Rabbit polyclonal anti tyrosine hydroxylase antibody

Apocynin, bovine serum albumin, N-3-(aminomethyl)benzylacetamide ·2HCl (1400W), Maneb (Manganese ethylene-1,2-bisdithiocarbamate), DAB, NADPH, paraquat ( 1,1-dimethyl-4,4-bipyridinium), paraformaldehyde, Triton X-100 , and 5,10,15,20,-tetrakis(4-sulfonatophenyl) porphyrinato iron (III) chloride (FeTPPS) were from Sigma (St. Louis, MO). 5, 5-dimethyl-1-pyrroline N-oxide (DMPO) was obtained from Dojindo Laboratories (Rockville, MD) and used without further purification. Chicken and rabbit polyclonal anti-DMPO antibodies were developed in our laboratory and used in the immuno-spin trapping studies. Mouse monoclonal anti-integrin □M (ox-42), rabbit polyclonal anti-iNOS, and P67 phox antibodies were from Santa Cruz Biotechnology (Dallas, TX). Mouse monoclonal anti-alpha synuclein, and anti-β-actin antibodies were from Abcam (Cambridge, MA). Rabbit polyclonal anti-tyrosine hydroxylase antibody was from Millipore (Billerica, MA). Micro BCA Protein Assay Kit, Permount, RIPA buffer, Surfact-Amps X-100, and Pierce Classic Immunoprecipitation Kits were from Thermo Scientific (Rockford, IL). Nitrocellulose membranes, Prolong Gold anti-fade reagent with DAPI and AlexaFluor secondary antibodies were from Invitrogen (Grand Island, NY). The Vectastain Elite abc kit was from Vector laboratories (Peterborough, UK).