%), Lauric acid (98%), Myristic acid (≥ 98%) obtained from Sigma–Aldrich, L-proline (98%) from Scharlau, D-(+)-glucose anhydrous (≥ 95%), Stearic acid (98%) from Merck/Sigma, Ethanol (96%) from Valente and Ribeiro, Methanol PA from Honeywell, Folin-Ciocalteau Reagent from Panreac, L-Ascorbic acid (99%), Quercetin (HPLC grade), which were purchased from Sigma and were used as purchased.
Folin ciocalteau reagent
Manufactured by ITW Reagents
Sourced in Spain
The Folin-Ciocalteau reagent is a chemical solution used in colorimetric assays for the determination of phenolic compounds. It functions by reacting with phenolic groups, producing a blue-colored complex that can be measured using spectrophotometry.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Merck Group (2 mentions)
Quercetin 3 o glucoside, by Extrasynthese (2 mentions)
Methanol, by ITW Reagents (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions)
Phenol, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Sodium carbonate, by ITW Reagents (2 mentions)
Nile red, by Merck Group (1 mentions)
D glucose anhydrous, by Merck Group (1 mentions)
Lactic acid, by Merck Group (1 mentions)
Stearic acid, by Merck Group (1 mentions)
Myristic acid, by Merck Group (1 mentions)
Lauric acid, by Merck Group (1 mentions)
L ascorbic acid, by Merck Group (1 mentions)
Dl menthol, by Merck Group (1 mentions)
Xylose, by Merck Group (1 mentions)
Ethanol, by ITW Reagents (1 mentions)
4 hydroxybenzoic acid, by Merck Group (1 mentions)
Procyanidin b2, by Extrasynthese (1 mentions)
Quercetin 3 o glucuronide, by Extrasynthese (1 mentions)
5 protocols using folin ciocalteau reagent
1
Preparation and Characterization of Deep Eutectic Solvents
2
HPLC Analysis of Polyphenolic Compounds
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Acetonitrile HPLC quality was supplied by Labscan (Dublin, Ireland), and formic acid HPLC quality was from Acros Organic (Geel, Belgium). Protocatechuic acid, vanillic acid, syringic acid, caffeic acid, 3-coumaric acid, ethyl gallate, 3,4′,5 trihydroxystilbene-3-O-d -glucoside (trans-piceid), (+)-catechin, (−)-epicatechin, epicatechin gallate, procyanidin B1, procyanidin B2, quercetin-3-O-galactoside (hyperoside), quercetin-3-O-rutinoside (rutin), quercetin-3-O-glucuronide, quercetin-3-O-glucoside, and quercetin dehydrate were purchased from Extrasynthèse (Genay, France). Glucose, xylose, phenol, gallic acid, 4-hydroxybenzoic acid, trans-caftaric acid, ellagic acid, protocatechuic aldehyde, trans-resveratrol, 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), potassium persulfate, 2,2´ azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 2,2 diphenyl-1-picrylhydrazyl (DPPH), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) were obtained from Sigma-Aldrich (Madrid, Spain). Disodium carbonate, Folin-Ciocalteau reagent, methanol, and ethanol were purchased from Panreac (Barcelona, Spain).
3
Comprehensive Characterization of Reagents
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All reagents used for characterization were analytical grade. Sulfuric acid, phenol, and iron sulfate were supplied by Merck (Darmstadt, Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris (2-pyridyl)-S-triazine (TPTZ), potassium hydroxide, potassium chloride, iron (III) chloride, sodium chloride, potassium dihydrogen phosphate, sodium phosphate, sodium azide, phloroglucinol, and sodium acetate were from Millipore Sigma (St. Louis, MO, USA). 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), sodium carbonate, Folin–Ciocalteau reagent, chlorohydric acid, glucose, Trolox, sodium hydroxide, and methanol were from Panreac (Barcelona, Spain).
4
Antioxidant Evaluation of Chitosan-Based Biomaterials
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Crude FFv, CS (low molecular weight, 75–85% deacetylation degree), gelatin from porcine skin (type A), barium chloride, trichloroacetic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), glacial acetic acid, Dulbecco’s Modified Eagle’s (DMEM), L-glutamine, non-essential amino acids, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (Burghausen, Germany). Potassium sulphate (K2SO4) and Folin-Ciocalteau reagent were from Panreac AppliChem (Barcelona, Spain). 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonate) (ABTS) was supplied by Alfa Aesar (Kandel, Germany), and sodium carbonate (Na2CO3) was purchased from Merck (Darmstadt, Germany). Phloroglucinol was supplied by AcrosOrganics (Shanghai, China). Polystyresulfonate sodium salt (PSS) standards were acquired from Sigma and Fluka (Barcelona, Spain and St. Louis, MO, USA, respectively). Phosphate-buffered saline (PBS) tablets pH 7.4 were acquired from VWR (Rosny-sous-Bois, France) and dimethyl sulfoxide (DMSO) from Carlo Erba (Sabadell, Spain). Penicillin/streptomycin and fetal bovine serum (FBS) were provided by Gibco (Life Technologies, Carlsbad, CA, USA), while the lactate dehydrogenase (LDH) kit was purchased from Takara Bio (Tokyo, Japan). Ultrapure water (Milli-Q, Millipore, Watford, UK) was used throughout. All other chemicals were reagent grade.
5
Antiproliferative Effects of Phenolic Compounds
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Methanol, n-hexane and hydrochloric acid were purchased from Labscan (Dublin, Ireland). Formic acid, HPLC-grade acetonitrile, sodium carbonate, and Folin-Ciocalteau reagent were obtained from Panreac (Barcelona, Spain). Gallic acid, protocatechuic acid, (+)-catechin, (À)-epicatechin, quercetin, kaempferol, quercetin-3-O-rutinoside (rutin), ferulic acid, caffeic acid, p-coumaric acid, and phosphate buffered saline (PBS) were purchased from Sigma-Aldrich (Madrid, Spain). quercetin-3-O-glucoside and kaempferol-3-O-glucoside were obtained from Extrasynthese (Genay, France). Alamar Blue was from Abd Serotec (Kidlington, UK). Fehling's reagents, dimethyl sulfoxide (DMSO), sulforhodamine B-based assay kit and all other reagents used for assay with cells were obtained from Sigma Aldrich (UK).
The stock solutions of phenolic standards were prepared in water at concentration of 100 mg/L. The corresponding dilutions were made up from the stock solutions to assess the antiproliferative effects.
The stock solutions of phenolic standards were prepared in water at concentration of 100 mg/L. The corresponding dilutions were made up from the stock solutions to assess the antiproliferative effects.
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