2 × 106 freshly isolated mouse splenocytes, resuspended in PBS with 2% FBS, were labeled for 30 min at 4°C in the dark with different antibody panels. The antibodies used were: α-CD8 (5H10, Thermo Fisher, cat. nr. MCD0828TR), α-CD4 (RM4-5, Thermo Fisher, cat. nr. 56-0042-80), α-CD3 (17A2, eBioscience, cat. nr. 46-0032-80), α-CD62L (MEL-14, Biolegend, cat. nr. 104411), α-CD44 (IM7, Biolegend, cat. nr. 103005), α-PD-1 (29F-1A12, Biolegend, cat. nr. 135223), α-IL7R (A7R34, Biolegend, cat. nr. 135031), α-CTLA-4 (UC10-4B9, eBioscience, cat. nr. 12-1522-81), α-B220 (RA3-6B2, eBioscience, cat. nr. 47-0452-82), α-CD21 (eBio4E3, eBioscience, cat. nr. 48-0212-80), α-CD23 (B3B4, Biolegend, cat. nr. 101612), α-CD86 (GL1, eBioscience, cat. nr. 12-0862-81), α-CD69 (H1.2F3, eBioscience, cat. nr. 11-0691-81), α-IAb (AF6-120.1, eBioscience, cat. nr. 46-5320-80). The cells were then washed twice with PBS, resuspended in PBS with 2% FBS and data were acquired on an LSRII Flow Cytometer (BD Biosciences) and analyzed with FlowJo software, ver. 9.9.6 (Treestar, Inc).
Af6 120
Manufactured by Thermo Fisher Scientific
Sourced in United States
The AF6-120.1 is a laboratory equipment product from Thermo Fisher Scientific. It is a device designed for specific laboratory tasks, but a detailed and unbiased description is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Cd45 j33, by Beckman Coulter (2 mentions)
Cd105 43a3, by BioLegend (2 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Rm4 5, by Thermo Fisher Scientific (1 mentions)
Ra3 6b2, by Thermo Fisher Scientific (1 mentions)
Mel 14, by BioLegend (1 mentions)
α cd8 5h10, by Thermo Fisher Scientific (1 mentions)
Uc10 4b9, by Thermo Fisher Scientific (1 mentions)
3 protocols using af6 120
1
Comprehensive Phenotyping of Mouse Splenocytes
2
Isolation and Characterization of Luci-eASCs
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Luci-eASCs were defined according to the criteria of the International Society for Cellular Therapy (43 (link)). Luci-eASCs were characterized by their immunophenotype; positive for CD73 (AD2), CD90 (5E10, both from Becton Dickinson), and CD105 (43A3, from Biolegend) and negative for CD14 (RM052, from Immunotech), CD34 (8G12, from Becton Dickinson), CD45 (J33, from Beckman Coulter), and HLA-DR (AF6-120.1, from eBiosciences).
3
Luci-eASCs Characterization Criteria
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Luci-eASCs were defined according to the criteria of the International Society for Cellular Therapy [36 (link)]. Luci-eASCs were stained using antibodies for CD73 (AD2), CD90 (5E10, both from Becton Dickinson), CD105 (43A3, Biolegend, San Diego, CA, USA), CD14 (RM052, Immunotech, Monrovia, CA, USA), CD34 (8G12, Becton Dickinson, Franklin Lakes, NJ, USA), CD45 (J33, Beckman Coulter, Brea, CA, USA) and HLA-DR (AF6-120.1, eBiosciences, Waltham, MA, USA) [18 (link)].
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