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Hrp labeled anti igg1 antibodies

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HRP-labeled anti-IgG1 antibodies are a type of laboratory reagent used in various immunoassay techniques. They are designed to detect and bind to the IgG1 subclass of immunoglobulins, with the horseradish peroxidase (HRP) enzyme attached to facilitate signal detection.

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Lab products found in correlation

Hel antigen, by Merck Group (2 mentions) Ovalbumin (ova), by Merck Group (2 mentions) Spectramax m5, by Molecular Devices (2 mentions) Bovine serum albumin (bsa), by Research Products International (2 mentions) Np1 rsa, by LGC (2 mentions) Np25 bsa, by LGC (2 mentions) Np10 bsa, by LGC (2 mentions)

2 protocols using hrp labeled anti igg1 antibodies

1

Evaluation of Antigen-Specific IgG1 Antibodies

Cited 1 time
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Serum was harvested from blood collected by lateral tail vein sampling or cardiac puncture postmortem. OVA-specific and NP-specific IgG1 ELISA was performed as described (Brooks et al., 2020 (link); Tan et al., 2020 (link)). Briefly, ELISA plates were coated with NP conjugates (NP1-RSA, NP25-BSA, NP10-BSA all from LGC Biosearch; NP3-OVA, NP7-OVA, NP9-OVA and NP19-OVA were conjugated in-house as described above), OVA (Sigma Aldrich), BSA (Research Products International) or HEL antigen (10μg/mL; Sigma Aldrich), samples were added, and HRP-labeled anti-IgG1 antibodies (Southern Biotech) were used to detect plate-bound IgG1. Plates were developed with slow kinetic form TMB (Sigma Aldrich) and stopped with 1N sulfuric acid. Absorbance was measured at 450nm using spectrophotometer (SpectraMax M5, Molecular Devices). Relative titers were interpolated from standard curves generated using samples with known high titers of antigen-specific antibody.
Brooks J.F., Tan C., Mueller J.L., Hibiya K., Hiwa R., Vykunta V, & Zikherman J. (2021). Negative feedback by NUR77/Nr4a1 restrains B cell clonal dominance during early T-dependent immune responses. Cell reports, 36(9), 109645.
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2

Evaluation of Antigen-Specific IgG1 Antibodies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum was harvested from blood collected by lateral tail vein sampling or cardiac puncture postmortem. OVA-specific and NP-specific IgG1 ELISA was performed as described (Brooks et al., 2020 (link); Tan et al., 2020 (link)). Briefly, ELISA plates were coated with NP conjugates (NP1-RSA, NP25-BSA, NP10-BSA all from LGC Biosearch; NP3-OVA, NP7-OVA, NP9-OVA and NP19-OVA were conjugated in-house as described above), OVA (Sigma Aldrich), BSA (Research Products International) or HEL antigen (10μg/mL; Sigma Aldrich), samples were added, and HRP-labeled anti-IgG1 antibodies (Southern Biotech) were used to detect plate-bound IgG1. Plates were developed with slow kinetic form TMB (Sigma Aldrich) and stopped with 1N sulfuric acid. Absorbance was measured at 450nm using spectrophotometer (SpectraMax M5, Molecular Devices). Relative titers were interpolated from standard curves generated using samples with known high titers of antigen-specific antibody.
Brooks J.F., Tan C., Mueller J.L., Hibiya K., Hiwa R., Vykunta V, & Zikherman J. (2021). Negative feedback by NUR77/Nr4a1 restrains B cell clonal dominance during early T-dependent immune responses. Cell reports, 36(9), 109645.
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