Laminb1 d9v6h

CH-223191 was purchased from Selleck. L-kynurenine and BAY11-7085 were purchased from MedChemExpress. Antibodies were used as following: AHR (D5S6H; Cell Signaling Technology), RelA (D14E12; Cell Signaling Technology), IκBα (L35A5; Cell Signaling Technology), phospho-IκBα (14D4; Cell Signaling Technology), α-Tubulin (DM1A; Cell Signaling Technology), LaminB1 (D9V6H; Cell Signaling Technology), HTLV-1 Tax (1A3; Abcam), HTLV-1 gp46 (67/5.5.13.1; Abcam), HTLV-1 p24 (46/3.24.4; Abcam), HTLV-1 p19 (TP-7; Abcam), β-Actin (AF0003; Beyotime Biotechnology), GAPDH (AF0006; Beyotime Biotechnology).

Equal amounts (20 μg or 40 μg) of the cell extracts were separated on a 10% or 12% dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel. The blotting membranes were probed using an antiserum of ARv7 and ARFL (MA5-13426, Invitrogen, Carlsbad, CA, USA), MALT1 (EP603Y, Abcam, Cambridge, MA, USA), NF-κB p50 (06-886, Merck Millipore, Burlington, MA, USA), NF-κB p65 (06-418, Merck Millipore, Burlington, MA, USA), Lamin B1 (D9V6H, Cell signaling Technology Inc., Danvers, MA, USA), IκB-α (#9242, Cell signaling Technology Inc.), p-IκB-α (#2859, Cell signaling Technology Inc.), GAPDH (6C5, Santa Cruz Biotechnology, Dallas, TX, USA), NDRG1 (42-6200, Thermo Fisher Scientific Inc., Vilnius, Lithuania), PSA (A0562, Dako Denmark A/S, Glostrup, Denmark), or β-actin (T0022, Affinity bioscience, Cincinnati, OH, USA). The band intensities in the blot membrane were detected by the Western Lightning^TM Plus Chemiluminescence detection system (PerkinElmer Inc., Waltham, MD, USA), recorded by the LuminoGraph II chemiluminescent imaging system (Atto Corporation, Tokyo, Japan), and analyzed by the Image J software (version 1.52a).

Antibodies for p53 (10442-1-AP) were purchased from Proteintech (Chicago, IL, USA). ESD (sc-134333), goat anti-mouse horseradish peroxidase (HRP)-conjugated IgG (GP016129) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). JAB1 (ab124720) was purchased from Abcam. GAPDH (G8795) and β-actin (ACTB, A1978) were purchased from Sigma-Aldrich (Burlington, MA, USA). Lamin A/C (4C11) and Lamin B1 (D9V6H) were from Cell Signaling Technology (Danvers, MA, USA). Propidium iodide solution (PI, P4864) was from Sigma-Aldrich (Burlington, MA, USA). Recombinant RNase A (B600476-0200) was purchased from Sangon Biotech (Shanghai, China).
The small-molecule chemical FPD5 was provided by Professor Baoxiang Zhao (Shandong University, Jinan). The synthetic protocols have been published.