Rag2 il2rg

Mice used in this study were housed and bred at Memorial Sloan Kettering Cancer Center (MSKCC) under specific pathogen-free conditions. Experiments were conducted using age- and gender-matched mice (typically 6–10 wk old) in accordance with approved institutional protocols from the Institutional Animal Care and Use Committee at MSKCC. The strains used in this study, all on the C57BL/6 background, are listed below: C57BL/6 (CD45.2), B6.SJL (CD45.1), Adrb2^fl/fl (Hinoi et al., 2008 (link)), Klra8^−/− (Ly49H-deficient; Fodil-Cornu et al., 2008 (link)), Ncr1^gfp (Gazit et al., 2006 (link)), Nkp46iCre (referred to as NKp46^Cre; Narni-Mancinelli et al., 2011 (link)), Vav1-iCre (referred to as Vav1^cre; The Jackson Laboratory), Adrb2^−/− (Chruscinski et al., 1999 (link)), Rag2^−/−Il2rg^−/− (Taconic), Rosa26^tdTom (The Jackson Laboratory), Stat1^−/− (Meraz et al., 1996 (link)), and Stat4^−/− (Kaplan et al., 1996 (link)). B6 CD45.1 × CD45.2, Klra8^−/− CD45.1 × CD45.2, NKp46^Cre× Rosa26^tdTom, and NKp46^Cre× Adrb2^fl/fl mice were generated at MSKCC. Adrb2^fl/fl mice were generously provided by Dr. Daniel Mucida (The Rockefeller University, New York, NY) with the permission of Dr. Gerard Karsenty (Columbia University, New York, NY). Adoptive transfer studies and the generation of mixed bone marrow chimeric mice were performed as previously described (Beaulieu et al., 2014 (link); Sun et al., 2009 (link)).

Wild-type (WT) C57BL/6 (Jackson Laboratory), Casp1^−/− (Rauch et al., 2017 (link)), Nlrc4^−/−Asc^−/− (Aachoui et al., 2015 (link)), Casp^−/− (Kayagaki et al., 2011 (link)), Casp1^−/−Casp11^{129mut/129mut} referred to as Casp1^−/−Casp11^−/− (Kuida et al., 1995 (link)), Elane^−/− (Jackson # 006112), Mpo^−/− (Jackson Laboratory # 004265), Ncf1^mt/mt referred to as Ncf1^−/− (Jackson # 004742), Prf1^−/− (Jackson # 002407), Rag1^−/− (Jackson # 002216), Rag2^−/−Il2rg^−/− (Taconic # 4111), Gsdmd^−/− (Rauch et al., 2017 (link)), Ifng^−/− (Jackson # 002287), Casp11^fl/fl (Cheng et al., 2017 (link)), Mrp8-cre (Jackson # 021614), and LysM-cre (Jackson # 004781) mice were used in this study. All mice were 6-12 weeks old, male or female, and housed under specific pathogen free condition-free facilities. All protocols were approved by the Institutional Animal Care and Use Committee at the University of Arkansas for medical Sciences at Little Rock, or Institutional Animal Care and Use Committee at the University of North Carolina at Chapel Hill.

All mice used in this study were bred and maintained at Memorial Sloan Kettering Cancer Center (MSKCC) in accordance with all guidelines of the Institutional Animal Care and Use Committee. This study used the following mouse strains, all on the C57BL/6 genetic background: C57BL/6 (CD45.2; The Jackson Laboratory), B6.SJL (CD45.1; Taconic), Ifnar1^−/− (Müller et al., 1994 (link)), Stat1^−/− (Meraz et al., 1996 (link)), Klra8^−/− (Ly49H deficient; Fodil-Cornu et al., 2008 (link)), Nkp46^iCre (referred to as NKp46^Cre; Narni-Mancinelli et al., 2011 (link)), B2m^−/− (Taconic), Rag2^−/−Il2rg^−/− (Taconic), Ncr1^gfp/gfp (Gazit et al., 2006 (link)), Prf1^−/− (The Jackson Laboratory), and R26^DTA (The Jackson Laboratory). NKp46^Crex R26^DTA mice were generated at MSKCC. Adoptive transfer studies and the generation of mixed bone marrow chimeric mice were performed as previously described (Sun et al., 2009 (link)). Bone marrow chimeric mice were infected by i.p. injections of 7.5 × 10³ PFU of salivary gland–derived Smith strain MCMV. Mice used in adoptive transfer studies were infected with 7.5 × 10² PFU of MCMV. Newborn Ly49H-deficient mice were infected with 2 × 10³ PFU of MCMV. LCMV infection was performed as described previously (Sun et al., 2004 (link)). In vivo blockade of NKG2D signaling was accomplished by i.p. injection of anti-NKG2D (clone CX5; 200 µg/mouse) on day 0 of LCMV infection.