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Anti rock

Manufactured by Cell Signaling Technology
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Anti-ROCK is a laboratory product that targets ROCK (Rho-associated coiled-coil containing protein kinase) proteins. ROCK proteins are essential regulators of the cytoskeleton and cell motility. Anti-ROCK can be used to study the role of ROCK in various cellular processes.

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Lab products found in correlation

Anti rhoa, by Cell Signaling Technology (2 mentions) Anti rhoc, by Cell Signaling Technology (2 mentions) Anti α tubulin antibody, by Cell Signaling Technology (1 mentions) Simvastatin sim, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Oleic acid, by Merck Group (1 mentions) Creatinine, by Nanjing Jiancheng (1 mentions) Cell counting kit 8 cck 8, by Nanjing Jiancheng (1 mentions) Diagnostic assay kit, by Nanjing Jiancheng (1 mentions) Anti p yap, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Anti tlr4, by Santa Cruz Biotechnology (1 mentions) Anti pi3k, by Cell Signaling Technology (1 mentions) Anti p pi3k, by Cell Signaling Technology (1 mentions) Anti nf κb, by Cell Signaling Technology (1 mentions) Anti yap, by Cell Signaling Technology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti p nf κb, by Cell Signaling Technology (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions)

4 protocols using anti rock

1

Endophytic Aspergillus terreus Derived Anti-RhoA Compounds

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FC (≥ 99.5%) was collected from endophytic Aspergillus terreus (strain No. FC118) via the root of Rhizophora stylosa (Rhizophoraceae). The anti-RhoA, anti-prenyl, anti-ROCK, anti-sterol response element binding protein-1c (Srebp-1c), anti-α-tubulin antibodies, and secondary antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Oleic acid, palmitic acid, and simvastatin (Sim) was purchased from Sigma Aldrich Company (St. Louis, MO, USA). Total cholesterol (TC), Triglyceride (TG), low density lipoprotein-cholesterol (LDL-c), high density lipoprotein-cholesterol (HDL-c), free fatty acid (FFA), total bilirubin (T-bili), alanine transaminase (ALT), aspartate transaminase (AST), creatinine, creatine kinase, and Cell Counting Kit-8 (CCK-8) were measured by diagnostic assay kits from Nanjing Jiancheng Company (NJ, China).
Yu W., Gao Y., Zhao Z., Long X., Yi Y, & Ai S. (2023). Fumigaclavine C ameliorates liver steatosis by attenuating hepatic de novo lipogenesis via modulation of the RhoA/ROCK signaling pathway. BMC Complementary Medicine and Therapies, 23, 288.
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2

Investigating NF-κB Signaling Pathway

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PB2 (purity > 90%), DEX (used as a positive control), and LPS derived from bacteria (serotype: 0111:B4, L5293) were purchased from Sigma (St. Louis, MO, USA). Anti-NF-κB, anti-p-NF-κB, anti-Akt, anti-p-Akt, anti-PI3K, anti-p-PI3K, anti-YAP, anti-p-YAP, anti-ROCK, anti-Rhoc, and anti-β-actin antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-TLR4 and anti-p-LATS1/2 (phospho-Ser909/872) antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA) and MyBioSource (San Diego, CA, USA), respectively.
Kim G.O., Park D.H, & Bae J.S. (2023). Procyanidin B2 Attenuates Sepsis-Induced Acute Lung Injury via Regulating Hippo/Rho/PI3K/NF-κB Signaling Pathway. International Journal of Molecular Sciences, 24(9), 7930.
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3

Molecular Regulation of Cellular Dynamics

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Chemicals were obtained from the following sources: bovine serum albumin (BSA), progesterone, TRITC-labeled phalloidin, and calcium ionophore A23187 were purchased from Sigma (St. Louis, MO); membrane-permeable Exoenzyme C3 Transferase (C4) was obtained from Cytoskeleton (Denver, CO); Y-27632 was acquired from Cayman Chemicals (Ann Arbor, MI); InSolution RAC1 inhibitor (CAS 1177865-17-6) and H89 from Calbiochem; IPA-3, dbcAMP and IBMX from Sigma and BMS-3 from SynKinase; Anti-phosphotyrosine (pY) monoclonal antibody (clone 4G10) was obtained from Upstate Biotechnology (Lake Placid, NY); anti-RHOA, anti-RHOB, anti-RHOC, anti-ROCK, anti-PAK1, anti-LIMK1, anti- LIMK2, anti-Cofilin, anti-phospho-LIMK1/2 (pLIMK1/2) and anti-phospho- Cofilin (pCofilin) antibodies were purchased from Cell Signaling (Danvers, MA); Anti-β-Tubulin monoclonal antibody was obtained from Sigma. Anti-RAC1 and anti-ACTIN antibodies and propidium iodide were purchased from Santa Cruz (Santa Cruz, CA); Horseradish peroxidase-conjugated anti-mouse and anti-rabbit IgG were purchased from Vector and Cell Signaling respectively.
Romarowski A., Battistone M.A., La Spina F.A., Puga Molina L.D., Luque G.M., Vitale A.M., Cuasnicu P.S., Visconti P.E., Krapf D, & Buffone M.G. (2015). PKA-dependent phosphorylation of LIMK1 and Cofilin is essential for mouse sperm acrosomal exocytosis. Developmental biology, 405(2), 237-249.
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4

Inhibition of Fibrosis Pathways

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HE, PAS and Masson's trichrome staining kits were purchased from Nanjing Jiancheng Biology Engineering Institute (Nanjing, Jiangsu, China). Dulbecco's modified Eagle's medium (DMEM), foetal bovine serum (FBS), penicillin and streptomycin were purchased from GIBCO BRL. Thrombin and dexamethasone powder were from Sigma‐Aldrich Co. Dabigatran was obtained from Boehringer Ingelheim Pharma. Inhibitors used were PAR1 antagonist SCH79797 (Tocris Bioscience), RhoA inhibitor Y‐16, ROCK inhibitor Y‐27632, YAP inhibitor verteporfin (MedChemExpress) and the actin polymerization inhibitor Cytochalasin D (Abcam). The antibodies used were anti‐collagen I, anti‐CTGF, anti‐β‐actin, anti‐GAPDH (Biosynthesis Biotechnology); anti‐RhoA, anti‐ROCK, anti‐phosphorylated MLC2, anti‐MLC2, anti‐phosphorylated SMAD2, anti‐SMAD2, anti‐phosphorylated YAP, anti‐α‐SMA, anti‐cyclin D1 (Cell Signaling Technology) and anti‐YAP (Santa Cruz Biotechnology).
Deng Z., Xie H., Cheng W., Zhang M., Liu J., Huo Y., Liao Y, & Cheng Y. (2020). Dabigatran ameliorates airway smooth muscle remodeling in asthma by modulating Yes‐associated protein. Journal of Cellular and Molecular Medicine, 24(14), 8179-8193.
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